Utilization of dissolved inorganic carbon (DIC) and the response of the marine flagellate Isochrysis galbana to carbon or nitrogen stress.
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| Title: | Utilization of dissolved inorganic carbon (DIC) and the response of the marine flagellate Isochrysis galbana to carbon or nitrogen stress. |
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| Authors: | Clark, D. R.1 bbclark@swansea.ac.uk, Merrett, M. J.1, Flynn, K. J.1 |
| Source: | New Phytologist. Dec99, Vol. 144 Issue 3, p463-470. 8p. |
| Subjects: | Carbon, Nitrogen, Carbonic anhydrase, Nutritional assessment, Glutamine, Research |
| Abstract: | The growth of the marine flagellate Isochrysis galbana was followed in batch cultures at four concentrations of dissolved inorganic carbon (DIC), from C-and N-replete lag phase into C-and/or N-deplete stationary phase. Organic buffers were omitted from the growth medium, and culture pH was maintained at 8.30±0.05 by the addition of acid or alkali. The responses of the flagellate to N stress included an increase in the C:N ratio, and decreases in the ratios of glutamine (Gln): glutamate (Gln) and Chl a:C, and the cell Chl a quota. Conversely, the responses to C stress included a decrease in the C:N ratio, and increases in the ratios of Gln:Glu and Chl a:C, and the cell Chl a quota. The relationship between carbon-specific growth rate (C-μ), and the concentration of extracellular DIC, [DIC]ext, exhibited Michaelis-Menten type kinetics with a half saturation constant, KG(DIC) of 81μM. Comparative studies of the diatom Phaeodactylum tricornutum showed similar results, although the value of KG(DIC) was lower at 30 μM. [ABSTRACT FROM AUTHOR] |
| Copyright of New Phytologist is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 12714990 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Utilization of dissolved inorganic carbon (DIC) and the response of the marine flagellate <em>Isochrysis galbana</em> to carbon or nitrogen stress. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Clark%2C+D%2E+R%2E%22">Clark, D. R.</searchLink><relatesTo>1</relatesTo><i> bbclark@swansea.ac.uk</i><br /><searchLink fieldCode="AR" term="%22Merrett%2C+M%2E+J%2E%22">Merrett, M. J.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Flynn%2C+K%2E+J%2E%22">Flynn, K. J.</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22New+Phytologist%22">New Phytologist</searchLink>. Dec99, Vol. 144 Issue 3, p463-470. 8p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Carbon%22">Carbon</searchLink><br /><searchLink fieldCode="DE" term="%22Nitrogen%22">Nitrogen</searchLink><br /><searchLink fieldCode="DE" term="%22Carbonic+anhydrase%22">Carbonic anhydrase</searchLink><br /><searchLink fieldCode="DE" term="%22Nutritional+assessment%22">Nutritional assessment</searchLink><br /><searchLink fieldCode="DE" term="%22Glutamine%22">Glutamine</searchLink><br /><searchLink fieldCode="DE" term="%22Research%22">Research</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The growth of the marine flagellate <em>Isochrysis galbana</em> was followed in batch cultures at four concentrations of dissolved inorganic carbon (DIC), from C-and N-replete lag phase into C-and/or N-deplete stationary phase. Organic buffers were omitted from the growth medium, and culture pH was maintained at 8.30±0.05 by the addition of acid or alkali. The responses of the flagellate to N stress included an increase in the C:N ratio, and decreases in the ratios of glutamine (Gln): glutamate (Gln) and Chl <em>a</em>:C, and the cell Chl <em>a</em> quota. Conversely, the responses to C stress included a decrease in the C:N ratio, and increases in the ratios of Gln:Glu and Chl <em>a</em>:C, and the cell Chl <em>a</em> quota. The relationship between carbon-specific growth rate (C-μ), and the concentration of extracellular DIC, [DIC]ext, exhibited Michaelis-Menten type kinetics with a half saturation constant, KG(DIC) of 81μM. Comparative studies of the diatom <em>Phaeodactylum tricornutum</em> showed similar results, although the value of KG(DIC) was lower at 30 μM. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of New Phytologist is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1046/j.1469-8137.1999.00539.x Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 8 StartPage: 463 Subjects: – SubjectFull: Carbon Type: general – SubjectFull: Nitrogen Type: general – SubjectFull: Carbonic anhydrase Type: general – SubjectFull: Nutritional assessment Type: general – SubjectFull: Glutamine Type: general – SubjectFull: Research Type: general Titles: – TitleFull: Utilization of dissolved inorganic carbon (DIC) and the response of the marine flagellate <em>Isochrysis galbana</em> to carbon or nitrogen stress. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Clark, D. R. – PersonEntity: Name: NameFull: Merrett, M. J. – PersonEntity: Name: NameFull: Flynn, K. J. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 12 Text: Dec99 Type: published Y: 1999 Identifiers: – Type: issn-print Value: 0028646X Numbering: – Type: volume Value: 144 – Type: issue Value: 3 Titles: – TitleFull: New Phytologist Type: main |
| ResultId | 1 |
