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Carbohydrate oxidases in ericoid and ectomycorrhizal fungi: a possible source of Fenton radicals during the degradation of lignocellulose.

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Title: Carbohydrate oxidases in ericoid and ectomycorrhizal fungi: a possible source of Fenton radicals during the degradation of lignocellulose.
Authors: Burke, R. M.1, Cairney, J. W. G.2
Source: New Phytologist. Aug98, Vol. 139 Issue 4, p637-645. 9p.
Subjects: Mycorrhizal fungi, Fenton's reagent, Hydroxyl group, Lignocellulose, Organic compounds, Parasitic plants
Abstract: Isolates of the ericoid mycorrhizal fungus Hymenoscyphus ericae (Read) Korf et Kernan, and the ectomycorrhizal fungi Suillus variegatus (Swartz ex Fr.) and Pisolithus tinctorius (Pers.) Coker & Couch, along with a Cortinarius sp. and the white rot Phanerochaete chrysosporium Burdsall were examined for the ability to oxidize carbohydrates to their corresponding lactones and to excrete the H2O2 produced thereby. All except Phanerochaete chrysosporium were found to express cellobiose oxidase (cellobiose dehydrogenase, EC 1 . 1 . 19 . 88) and glucose oxidase (β-D-glucose : oxygen 1-oxidoreductase, EC 1 . 1 .3 . 4) when grown on cellobiose and glucose respectively. Production of extracellular H2O2 was visualized during growth on both substrates using ABTS as the chromogen. According to the Fenton reaction, H2O2 will react with hydrated or chelated Fe(II) in the environment to produce hydroxyl (Fenton) radicals, HO•. Mycelial extracts from each of the mycorrhizal fungi produced HO• in the presence of cellobiose and Fe(II), presumably mediated by H2O2 produced by cellobiose oxidase activity in the extracts. Conditions favourable to HO• production were shown to exist in Modified Melin-Norkrans medium, and the data discussed in relation to previously observed lignin degradation by mycorrhizal fungi. [ABSTRACT FROM AUTHOR]
Copyright of New Phytologist is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
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  Data: Carbohydrate oxidases in ericoid and ectomycorrhizal fungi: a possible source of Fenton radicals during the degradation of lignocellulose.
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  Data: <searchLink fieldCode="DE" term="%22Mycorrhizal+fungi%22">Mycorrhizal fungi</searchLink><br /><searchLink fieldCode="DE" term="%22Fenton's+reagent%22">Fenton's reagent</searchLink><br /><searchLink fieldCode="DE" term="%22Hydroxyl+group%22">Hydroxyl group</searchLink><br /><searchLink fieldCode="DE" term="%22Lignocellulose%22">Lignocellulose</searchLink><br /><searchLink fieldCode="DE" term="%22Organic+compounds%22">Organic compounds</searchLink><br /><searchLink fieldCode="DE" term="%22Parasitic+plants%22">Parasitic plants</searchLink>
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  Data: Isolates of the ericoid mycorrhizal fungus <em>Hymenoscyphus ericae</em> (Read) Korf et Kernan, and the ectomycorrhizal fungi <em>Suillus variegatus</em> (Swartz ex Fr.) and <em>Pisolithus tinctorius</em> (Pers.) Coker & Couch, along with a <em>Cortinarius</em> sp. and the white rot <em>Phanerochaete chrysosporium</em> Burdsall were examined for the ability to oxidize carbohydrates to their corresponding lactones and to excrete the H2O2 produced thereby. All except <em>Phanerochaete chrysosporium</em> were found to express cellobiose oxidase (cellobiose dehydrogenase, EC 1 . 1 . 19 . 88) and glucose oxidase (β-D-glucose : oxygen 1-oxidoreductase, EC 1 . 1 .3 . 4) when grown on cellobiose and glucose respectively. Production of extracellular H2O2 was visualized during growth on both substrates using ABTS as the chromogen. According to the Fenton reaction, H2O2 will react with hydrated or chelated Fe(II) in the environment to produce hydroxyl (Fenton) radicals, HO•. Mycelial extracts from each of the mycorrhizal fungi produced HO• in the presence of cellobiose and Fe(II), presumably mediated by H2O2 produced by cellobiose oxidase activity in the extracts. Conditions favourable to HO• production were shown to exist in Modified Melin-Norkrans medium, and the data discussed in relation to previously observed lignin degradation by mycorrhizal fungi. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of New Phytologist is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1046/j.1469-8137.1998.00235.x
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        Text: English
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        PageCount: 9
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      – SubjectFull: Mycorrhizal fungi
        Type: general
      – SubjectFull: Fenton's reagent
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      – SubjectFull: Hydroxyl group
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      – SubjectFull: Lignocellulose
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      – SubjectFull: Organic compounds
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      – SubjectFull: Parasitic plants
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              Text: Aug98
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