View in EDS HTML Full Text PDF Full Text

Quantitative imaging of Candida utilis and its organelles by soft X‐ray Nano‐CT.

Saved in:
Bibliographic Details
Title: Quantitative imaging of Candida utilis and its organelles by soft X‐ray Nano‐CT.
Authors: LIU, J.1, LI, F.1, CHEN, L.1, GUAN, Y.1 yongg@ustc.edu.cn, TIAN, L.2, XIONG, Y.1, LIU, G.1, TIAN, Y.1
Source: Journal of Microscopy. Apr2018, Vol. 270 Issue 1, p64-70. 7p. 2 Color Photographs, 2 Black and White Photographs, 1 Diagram, 2 Charts, 2 Graphs.
Subjects: Candida utilis, Synchrotron radiation, Organelles, X-ray microscopy, Cytology
Abstract (English): Summary: Soft X‐ray microscopy has excellent characteristics for imaging cells and subcellular structures. In this paper, the yeast strain, Candida utilis, was imaged by soft X‐ray microscopy and three‐dimensional volumes were reconstructed with the SART‐TV method. We performed segmentation on the reconstruction in three dimensions and identified several types of subcellular architecture within the specimen cells based on their linear absorption coefficient (LAC) values. Organelles can be identified by the correlation between the soft X‐ray LAC values and the subcellular architectures. Quantitative analyses of the volume ratio of organelles to whole cell in different phases were also carried out according to the three‐dimensional datasets. With such excellent features, soft X‐ray imaging has a great influence in the field of biological cellular and subcellular research. [ABSTRACT FROM AUTHOR]
Abstract (Portuguese): Lay description: To observe and understand subcellular structures of cells, cryo soft X‐ray imaging is a promising and effective method. For identification of organelles inside the cell, different auxiliary imaging modalities are usually required, such as fluorescence microscopy, which will increase work and introduce toxicity to biological sample. In this work, absorption properties of soft X‐rays was used to identify the organelles inside the cell, because the absorption of soft X‐ray is an inherent property of substances. Quantitative analyses of the identified organelles of different cells were also carried out. The specific process methods as well as the experimental conditions are described in detail. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Microscopy is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
Full text is not displayed to guests.
FullText Links:
  – Type: pdflink
Text:
  Availability: 1
Header DbId: egs
DbLabel: Engineering Source
An: 128531778
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Quantitative imaging of <italic>Candida utilis</italic> and its organelles by soft X‐ray Nano‐CT.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22LIU%2C+J%2E%22">LIU, J.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22LI%2C+F%2E%22">LI, F.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22CHEN%2C+L%2E%22">CHEN, L.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22GUAN%2C+Y%2E%22">GUAN, Y.</searchLink><relatesTo>1</relatesTo><i> yongg@ustc.edu.cn</i><br /><searchLink fieldCode="AR" term="%22TIAN%2C+L%2E%22">TIAN, L.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22XIONG%2C+Y%2E%22">XIONG, Y.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22LIU%2C+G%2E%22">LIU, G.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22TIAN%2C+Y%2E%22">TIAN, Y.</searchLink><relatesTo>1</relatesTo>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Journal+of+Microscopy%22">Journal of Microscopy</searchLink>. Apr2018, Vol. 270 Issue 1, p64-70. 7p. 2 Color Photographs, 2 Black and White Photographs, 1 Diagram, 2 Charts, 2 Graphs.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Candida+utilis%22">Candida utilis</searchLink><br /><searchLink fieldCode="DE" term="%22Synchrotron+radiation%22">Synchrotron radiation</searchLink><br /><searchLink fieldCode="DE" term="%22Organelles%22">Organelles</searchLink><br /><searchLink fieldCode="DE" term="%22X-ray+microscopy%22">X-ray microscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Cytology%22">Cytology</searchLink>
– Name: Abstract
  Label: Abstract (English)
  Group: Ab
  Data: Summary: Soft X‐ray microscopy has excellent characteristics for imaging cells and subcellular structures. In this paper, the yeast strain, <italic>Candida utilis</italic>, was imaged by soft X‐ray microscopy and three‐dimensional volumes were reconstructed with the SART‐TV method. We performed segmentation on the reconstruction in three dimensions and identified several types of subcellular architecture within the specimen cells based on their linear absorption coefficient (LAC) values. Organelles can be identified by the correlation between the soft X‐ray LAC values and the subcellular architectures. Quantitative analyses of the volume ratio of organelles to whole cell in different phases were also carried out according to the three‐dimensional datasets. With such excellent features, soft X‐ray imaging has a great influence in the field of biological cellular and subcellular research. [ABSTRACT FROM AUTHOR]
– Name: Abstract
  Label: Abstract (Portuguese)
  Group: Ab
  Data: Lay description: To observe and understand subcellular structures of cells, cryo soft X‐ray imaging is a promising and effective method. For identification of organelles inside the cell, different auxiliary imaging modalities are usually required, such as fluorescence microscopy, which will increase work and introduce toxicity to biological sample. In this work, absorption properties of soft X‐rays was used to identify the organelles inside the cell, because the absorption of soft X‐ray is an inherent property of substances. Quantitative analyses of the identified organelles of different cells were also carried out. The specific process methods as well as the experimental conditions are described in detail. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Microscopy is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=128531778
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1111/jmi.12650
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 7
        StartPage: 64
    Subjects:
      – SubjectFull: Candida utilis
        Type: general
      – SubjectFull: Synchrotron radiation
        Type: general
      – SubjectFull: Organelles
        Type: general
      – SubjectFull: X-ray microscopy
        Type: general
      – SubjectFull: Cytology
        Type: general
    Titles:
      – TitleFull: Quantitative imaging of <italic>Candida utilis</italic> and its organelles by soft X‐ray Nano‐CT.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: LIU, J.
      – PersonEntity:
          Name:
            NameFull: LI, F.
      – PersonEntity:
          Name:
            NameFull: CHEN, L.
      – PersonEntity:
          Name:
            NameFull: GUAN, Y.
      – PersonEntity:
          Name:
            NameFull: TIAN, L.
      – PersonEntity:
          Name:
            NameFull: XIONG, Y.
      – PersonEntity:
          Name:
            NameFull: LIU, G.
      – PersonEntity:
          Name:
            NameFull: TIAN, Y.
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 04
              Text: Apr2018
              Type: published
              Y: 2018
          Identifiers:
            – Type: issn-print
              Value: 00222720
          Numbering:
            – Type: volume
              Value: 270
            – Type: issue
              Value: 1
          Titles:
            – TitleFull: Journal of Microscopy
              Type: main
ResultId 1