Stable isotope labeling - dispersive solid phase extraction - liquid chromatography - tandem mass spectrometry for quantitative analysis of transsulfuration pathway thiols in human serum.
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| Title: | Stable isotope labeling - dispersive solid phase extraction - liquid chromatography - tandem mass spectrometry for quantitative analysis of transsulfuration pathway thiols in human serum. |
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| Authors: | Wang, Ya-Lan1, Zhu, Quan-Fei1, Cheng, Li-Ming2, Wang, Shao-Ting3, Qin, Shan-Shan4, Zheng, Shu-Jian1, Xiao, Hua-Ming1, Li, Juan-Juan3, Liu, Song-Mei4, Yuan, Bi-Feng1, Feng, Yu-Qi1 yqfeng@whu.edu.cn |
| Source: | Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences. Apr2018, Vol. 1083, p12-19. 8p. |
| Subjects: | Stable isotopes, Solid phase extraction, Liquid chromatography, Tandem mass spectrometry, Quantitative research, Thiols, Mass spectrometry |
| Abstract: | Low-molecular-weight thiols play important roles in a variety of pathological processes and are closely associated with a wide range of diseases. In this study, a selective and sensitive method was developed for the simultaneous determination of all the 7 thiols occurring in the transsulfuration pathway (Cysteine (Cys), homocysteine (Hcys), glutathione (GSH), N -acetylcysteine (Nac), cysteinylglycine (CysGly), glutamylcysteine (GluCys) and cysteamine (CA)) in human serum by in - vitro stable isotope labeling - dispersive solid phase extraction - liquid chromatography - tandem mass spectrometry analysis (IL-DSPE-LC-MS/MS). In the proposed method, a pair of stable isotope-labeling reagents, BQB ( ω -bromoacetonylquinolinium bromide) and BQB- D 7 , were utilized to label thiols in human serum samples and thiol standards, respectively. The BQB labeled thiols which carry a positive charge were extracted and purified with C 8 -SO 3 H-based DSPE followed by LC-MS/MS analysis. Good linearities for 7 thiols occurring in the transsulfuration pathway were obtained with the coefficient of determination ( R 2 ) >0.9901. The limits of detection (LODs) were in the range of 0.7–6.0 nmol/L. The method was further applied to investigate the contents change of 7 thiols in human serum samples of type 2 diabetes mellitus (T2DM) patients and breast cancer (BC) patients. The results showed that the contents of these thiols occurring in the transsulfuration pathway significantly changed and were highly diseases-related. In addition, partial least squares discriminant analysis (PLS-DA) suggested excellent classification performance between patients and healthy controls. The findings indicated that these significantly changed thiols occurring in the transsulfuration pathway in T2DM patients and BC patients might serve as the indicator for the diagnosis of T2DM and BC. Taken together, the developed IL-DSPE-LC-MS/MS method provides a promising tool for the sensitive analysis of thiols from complex biological samples, which may promote the in-depth investigation of the functions of thiols. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 128742710 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Stable isotope labeling - dispersive solid phase extraction - liquid chromatography - tandem mass spectrometry for quantitative analysis of transsulfuration pathway thiols in human serum. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Wang%2C+Ya-Lan%22">Wang, Ya-Lan</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Zhu%2C+Quan-Fei%22">Zhu, Quan-Fei</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Cheng%2C+Li-Ming%22">Cheng, Li-Ming</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Wang%2C+Shao-Ting%22">Wang, Shao-Ting</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Qin%2C+Shan-Shan%22">Qin, Shan-Shan</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Zheng%2C+Shu-Jian%22">Zheng, Shu-Jian</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Xiao%2C+Hua-Ming%22">Xiao, Hua-Ming</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Li%2C+Juan-Juan%22">Li, Juan-Juan</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Liu%2C+Song-Mei%22">Liu, Song-Mei</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Yuan%2C+Bi-Feng%22">Yuan, Bi-Feng</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Feng%2C+Yu-Qi%22">Feng, Yu-Qi</searchLink><relatesTo>1</relatesTo><i> yqfeng@whu.edu.cn</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Chromatography+B%3A+Analytical+Technologies+in+the+Biomedical+%26+Life+Sciences%22">Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences</searchLink>. Apr2018, Vol. 1083, p12-19. 8p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Stable+isotopes%22">Stable isotopes</searchLink><br /><searchLink fieldCode="DE" term="%22Solid+phase+extraction%22">Solid phase extraction</searchLink><br /><searchLink fieldCode="DE" term="%22Liquid+chromatography%22">Liquid chromatography</searchLink><br /><searchLink fieldCode="DE" term="%22Tandem+mass+spectrometry%22">Tandem mass spectrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Quantitative+research%22">Quantitative research</searchLink><br /><searchLink fieldCode="DE" term="%22Thiols%22">Thiols</searchLink><br /><searchLink fieldCode="DE" term="%22Mass+spectrometry%22">Mass spectrometry</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Low-molecular-weight thiols play important roles in a variety of pathological processes and are closely associated with a wide range of diseases. In this study, a selective and sensitive method was developed for the simultaneous determination of all the 7 thiols occurring in the transsulfuration pathway (Cysteine (Cys), homocysteine (Hcys), glutathione (GSH), N -acetylcysteine (Nac), cysteinylglycine (CysGly), glutamylcysteine (GluCys) and cysteamine (CA)) in human serum by in - vitro stable isotope labeling - dispersive solid phase extraction - liquid chromatography - tandem mass spectrometry analysis (IL-DSPE-LC-MS/MS). In the proposed method, a pair of stable isotope-labeling reagents, BQB ( ω -bromoacetonylquinolinium bromide) and BQB- D 7 , were utilized to label thiols in human serum samples and thiol standards, respectively. The BQB labeled thiols which carry a positive charge were extracted and purified with C 8 -SO 3 H-based DSPE followed by LC-MS/MS analysis. Good linearities for 7 thiols occurring in the transsulfuration pathway were obtained with the coefficient of determination ( R 2 ) >0.9901. The limits of detection (LODs) were in the range of 0.7–6.0 nmol/L. The method was further applied to investigate the contents change of 7 thiols in human serum samples of type 2 diabetes mellitus (T2DM) patients and breast cancer (BC) patients. The results showed that the contents of these thiols occurring in the transsulfuration pathway significantly changed and were highly diseases-related. In addition, partial least squares discriminant analysis (PLS-DA) suggested excellent classification performance between patients and healthy controls. The findings indicated that these significantly changed thiols occurring in the transsulfuration pathway in T2DM patients and BC patients might serve as the indicator for the diagnosis of T2DM and BC. Taken together, the developed IL-DSPE-LC-MS/MS method provides a promising tool for the sensitive analysis of thiols from complex biological samples, which may promote the in-depth investigation of the functions of thiols. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.jchromb.2018.02.036 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 8 StartPage: 12 Subjects: – SubjectFull: Stable isotopes Type: general – SubjectFull: Solid phase extraction Type: general – SubjectFull: Liquid chromatography Type: general – SubjectFull: Tandem mass spectrometry Type: general – SubjectFull: Quantitative research Type: general – SubjectFull: Thiols Type: general – SubjectFull: Mass spectrometry Type: general Titles: – TitleFull: Stable isotope labeling - dispersive solid phase extraction - liquid chromatography - tandem mass spectrometry for quantitative analysis of transsulfuration pathway thiols in human serum. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Wang, Ya-Lan – PersonEntity: Name: NameFull: Zhu, Quan-Fei – PersonEntity: Name: NameFull: Cheng, Li-Ming – PersonEntity: Name: NameFull: Wang, Shao-Ting – PersonEntity: Name: NameFull: Qin, Shan-Shan – PersonEntity: Name: NameFull: Zheng, Shu-Jian – PersonEntity: Name: NameFull: Xiao, Hua-Ming – PersonEntity: Name: NameFull: Li, Juan-Juan – PersonEntity: Name: NameFull: Liu, Song-Mei – PersonEntity: Name: NameFull: Yuan, Bi-Feng – PersonEntity: Name: NameFull: Feng, Yu-Qi IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 04 Text: Apr2018 Type: published Y: 2018 Identifiers: – Type: issn-print Value: 15700232 Numbering: – Type: volume Value: 1083 Titles: – TitleFull: Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences Type: main |
| ResultId | 1 |
