The effect of membrane softeners on rigidity of lipid vesicle bilayers: Derivation from vesicle size changes.
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| Title: | The effect of membrane softeners on rigidity of lipid vesicle bilayers: Derivation from vesicle size changes. |
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| Authors: | Elsayed, Mustafa M.A.1,2 mustafa.elsayed@alexpharmres.com, Ibrahim, Marwa M.2, Cevc, Gregor3 |
| Source: | Chemistry & Physics of Lipids. Jan2018, Vol. 210, p98-108. 11p. |
| Subjects: | Spectrum analysis, Monomolecular films, Deoxycholic acid, Micelles synthesis, Polyethylene glycol |
| Abstract: | Deformability is not just a fundamentally interesting vesicle characteristic; it is also the key determinant of vesicle ability to cross the skin barrier; i.e. skin penetrability. Development of bilayer vesicles for drug and vaccine delivery across the skin should hence involve optimization of this property, which is controllable by the concentration of bilayer softeners in or near the vesicle bilayers. To this end, we propose a simple method for quantifying the effect of bilayer softeners on deformability of bilayer vesicles. The method derives the bending rigidity of vesicle bilayers from vesicle size dependence on softener concentration. To exemplify the method, we studied mixtures of soybean phosphatidylcholine with anionic sodium deoxycholate, non-ionic polyoxyethylene (20) sorbitan oleyl ester (polysorbate 80), or non-ionic polyoxyethylene (20) oleyl ether (C 18:1 EO 20 , Brij ® 98). With each of the tested bilayer softeners, the bending rigidity of the resulting mixed-amphipat vesicle bilayers decreased quasi-exponentially as the concentration of the bilayer softener increased, as one would expect on theoretical ground. The bilayer bending rigidity reached low values, near the thermal stability limit, i.e. k B T , before vesicle transformation into non-vesicular aggregates began. For a soybean phosphatidylcholine concentration of 5.0 mmol kg −1 , the bilayer bending rigidity reached 1.5 k B T at the total deoxycholate concentration of 4.1 mmol kg −1 and 3.4 k B T at the total polysorbate 80 concentration of 2.0 mmol kg −1 . In the case of C 18:1 EO 20 , the bilayer bending rigidity reached 1.5 k B T at the bilayer surface occupancy α = 0.1. The dependence of vesicle size on bilayer softener concentration thus reveals vesicle transformation into different aggregate structures (such as mixed micelles with poor skin penetrability) and practically valuable information on vesicle deformability. Our results compare favorably with results of literature measurements. We provide practical guidance on using the new analytical method to optimize deformable vesicle formulations. [ABSTRACT FROM AUTHOR] |
| Copyright of Chemistry & Physics of Lipids is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 128002238 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: The effect of membrane softeners on rigidity of lipid vesicle bilayers: Derivation from vesicle size changes. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Elsayed%2C+Mustafa+M%2EA%2E%22">Elsayed, Mustafa M.A.</searchLink><relatesTo>1,2</relatesTo><i> mustafa.elsayed@alexpharmres.com</i><br /><searchLink fieldCode="AR" term="%22Ibrahim%2C+Marwa+M%2E%22">Ibrahim, Marwa M.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Cevc%2C+Gregor%22">Cevc, Gregor</searchLink><relatesTo>3</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Chemistry+%26+Physics+of+Lipids%22">Chemistry & Physics of Lipids</searchLink>. Jan2018, Vol. 210, p98-108. 11p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Spectrum+analysis%22">Spectrum analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Monomolecular+films%22">Monomolecular films</searchLink><br /><searchLink fieldCode="DE" term="%22Deoxycholic+acid%22">Deoxycholic acid</searchLink><br /><searchLink fieldCode="DE" term="%22Micelles+synthesis%22">Micelles synthesis</searchLink><br /><searchLink fieldCode="DE" term="%22Polyethylene+glycol%22">Polyethylene glycol</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Deformability is not just a fundamentally interesting vesicle characteristic; it is also the key determinant of vesicle ability to cross the skin barrier; i.e. skin penetrability. Development of bilayer vesicles for drug and vaccine delivery across the skin should hence involve optimization of this property, which is controllable by the concentration of bilayer softeners in or near the vesicle bilayers. To this end, we propose a simple method for quantifying the effect of bilayer softeners on deformability of bilayer vesicles. The method derives the bending rigidity of vesicle bilayers from vesicle size dependence on softener concentration. To exemplify the method, we studied mixtures of soybean phosphatidylcholine with anionic sodium deoxycholate, non-ionic polyoxyethylene (20) sorbitan oleyl ester (polysorbate 80), or non-ionic polyoxyethylene (20) oleyl ether (C 18:1 EO 20 , Brij ® 98). With each of the tested bilayer softeners, the bending rigidity of the resulting mixed-amphipat vesicle bilayers decreased quasi-exponentially as the concentration of the bilayer softener increased, as one would expect on theoretical ground. The bilayer bending rigidity reached low values, near the thermal stability limit, i.e. k B T , before vesicle transformation into non-vesicular aggregates began. For a soybean phosphatidylcholine concentration of 5.0 mmol kg −1 , the bilayer bending rigidity reached 1.5 k B T at the total deoxycholate concentration of 4.1 mmol kg −1 and 3.4 k B T at the total polysorbate 80 concentration of 2.0 mmol kg −1 . In the case of C 18:1 EO 20 , the bilayer bending rigidity reached 1.5 k B T at the bilayer surface occupancy α = 0.1. The dependence of vesicle size on bilayer softener concentration thus reveals vesicle transformation into different aggregate structures (such as mixed micelles with poor skin penetrability) and practically valuable information on vesicle deformability. Our results compare favorably with results of literature measurements. We provide practical guidance on using the new analytical method to optimize deformable vesicle formulations. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Chemistry & Physics of Lipids is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.chemphyslip.2017.10.008 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 98 Subjects: – SubjectFull: Spectrum analysis Type: general – SubjectFull: Monomolecular films Type: general – SubjectFull: Deoxycholic acid Type: general – SubjectFull: Micelles synthesis Type: general – SubjectFull: Polyethylene glycol Type: general Titles: – TitleFull: The effect of membrane softeners on rigidity of lipid vesicle bilayers: Derivation from vesicle size changes. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Elsayed, Mustafa M.A. – PersonEntity: Name: NameFull: Ibrahim, Marwa M. – PersonEntity: Name: NameFull: Cevc, Gregor IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Text: Jan2018 Type: published Y: 2018 Identifiers: – Type: issn-print Value: 00093084 Numbering: – Type: volume Value: 210 Titles: – TitleFull: Chemistry & Physics of Lipids Type: main |
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