View in EDS

Activatable clinical fluorophore-quencher antibody pairs as dual molecular probes for the enhanced specificity of image-guided surgery.

Saved in:
Bibliographic Details
Title: Activatable clinical fluorophore-quencher antibody pairs as dual molecular probes for the enhanced specificity of image-guided surgery.
Authors: Obaid, Girgis1, Spring, Bryan Q.2, Bano, Shazia1, Hasan, Tayyaba1,3 thasan@mgh.harvard.edu
Source: Journal of Biomedical Optics. Dec2017, Vol. 22 Issue 12, p121607-1-121607-6. 6p.
Subjects: Immunoglobulins, Molecular probes, Medical imaging systems, Cetuximab, Antineoplastic agents
Abstract: The emergence of fluorescently labeled therapeutic antibodies has given rise to molecular probes for image-guided surgery. However, the extraneous interstitial presence of an unbound and nonspecifically accumulated probe gives rise to false-positive detection of tumor tissue and margins. Thus, the concept of tumor-cell activation of smart probes provides a potentially superior mechanism of delineating tumor margins as well as small tumor deposits. The combination of molecular targeting with intracellular activation circumvents the presence of extracellular, nonspecific signals of targeted probe accumulation. Here, we present a demonstration of the clinical antibodies cetuximab (cet, anti-EGFR mAb) and trastuzumab (trast, anti-HER-2 mAb) conjugated to Alexa Fluor molecules and IRDye QC-1 quencher optimized at the ratio of 1∶2∶6 to provide the greatest degree of proteolytic fluorescence activation, synonymous with intracellular lysosomal degradation. The cet-AF-Q-C1 conjugate (1∶2∶6) provides up to 9.8-fold proteolytic fluorescence activation. By preparing a spectrally distinct, irrelevant sham IgG-AF-QC-1 conjugate, a dual-activatable probe approach is shown to enhance the specificity of imaging within an orthotopic AsPC-1 pancreatic cancer xenograft model. The dual-activatable approach warrants expedited clinical translation to improve the specificity of image-guided surgery by spectrally decomposing specific from nonspecific probe accumulation, binding, and internalization. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Biomedical Optics is the property of SPIE - International Society of Optical Engineering and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 127324305
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Activatable clinical fluorophore-quencher antibody pairs as dual molecular probes for the enhanced specificity of image-guided surgery.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Obaid%2C+Girgis%22">Obaid, Girgis</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Spring%2C+Bryan+Q%2E%22">Spring, Bryan Q.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Bano%2C+Shazia%22">Bano, Shazia</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Hasan%2C+Tayyaba%22">Hasan, Tayyaba</searchLink><relatesTo>1,3</relatesTo><i> thasan@mgh.harvard.edu</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Journal+of+Biomedical+Optics%22">Journal of Biomedical Optics</searchLink>. Dec2017, Vol. 22 Issue 12, p121607-1-121607-6. 6p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Immunoglobulins%22">Immunoglobulins</searchLink><br /><searchLink fieldCode="DE" term="%22Molecular+probes%22">Molecular probes</searchLink><br /><searchLink fieldCode="DE" term="%22Medical+imaging+systems%22">Medical imaging systems</searchLink><br /><searchLink fieldCode="DE" term="%22Cetuximab%22">Cetuximab</searchLink><br /><searchLink fieldCode="DE" term="%22Antineoplastic+agents%22">Antineoplastic agents</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The emergence of fluorescently labeled therapeutic antibodies has given rise to molecular probes for image-guided surgery. However, the extraneous interstitial presence of an unbound and nonspecifically accumulated probe gives rise to false-positive detection of tumor tissue and margins. Thus, the concept of tumor-cell activation of smart probes provides a potentially superior mechanism of delineating tumor margins as well as small tumor deposits. The combination of molecular targeting with intracellular activation circumvents the presence of extracellular, nonspecific signals of targeted probe accumulation. Here, we present a demonstration of the clinical antibodies cetuximab (cet, anti-EGFR mAb) and trastuzumab (trast, anti-HER-2 mAb) conjugated to Alexa Fluor molecules and IRDye QC-1 quencher optimized at the ratio of 1∶2∶6 to provide the greatest degree of proteolytic fluorescence activation, synonymous with intracellular lysosomal degradation. The cet-AF-Q-C1 conjugate (1∶2∶6) provides up to 9.8-fold proteolytic fluorescence activation. By preparing a spectrally distinct, irrelevant sham IgG-AF-QC-1 conjugate, a dual-activatable probe approach is shown to enhance the specificity of imaging within an orthotopic AsPC-1 pancreatic cancer xenograft model. The dual-activatable approach warrants expedited clinical translation to improve the specificity of image-guided surgery by spectrally decomposing specific from nonspecific probe accumulation, binding, and internalization. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Biomedical Optics is the property of SPIE - International Society of Optical Engineering and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=127324305
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1117/1.JBO.22.12.121607
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 6
        StartPage: 121607-1
    Subjects:
      – SubjectFull: Immunoglobulins
        Type: general
      – SubjectFull: Molecular probes
        Type: general
      – SubjectFull: Medical imaging systems
        Type: general
      – SubjectFull: Cetuximab
        Type: general
      – SubjectFull: Antineoplastic agents
        Type: general
    Titles:
      – TitleFull: Activatable clinical fluorophore-quencher antibody pairs as dual molecular probes for the enhanced specificity of image-guided surgery.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Obaid, Girgis
      – PersonEntity:
          Name:
            NameFull: Spring, Bryan Q.
      – PersonEntity:
          Name:
            NameFull: Bano, Shazia
      – PersonEntity:
          Name:
            NameFull: Hasan, Tayyaba
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 12
              Text: Dec2017
              Type: published
              Y: 2017
          Identifiers:
            – Type: issn-print
              Value: 10833668
          Numbering:
            – Type: volume
              Value: 22
            – Type: issue
              Value: 12
          Titles:
            – TitleFull: Journal of Biomedical Optics
              Type: main
ResultId 1