Heterologous expression, characterization and application of a new β-xylosidase identified in solventogenic Clostridium sp. strain BOH3.
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| Title: | Heterologous expression, characterization and application of a new β-xylosidase identified in solventogenic Clostridium sp. strain BOH3. |
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| Authors: | Li, Tinggang1, Wu, Yi-Rui1, He, Jianzhong1 jianzhong.he@nus.edu.sg |
| Source: | Process Biochemistry. Apr2018, Vol. 67, p99-104. 6p. |
| Subjects: | Xylosidases, Clostridium, Gene expression, Glycosidases, Hydrolysis, Escherichia coli |
| Abstract: | A β-xylosidase gene (Bxyl BOH3 ) was identified in a solventogenic bacterium Clostridium sp. strain BOH3, which is a member of the glycoside hydrolase family 43 (GH43). The recombinant enzyme of Bxyl BOH3 was expressed in Escherichia coli with a high specific activity of 48.7 U/mg and a low K m value of 2.71 mM at an optimal temperature of 40 °C and pH of 5.0-6.0, indicating its high affinity for the substrate and enzymatic potency. Moreover, activity of β-xylosidase can be enhanced (>1.6 times) by addition of Zn 2+ . The concentration of reducing sugar (including xylose and xylooligosaccharides) with the synergism of xylanase and recombinant β-xylosidase was enhanced up to 129.8% (11.93 g/L) after hydrolysis of 30 g/L of xylan for 24 h when comparing with only xylanase (9.19 g/L). Application of this recombinant β-xylosidase together with xylanase improved xylan hydrolysis efficiency, thus leading to increased biofuels productivity from xylan fermented by Clostridium species. [ABSTRACT FROM AUTHOR] |
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| Database: | Engineering Source |
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