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Characterization of the changes in eicosanoid profiles of activated macrophages treated with 20(S)-ginsenoside Rg3.

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Title: Characterization of the changes in eicosanoid profiles of activated macrophages treated with 20(S)-ginsenoside Rg3.
Authors: Lee, Jae Won1, Choi, Yu Ri2, Mok, Hyuck Jun2, Seong, Hyun-A3, Lee, Dae Young1, Kim, Geum-Soog1, Yoon, Ji Hye1, Kim, Kwang Pyo2 kimkp@khu.ac.kr, Kim, Hyung Don1,3 khd0303@rda.go.kr
Source: Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences. Oct2017, Vol. 1065/1066, p14-19. 6p.
Subjects: Eicosanoids, Ginsenosides, Macrophages, Liquid chromatography-mass spectrometry, Lipopolysaccharides, Tumor necrosis factors
Abstract: In this study, we used ultra-performance liquid chromatography coupled with tandem mass spectrometry to assess the levels of eicosanoids from RAW264.7 macrophages treated with lipopolysaccharides (LPS) and 20(S)-ginsenoside Rg3 (Rg3). The production of nitric oxide (NO) and the secretion of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were increased in inflammatory macrophages treated with LPS. Rg3 treatment, however, decreased the levels of NO, TNF-α, and IL-6 in activated macrophages. Eicosanoids, known as major metabolites correlated with inflammation, have pro- or anti-inflammatory activities. For a detailed characterization of the eicosanoids altered by treatment with LPS and Rg3, the eicosanoids were profiled by multiple reaction monitoring. A total of 69 macrophage eicosanoids were analyzed and the profiling dataset was statistically analyzed. Principal component and hierarchical cluster analyses differentiated control cells from cells treated with LPS, Rg3, or LPS + Rg3 for 12 or 24 h. Furthermore, 18 differentially regulated eicosanoids were found between macrophages treated with LPS for 24 h and those treated with LPS + Rg3 for 24 h (fold change > 2, p value < 0.05). These results indicate that Rg3 alters eicosanoid metabolism in activated macrophages treated with LPS. Furthermore, we also identified several eicosanoids correlated with the anti-inflammatory activity of Rg3. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 125787872
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
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  Data: Characterization of the changes in eicosanoid profiles of activated macrophages treated with 20(S)-ginsenoside Rg3.
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  Data: &lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Lee%2C+Jae+Won%22&quot;&gt;Lee, Jae Won&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Choi%2C+Yu+Ri%22&quot;&gt;Choi, Yu Ri&lt;/searchLink&gt;&lt;relatesTo&gt;2&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Mok%2C+Hyuck+Jun%22&quot;&gt;Mok, Hyuck Jun&lt;/searchLink&gt;&lt;relatesTo&gt;2&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Seong%2C+Hyun-A%22&quot;&gt;Seong, Hyun-A&lt;/searchLink&gt;&lt;relatesTo&gt;3&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Lee%2C+Dae+Young%22&quot;&gt;Lee, Dae Young&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Kim%2C+Geum-Soog%22&quot;&gt;Kim, Geum-Soog&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Yoon%2C+Ji+Hye%22&quot;&gt;Yoon, Ji Hye&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Kim%2C+Kwang+Pyo%22&quot;&gt;Kim, Kwang Pyo&lt;/searchLink&gt;&lt;relatesTo&gt;2&lt;/relatesTo&gt;&lt;i&gt; kimkp@khu.ac.kr&lt;/i&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Kim%2C+Hyung+Don%22&quot;&gt;Kim, Hyung Don&lt;/searchLink&gt;&lt;relatesTo&gt;1,3&lt;/relatesTo&gt;&lt;i&gt; khd0303@rda.go.kr&lt;/i&gt;
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  Data: &lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Eicosanoids%22&quot;&gt;Eicosanoids&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Ginsenosides%22&quot;&gt;Ginsenosides&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Macrophages%22&quot;&gt;Macrophages&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Liquid+chromatography-mass+spectrometry%22&quot;&gt;Liquid chromatography-mass spectrometry&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Lipopolysaccharides%22&quot;&gt;Lipopolysaccharides&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Tumor+necrosis+factors%22&quot;&gt;Tumor necrosis factors&lt;/searchLink&gt;
– Name: Abstract
  Label: Abstract
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  Data: In this study, we used ultra-performance liquid chromatography coupled with tandem mass spectrometry to assess the levels of eicosanoids from RAW264.7 macrophages treated with lipopolysaccharides (LPS) and 20(S)-ginsenoside Rg3 (Rg3). The production of nitric oxide (NO) and the secretion of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were increased in inflammatory macrophages treated with LPS. Rg3 treatment, however, decreased the levels of NO, TNF-α, and IL-6 in activated macrophages. Eicosanoids, known as major metabolites correlated with inflammation, have pro- or anti-inflammatory activities. For a detailed characterization of the eicosanoids altered by treatment with LPS and Rg3, the eicosanoids were profiled by multiple reaction monitoring. A total of 69 macrophage eicosanoids were analyzed and the profiling dataset was statistically analyzed. Principal component and hierarchical cluster analyses differentiated control cells from cells treated with LPS, Rg3, or LPS + Rg3 for 12 or 24 h. Furthermore, 18 differentially regulated eicosanoids were found between macrophages treated with LPS for 24 h and those treated with LPS + Rg3 for 24 h (fold change &gt; 2, p value &lt; 0.05). These results indicate that Rg3 alters eicosanoid metabolism in activated macrophages treated with LPS. Furthermore, we also identified several eicosanoids correlated with the anti-inflammatory activity of Rg3. [ABSTRACT FROM AUTHOR]
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  Data: &lt;i&gt;Copyright of Journal of Chromatography B: Analytical Technologies in the Biomedical &amp; Life Sciences is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder&#39;s express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.&lt;/i&gt; (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1016/j.jchromb.2017.09.002
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      – Code: eng
        Text: English
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      Pagination:
        PageCount: 6
        StartPage: 14
    Subjects:
      – SubjectFull: Eicosanoids
        Type: general
      – SubjectFull: Ginsenosides
        Type: general
      – SubjectFull: Macrophages
        Type: general
      – SubjectFull: Liquid chromatography-mass spectrometry
        Type: general
      – SubjectFull: Lipopolysaccharides
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      – SubjectFull: Tumor necrosis factors
        Type: general
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      – TitleFull: Characterization of the changes in eicosanoid profiles of activated macrophages treated with 20(S)-ginsenoside Rg3.
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            NameFull: Lee, Jae Won
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            NameFull: Choi, Yu Ri
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              Text: Oct2017
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              Y: 2017
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              Value: 1065/1066
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