Attack-Phase Bdellovibrio bacteriovorus Responses to Extracellular Nutrients Are Analogous to Those Seen During Late Intraperiplasmic Growth.
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| Title: | Attack-Phase Bdellovibrio bacteriovorus Responses to Extracellular Nutrients Are Analogous to Those Seen During Late Intraperiplasmic Growth. |
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| Authors: | Dwidar, Mohammed, Im, Hansol1, Mitchell, Robert1, Seo, Jeong2 |
| Source: | Microbial Ecology. Nov2017, Vol. 74 Issue 4, p937-946. 10p. |
| Subjects: | Bdellovibrio bacteriovorus, Nutrient interactions, RNA sequencing, Biosynthesis, Biodegradation, Predation |
| Abstract: | Bdellovibrio bacteriovorus is a predatory bacterium which lives by invading the periplasm of gram-negative bacteria and consuming them from within. This predator was thought to be dependent upon prey for nutrients since it lacks genes encoding for critical enzymes involved in amino acid biosynthesis. This study, however, found that planktonic attack-phase predators are not just dependent upon prey for nutrients, but rather, they respond to nutrients in the surrounding medium and, subsequently, synthesize and secrete proteases in a nutrient-dependent manner. The major secreted proteases were identified through mass spectrometry analyses. Subsequent RT-qPCR analyses found that the nutrient-induced proteases are similar to those expressed within the prey periplasm during the late intraperiplasmic growth phase. Furthermore, RNA sequencing found that incubating the planktonic attack-phase cells in a nutritious environment for a short period of time (4 h) changes its gene expression pattern to a status that is akin to the late intraperiplasmic phase, with more than 94% of the genes previously identified as being late intraperiplasmic-specific also being induced by nutrient broth in this study. This strong correlation between the gene expression patterns hints that the availability of hydrolyzed prey cell components to the predator is likely the stimulus controlling the expression of late intraperiplasmic B. bacteriovorus genes during predation. [ABSTRACT FROM AUTHOR] |
| Copyright of Microbial Ecology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Items | – Name: Title Label: Title Group: Ti Data: Attack-Phase Bdellovibrio bacteriovorus Responses to Extracellular Nutrients Are Analogous to Those Seen During Late Intraperiplasmic Growth. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Dwidar%2C+Mohammed%22">Dwidar, Mohammed</searchLink><br /><searchLink fieldCode="AR" term="%22Im%2C+Hansol%22">Im, Hansol</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Mitchell%2C+Robert%22">Mitchell, Robert</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Seo%2C+Jeong%22">Seo, Jeong</searchLink><relatesTo>2</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Microbial+Ecology%22">Microbial Ecology</searchLink>. Nov2017, Vol. 74 Issue 4, p937-946. 10p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Bdellovibrio+bacteriovorus%22">Bdellovibrio bacteriovorus</searchLink><br /><searchLink fieldCode="DE" term="%22Nutrient+interactions%22">Nutrient interactions</searchLink><br /><searchLink fieldCode="DE" term="%22RNA+sequencing%22">RNA sequencing</searchLink><br /><searchLink fieldCode="DE" term="%22Biosynthesis%22">Biosynthesis</searchLink><br /><searchLink fieldCode="DE" term="%22Biodegradation%22">Biodegradation</searchLink><br /><searchLink fieldCode="DE" term="%22Predation%22">Predation</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Bdellovibrio bacteriovorus is a predatory bacterium which lives by invading the periplasm of gram-negative bacteria and consuming them from within. This predator was thought to be dependent upon prey for nutrients since it lacks genes encoding for critical enzymes involved in amino acid biosynthesis. This study, however, found that planktonic attack-phase predators are not just dependent upon prey for nutrients, but rather, they respond to nutrients in the surrounding medium and, subsequently, synthesize and secrete proteases in a nutrient-dependent manner. The major secreted proteases were identified through mass spectrometry analyses. Subsequent RT-qPCR analyses found that the nutrient-induced proteases are similar to those expressed within the prey periplasm during the late intraperiplasmic growth phase. Furthermore, RNA sequencing found that incubating the planktonic attack-phase cells in a nutritious environment for a short period of time (4 h) changes its gene expression pattern to a status that is akin to the late intraperiplasmic phase, with more than 94% of the genes previously identified as being late intraperiplasmic-specific also being induced by nutrient broth in this study. This strong correlation between the gene expression patterns hints that the availability of hydrolyzed prey cell components to the predator is likely the stimulus controlling the expression of late intraperiplasmic B. bacteriovorus genes during predation. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Microbial Ecology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00248-017-1003-1 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 10 StartPage: 937 Subjects: – SubjectFull: Bdellovibrio bacteriovorus Type: general – SubjectFull: Nutrient interactions Type: general – SubjectFull: RNA sequencing Type: general – SubjectFull: Biosynthesis Type: general – SubjectFull: Biodegradation Type: general – SubjectFull: Predation Type: general Titles: – TitleFull: Attack-Phase Bdellovibrio bacteriovorus Responses to Extracellular Nutrients Are Analogous to Those Seen During Late Intraperiplasmic Growth. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Dwidar, Mohammed – PersonEntity: Name: NameFull: Im, Hansol – PersonEntity: Name: NameFull: Mitchell, Robert – PersonEntity: Name: NameFull: Seo, Jeong IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 11 Text: Nov2017 Type: published Y: 2017 Identifiers: – Type: issn-print Value: 00953628 Numbering: – Type: volume Value: 74 – Type: issue Value: 4 Titles: – TitleFull: Microbial Ecology Type: main |
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