Coordinated regulation of intracellular pH by two glucose-sensing pathways in yeast.
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| Title: | Coordinated regulation of intracellular pH by two glucose-sensing pathways in yeast. |
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| Authors: | Isom, Daniel G.1,2 disom@miami.edu, Page, Stephani C.1, Collins, Leonard B.3, Kapolka, Nicholas J.2, Taghon, Geoffrey J.2, Dohlman, Henrik G.1 hdohlman@med.unc.edu |
| Source: | Journal of Biological Chemistry. 2/16/2018, Vol. 293 Issue 7, p2318-2329. 12p. |
| Subjects: | Glucose-regulated proteins, Saccharomyces cerevisiae, Cellular control mechanisms, Cellular signal transduction, Proton pump inhibitors, Cytoplasmic filaments, Fungi |
| Abstract: | The yeast Saccharomyces cerevisiae employs multiple pathways to coordinate sugar availability and metabolism. Glucose and other sugars are detected by a G protein-coupled receptor, Gpr1, as well as a pair of transporter-like proteins, Rgt2 and Snf3. When glucose is limiting, however, an ATP-driven proton pump (Pma1) is inactivated, leading to a marked decrease in cytoplasmic pH. Here we determine the relative contribution of the two sugar-sensing pathways to pH regulation. Whereas cytoplasmic pH is strongly dependent on glucose abundance and is regulated by both glucose-sensing pathways, ATP is largely unaffected and therefore cannot account for the changes in Pma1 activity. These data suggest that the pH is a second messenger of the glucose-sensing pathways. We show further that different sugars differ in their ability to control cellular acidification, in the manner of inverse agonists. We conclude that the sugar-sensing pathways act via Pma1 to invoke coordinated changes in cellularpHand metabolism. Morebroadly, our findings support the emerging view that cellular systems have evolved the use of pH signals as a means of adapting to environmental stresses such as those caused by hypoxia, ischemia, and diabetes. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Biological Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 128113120 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Coordinated regulation of intracellular pH by two glucose-sensing pathways in yeast. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Isom%2C+Daniel+G%2E%22">Isom, Daniel G.</searchLink><relatesTo>1,2</relatesTo><i> disom@miami.edu</i><br /><searchLink fieldCode="AR" term="%22Page%2C+Stephani+C%2E%22">Page, Stephani C.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Collins%2C+Leonard+B%2E%22">Collins, Leonard B.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Kapolka%2C+Nicholas+J%2E%22">Kapolka, Nicholas J.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Taghon%2C+Geoffrey+J%2E%22">Taghon, Geoffrey J.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Dohlman%2C+Henrik+G%2E%22">Dohlman, Henrik G.</searchLink><relatesTo>1</relatesTo><i> hdohlman@med.unc.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Biological+Chemistry%22">Journal of Biological Chemistry</searchLink>. 2/16/2018, Vol. 293 Issue 7, p2318-2329. 12p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Glucose-regulated+proteins%22">Glucose-regulated proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Saccharomyces+cerevisiae%22">Saccharomyces cerevisiae</searchLink><br /><searchLink fieldCode="DE" term="%22Cellular+control+mechanisms%22">Cellular control mechanisms</searchLink><br /><searchLink fieldCode="DE" term="%22Cellular+signal+transduction%22">Cellular signal transduction</searchLink><br /><searchLink fieldCode="DE" term="%22Proton+pump+inhibitors%22">Proton pump inhibitors</searchLink><br /><searchLink fieldCode="DE" term="%22Cytoplasmic+filaments%22">Cytoplasmic filaments</searchLink><br /><searchLink fieldCode="DE" term="%22Fungi%22">Fungi</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The yeast Saccharomyces cerevisiae employs multiple pathways to coordinate sugar availability and metabolism. Glucose and other sugars are detected by a G protein-coupled receptor, Gpr1, as well as a pair of transporter-like proteins, Rgt2 and Snf3. When glucose is limiting, however, an ATP-driven proton pump (Pma1) is inactivated, leading to a marked decrease in cytoplasmic pH. Here we determine the relative contribution of the two sugar-sensing pathways to pH regulation. Whereas cytoplasmic pH is strongly dependent on glucose abundance and is regulated by both glucose-sensing pathways, ATP is largely unaffected and therefore cannot account for the changes in Pma1 activity. These data suggest that the pH is a second messenger of the glucose-sensing pathways. We show further that different sugars differ in their ability to control cellular acidification, in the manner of inverse agonists. We conclude that the sugar-sensing pathways act via Pma1 to invoke coordinated changes in cellularpHand metabolism. Morebroadly, our findings support the emerging view that cellular systems have evolved the use of pH signals as a means of adapting to environmental stresses such as those caused by hypoxia, ischemia, and diabetes. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Biological Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1074/jbc.RA117.000422 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 12 StartPage: 2318 Subjects: – SubjectFull: Glucose-regulated proteins Type: general – SubjectFull: Saccharomyces cerevisiae Type: general – SubjectFull: Cellular control mechanisms Type: general – SubjectFull: Cellular signal transduction Type: general – SubjectFull: Proton pump inhibitors Type: general – SubjectFull: Cytoplasmic filaments Type: general – SubjectFull: Fungi Type: general Titles: – TitleFull: Coordinated regulation of intracellular pH by two glucose-sensing pathways in yeast. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Isom, Daniel G. – PersonEntity: Name: NameFull: Page, Stephani C. – PersonEntity: Name: NameFull: Collins, Leonard B. – PersonEntity: Name: NameFull: Kapolka, Nicholas J. – PersonEntity: Name: NameFull: Taghon, Geoffrey J. – PersonEntity: Name: NameFull: Dohlman, Henrik G. IsPartOfRelationships: – BibEntity: Dates: – D: 16 M: 02 Text: 2/16/2018 Type: published Y: 2018 Identifiers: – Type: issn-print Value: 00219258 Numbering: – Type: volume Value: 293 – Type: issue Value: 7 Titles: – TitleFull: Journal of Biological Chemistry Type: main |
| ResultId | 1 |
