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Functional magnetic nanoparticles–assisted electrochemical biosensor for eosinophil cationic protein in cell culture.

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Title: Functional magnetic nanoparticles–assisted electrochemical biosensor for eosinophil cationic protein in cell culture.
Authors: Lee, Cheng-Yu1, Wu, Li-Ping1, Chou, Tzu-Ting1, Hsieh, You-Zung1 yzhsieh@mail.nctu.edu.tw
Source: Sensors & Actuators B: Chemical. Mar2018, Vol. 257, p672-677. 6p.
Subjects: Proteins, Cytological techniques, Lysozymes, Biosensors, Glycosaminoglycans
Abstract: A low-cost electrochemical biosensor, assisted by a new type of functional magnetic nanoparticles (NPs), has been developed to detect eosinophil cationic protein (ECP), a known biomarker of asthma, in cell culture. The heparin-modified magnetic NPs were mixed with a sample solution containing ECP. After ECP had been captured by the NPs, a magnetic field was applied behind a graphite-based screen-printed electrode. The functional magnetic NPs were attracted to the electrode, raising the ECP concentration near its surface. Because of the use of the functional magnetic NPs, the difference in the signal was amplified when applying larger sample volumes for detection, thereby enhancing the sensitivity of the biosensor. This approach provided a linear range for the analysis of the logarithm of the ECP concentration from 1 to 1000 nM, with a coefficient of determination 0.992; the limit of detection was 0.30 nM. The fabricated biosensor displayed good recovery in a cell culture medium incubated with the Beas–2 B cell line. The ability to detect the concentration of ECP in a cell culture at any time point should be useful for explaining contradictory findings regarding the relationship between the initial ECP concentration and the cell line. [ABSTRACT FROM AUTHOR]
Copyright of Sensors & Actuators B: Chemical is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Functional magnetic nanoparticles–assisted electrochemical biosensor for eosinophil cationic protein in cell culture.
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  Data: <searchLink fieldCode="AR" term="%22Lee%2C+Cheng-Yu%22">Lee, Cheng-Yu</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Wu%2C+Li-Ping%22">Wu, Li-Ping</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Chou%2C+Tzu-Ting%22">Chou, Tzu-Ting</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Hsieh%2C+You-Zung%22">Hsieh, You-Zung</searchLink><relatesTo>1</relatesTo><i> yzhsieh@mail.nctu.edu.tw</i>
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  Data: <searchLink fieldCode="JN" term="%22Sensors+%26+Actuators+B%3A+Chemical%22">Sensors & Actuators B: Chemical</searchLink>. Mar2018, Vol. 257, p672-677. 6p.
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  Data: <searchLink fieldCode="DE" term="%22Proteins%22">Proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Cytological+techniques%22">Cytological techniques</searchLink><br /><searchLink fieldCode="DE" term="%22Lysozymes%22">Lysozymes</searchLink><br /><searchLink fieldCode="DE" term="%22Biosensors%22">Biosensors</searchLink><br /><searchLink fieldCode="DE" term="%22Glycosaminoglycans%22">Glycosaminoglycans</searchLink>
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  Data: A low-cost electrochemical biosensor, assisted by a new type of functional magnetic nanoparticles (NPs), has been developed to detect eosinophil cationic protein (ECP), a known biomarker of asthma, in cell culture. The heparin-modified magnetic NPs were mixed with a sample solution containing ECP. After ECP had been captured by the NPs, a magnetic field was applied behind a graphite-based screen-printed electrode. The functional magnetic NPs were attracted to the electrode, raising the ECP concentration near its surface. Because of the use of the functional magnetic NPs, the difference in the signal was amplified when applying larger sample volumes for detection, thereby enhancing the sensitivity of the biosensor. This approach provided a linear range for the analysis of the logarithm of the ECP concentration from 1 to 1000 nM, with a coefficient of determination 0.992; the limit of detection was 0.30 nM. The fabricated biosensor displayed good recovery in a cell culture medium incubated with the Beas–2 B cell line. The ability to detect the concentration of ECP in a cell culture at any time point should be useful for explaining contradictory findings regarding the relationship between the initial ECP concentration and the cell line. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of Sensors & Actuators B: Chemical is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.snb.2017.11.033
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      – Code: eng
        Text: English
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        Type: general
      – SubjectFull: Cytological techniques
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      – SubjectFull: Lysozymes
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      – SubjectFull: Biosensors
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      – SubjectFull: Glycosaminoglycans
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      – TitleFull: Functional magnetic nanoparticles–assisted electrochemical biosensor for eosinophil cationic protein in cell culture.
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              M: 03
              Text: Mar2018
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