Utilization of dissolved inorganic carbon (DIC) and the response of the marine flagellate Isochrysis galbana to carbon or nitrogen stress.
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| Title: | Utilization of dissolved inorganic carbon (DIC) and the response of the marine flagellate Isochrysis galbana to carbon or nitrogen stress. |
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| Authors: | Clark, D. R.1 bbclark@swansea.ac.uk, Merrett, M. J.1, Flynn, K. J.1 |
| Source: | New Phytologist. Dec99, Vol. 144 Issue 3, p463-470. 8p. |
| Subjects: | Carbon, Nitrogen, Carbonic anhydrase, Nutritional assessment, Glutamine, Research |
| Abstract: | The growth of the marine flagellate Isochrysis galbana was followed in batch cultures at four concentrations of dissolved inorganic carbon (DIC), from C-and N-replete lag phase into C-and/or N-deplete stationary phase. Organic buffers were omitted from the growth medium, and culture pH was maintained at 8.30±0.05 by the addition of acid or alkali. The responses of the flagellate to N stress included an increase in the C:N ratio, and decreases in the ratios of glutamine (Gln): glutamate (Gln) and Chl a:C, and the cell Chl a quota. Conversely, the responses to C stress included a decrease in the C:N ratio, and increases in the ratios of Gln:Glu and Chl a:C, and the cell Chl a quota. The relationship between carbon-specific growth rate (C-μ), and the concentration of extracellular DIC, [DIC]ext, exhibited Michaelis-Menten type kinetics with a half saturation constant, KG(DIC) of 81μM. Comparative studies of the diatom Phaeodactylum tricornutum showed similar results, although the value of KG(DIC) was lower at 30 μM. [ABSTRACT FROM AUTHOR] |
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| Database: | Engineering Source |
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