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Aqueous size-exclusion chromatography of polyelectrolytes on reversed-phase and hydrophilic interaction chromatography columns.

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Title: Aqueous size-exclusion chromatography of polyelectrolytes on reversed-phase and hydrophilic interaction chromatography columns.
Authors: Caltabiano, Anna M.1,2 caltanna@netscape.net, Foley, Joe P.2, Striegel, André M.3
Source: Journal of Chromatography A. Jan2018, Vol. 1532, p161-174. 14p.
Subjects: Polyelectrolytes, Aqueous solutions, Chromatographic analysis, Hydrophilic interactions, Separation (Technology), Temperature measurements
Abstract: The size-exclusion separation of a water-soluble polyelectrolyte polymer, sodium polystyrene sulfonate (NaPSS), was demonstrated on common reversed-phase (C 18, C 4 , phenyl, and cyano) and hydrophilic interaction chromatography (HILIC) columns. The effect of common solvents – acetonitrile (ACN), tetrahydrofuran (THF), and methanol (MeOH), used as mobile phase modifiers – on the elution of NaPSS and the effect of column temperature (within a relatively narrow range corresponding to typical chromatographic conditions, i.e. , 10 °C–60 °C) on the partition coefficient, K S E C , were also investigated. Non-size-exclusion chromatography (non-SEC) effects can be minimized by the addition of an electrolyte and an organic modifier to the mobile phase, and by increasing the column temperature ( e.g., to 50 °C or 60 °C). Strong solvents such as THF and ACN are more successful in the reduction of such effects than is the weaker solvent MeOH. The best performance is seen on medium polarity and polar stationary phases, such as cyanopropyl- and diol-modified silica (HILIC), where the elution of the NaPSS polyelectrolyte is by a near-ideal SEC mechanism. Hydrophobic stationary phases, such as C 18 , C 4 , and phenyl, require a higher concentration of a strong solvent modifier (THF) in the mobile phase to reduce non-SEC interactions of the solute with the stationary phase. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 126898971
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PubTypeId: academicJournal
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  Label: Title
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  Data: Aqueous size-exclusion chromatography of polyelectrolytes on reversed-phase and hydrophilic interaction chromatography columns.
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  Data: <searchLink fieldCode="AR" term="%22Caltabiano%2C+Anna+M%2E%22">Caltabiano, Anna M.</searchLink><relatesTo>1,2</relatesTo><i> caltanna@netscape.net</i><br /><searchLink fieldCode="AR" term="%22Foley%2C+Joe+P%2E%22">Foley, Joe P.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Striegel%2C+André+M%2E%22">Striegel, André M.</searchLink><relatesTo>3</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Chromatography+A%22">Journal of Chromatography A</searchLink>. Jan2018, Vol. 1532, p161-174. 14p.
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  Data: <searchLink fieldCode="DE" term="%22Polyelectrolytes%22">Polyelectrolytes</searchLink><br /><searchLink fieldCode="DE" term="%22Aqueous+solutions%22">Aqueous solutions</searchLink><br /><searchLink fieldCode="DE" term="%22Chromatographic+analysis%22">Chromatographic analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Hydrophilic+interactions%22">Hydrophilic interactions</searchLink><br /><searchLink fieldCode="DE" term="%22Separation+%28Technology%29%22">Separation (Technology)</searchLink><br /><searchLink fieldCode="DE" term="%22Temperature+measurements%22">Temperature measurements</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: The size-exclusion separation of a water-soluble polyelectrolyte polymer, sodium polystyrene sulfonate (NaPSS), was demonstrated on common reversed-phase (C 18, C 4 , phenyl, and cyano) and hydrophilic interaction chromatography (HILIC) columns. The effect of common solvents – acetonitrile (ACN), tetrahydrofuran (THF), and methanol (MeOH), used as mobile phase modifiers – on the elution of NaPSS and the effect of column temperature (within a relatively narrow range corresponding to typical chromatographic conditions, i.e. , 10 °C–60 °C) on the partition coefficient, K S E C , were also investigated. Non-size-exclusion chromatography (non-SEC) effects can be minimized by the addition of an electrolyte and an organic modifier to the mobile phase, and by increasing the column temperature ( e.g., to 50 °C or 60 °C). Strong solvents such as THF and ACN are more successful in the reduction of such effects than is the weaker solvent MeOH. The best performance is seen on medium polarity and polar stationary phases, such as cyanopropyl- and diol-modified silica (HILIC), where the elution of the NaPSS polyelectrolyte is by a near-ideal SEC mechanism. Hydrophobic stationary phases, such as C 18 , C 4 , and phenyl, require a higher concentration of a strong solvent modifier (THF) in the mobile phase to reduce non-SEC interactions of the solute with the stationary phase. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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        Value: 10.1016/j.chroma.2017.12.007
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      – Code: eng
        Text: English
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        PageCount: 14
        StartPage: 161
    Subjects:
      – SubjectFull: Polyelectrolytes
        Type: general
      – SubjectFull: Aqueous solutions
        Type: general
      – SubjectFull: Chromatographic analysis
        Type: general
      – SubjectFull: Hydrophilic interactions
        Type: general
      – SubjectFull: Separation (Technology)
        Type: general
      – SubjectFull: Temperature measurements
        Type: general
    Titles:
      – TitleFull: Aqueous size-exclusion chromatography of polyelectrolytes on reversed-phase and hydrophilic interaction chromatography columns.
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            NameFull: Caltabiano, Anna M.
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            NameFull: Foley, Joe P.
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            NameFull: Striegel, André M.
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            – D: 12
              M: 01
              Text: Jan2018
              Type: published
              Y: 2018
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