Mixture of Rectangular and Staggered Packing Arrangements of Polyalanine Region in Spider Dragline Silk in Dry and Hydrated States As Revealed by 13C NMR and X-ray Diffraction.
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| Title: | Mixture of Rectangular and Staggered Packing Arrangements of Polyalanine Region in Spider Dragline Silk in Dry and Hydrated States As Revealed by 13C NMR and X-ray Diffraction. |
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| Authors: | Tetsuo Asakura1 asakura@cc.tuat.ac.jp, Yugo Tasei1, Akihiro Aoki1, Akio Nishimura1 |
| Source: | Macromolecules. 2/13/2018, Vol. 51 Issue 3, p1058-1068. 11p. |
| Subjects: | Polyalanine, Draglines, Hydrates, Nuclear magnetic resonance spectroscopy, X-ray diffraction |
| Abstract: | For the first time, we determined the relative percentages of "rectangular" and "staggered" packing arrangements in the crystalline polyalanine regions with antiparallel β-sheet structure within spider dragline silk fiber from Nephila clavata (NCF) and recombinant silk protein (RSP). The methods used included X-ray diffraction and 13C NMR coupled with selective 13C isotope labeling of the Ala Cβ carbons. From deconvolution analyses of the Ala Cβ peaks in the 13C CP/MAS NMR spectra, the relative percentages of the rectangular arrangements in [3-13C]Ala-NCF were determined to be 49 ± 8% and 40 ± 7% in the dry and hydrated states, respectively, and in [3-13C]Ala-RSP 62 ± 11% and 81 ± 5% in the dry and hydrated states, respectively. Thus, the packing structure changed significantly between the two spider silks and also between the two physical states. The use of NMR was critical in this analysis; from X-ray diffraction patterns alone it would have been difficult to obtain these quantitative data. [ABSTRACT FROM AUTHOR] |
| Copyright of Macromolecules is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Items | – Name: Title Label: Title Group: Ti Data: Mixture of Rectangular and Staggered Packing Arrangements of Polyalanine Region in Spider Dragline Silk in Dry and Hydrated States As Revealed by <superscript>13</superscript>C NMR and X-ray Diffraction. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Tetsuo+Asakura%22">Tetsuo Asakura</searchLink><relatesTo>1</relatesTo><i> asakura@cc.tuat.ac.jp</i><br /><searchLink fieldCode="AR" term="%22Yugo+Tasei%22">Yugo Tasei</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Akihiro+Aoki%22">Akihiro Aoki</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Akio+Nishimura%22">Akio Nishimura</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Macromolecules%22">Macromolecules</searchLink>. 2/13/2018, Vol. 51 Issue 3, p1058-1068. 11p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Polyalanine%22">Polyalanine</searchLink><br /><searchLink fieldCode="DE" term="%22Draglines%22">Draglines</searchLink><br /><searchLink fieldCode="DE" term="%22Hydrates%22">Hydrates</searchLink><br /><searchLink fieldCode="DE" term="%22Nuclear+magnetic+resonance+spectroscopy%22">Nuclear magnetic resonance spectroscopy</searchLink><br /><searchLink fieldCode="DE" term="%22X-ray+diffraction%22">X-ray diffraction</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: For the first time, we determined the relative percentages of "rectangular" and "staggered" packing arrangements in the crystalline polyalanine regions with antiparallel β-sheet structure within spider dragline silk fiber from Nephila clavata (NCF) and recombinant silk protein (RSP). The methods used included X-ray diffraction and 13C NMR coupled with selective 13C isotope labeling of the Ala Cβ carbons. From deconvolution analyses of the Ala Cβ peaks in the 13C CP/MAS NMR spectra, the relative percentages of the rectangular arrangements in [3-13C]Ala-NCF were determined to be 49 ± 8% and 40 ± 7% in the dry and hydrated states, respectively, and in [3-13C]Ala-RSP 62 ± 11% and 81 ± 5% in the dry and hydrated states, respectively. Thus, the packing structure changed significantly between the two spider silks and also between the two physical states. The use of NMR was critical in this analysis; from X-ray diffraction patterns alone it would have been difficult to obtain these quantitative data. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Macromolecules is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1021/acs.macromol.7b02627 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 1058 Subjects: – SubjectFull: Polyalanine Type: general – SubjectFull: Draglines Type: general – SubjectFull: Hydrates Type: general – SubjectFull: Nuclear magnetic resonance spectroscopy Type: general – SubjectFull: X-ray diffraction Type: general Titles: – TitleFull: Mixture of Rectangular and Staggered Packing Arrangements of Polyalanine Region in Spider Dragline Silk in Dry and Hydrated States As Revealed by 13C NMR and X-ray Diffraction. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Tetsuo Asakura – PersonEntity: Name: NameFull: Yugo Tasei – PersonEntity: Name: NameFull: Akihiro Aoki – PersonEntity: Name: NameFull: Akio Nishimura IsPartOfRelationships: – BibEntity: Dates: – D: 13 M: 02 Text: 2/13/2018 Type: published Y: 2018 Identifiers: – Type: issn-print Value: 00249297 Numbering: – Type: volume Value: 51 – Type: issue Value: 3 Titles: – TitleFull: Macromolecules Type: main |
| ResultId | 1 |
