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Long-term fluorescence lifetime imaging of a genetically encoded sensor for caspase-3 activity in mouse tumor xenografts.

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Title: Long-term fluorescence lifetime imaging of a genetically encoded sensor for caspase-3 activity in mouse tumor xenografts.
Authors: Zherdeva, Victoria1, Kazachkina, Natalia I.1, Shcheslavskiy, Vladislav2, Savitsky, Alexander P.1 apsavitsky@inbi.ras.ru
Source: Journal of Biomedical Optics. Mar2018, Vol. 23 Issue 3, p1-11. 11p.
Subjects: Caspase genetics, Xenografts, Paclitaxel, Fluorescence resonance energy transfer, Apoptosis
Abstract: Caspase-3 is known for its role in apoptosis and programmed cell death regulation. We detected caspase-3 activation in vivo in tumor xenografts via shift of mean fluorescence lifetimes of a caspase-3 sensor. We used the genetically encoded sensor TR23K based on the red fluorescent protein TagRFP and chromoprotein KFP linked by 23 amino acid residues (TagRFP-23-KFP) containing a specific caspase cleavage DEVD motif to monitor the activity of caspase-3 in tumor xenografts by means of fluorescence lifetime imaging-Forster resonance energy transfer. Apoptosis was induced by injection of paclitaxel for A549 lung adenocarcinoma and etoposide and cisplatin for HEp-2 pharynx adenocarcinoma. We observed a shift in lifetime distribution from 1.6 to 1.9 ns to 2.1 to 2.4 ns, which indicated the activation of caspase-3. Even within the same tumor, the lifetime varied presumably due to the tumor heterogeneity and the different depth of tumor invasion. Thus, processing time-resolved fluorescence images allows detection of both the cleaved and noncleaved states of the TR23K sensor in real-time mode during the course of several weeks noninvasively. This approach can be used in drug screening, facilitating the development of new anticancer agents as well as improvement of chemotherapy efficiency and its adaptation for personal treatment. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Biomedical Optics is the property of SPIE - International Society of Optical Engineering and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 129183560
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PubType: Academic Journal
PubTypeId: academicJournal
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  Data: Long-term fluorescence lifetime imaging of a genetically encoded sensor for caspase-3 activity in mouse tumor xenografts.
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  Data: <searchLink fieldCode="AR" term="%22Zherdeva%2C+Victoria%22">Zherdeva, Victoria</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Kazachkina%2C+Natalia+I%2E%22">Kazachkina, Natalia I.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Shcheslavskiy%2C+Vladislav%22">Shcheslavskiy, Vladislav</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Savitsky%2C+Alexander+P%2E%22">Savitsky, Alexander P.</searchLink><relatesTo>1</relatesTo><i> apsavitsky@inbi.ras.ru</i>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Biomedical+Optics%22">Journal of Biomedical Optics</searchLink>. Mar2018, Vol. 23 Issue 3, p1-11. 11p.
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  Data: <searchLink fieldCode="DE" term="%22Caspase+genetics%22">Caspase genetics</searchLink><br /><searchLink fieldCode="DE" term="%22Xenografts%22">Xenografts</searchLink><br /><searchLink fieldCode="DE" term="%22Paclitaxel%22">Paclitaxel</searchLink><br /><searchLink fieldCode="DE" term="%22Fluorescence+resonance+energy+transfer%22">Fluorescence resonance energy transfer</searchLink><br /><searchLink fieldCode="DE" term="%22Apoptosis%22">Apoptosis</searchLink>
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  Label: Abstract
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  Data: Caspase-3 is known for its role in apoptosis and programmed cell death regulation. We detected caspase-3 activation in vivo in tumor xenografts via shift of mean fluorescence lifetimes of a caspase-3 sensor. We used the genetically encoded sensor TR23K based on the red fluorescent protein TagRFP and chromoprotein KFP linked by 23 amino acid residues (TagRFP-23-KFP) containing a specific caspase cleavage DEVD motif to monitor the activity of caspase-3 in tumor xenografts by means of fluorescence lifetime imaging-Forster resonance energy transfer. Apoptosis was induced by injection of paclitaxel for A549 lung adenocarcinoma and etoposide and cisplatin for HEp-2 pharynx adenocarcinoma. We observed a shift in lifetime distribution from 1.6 to 1.9 ns to 2.1 to 2.4 ns, which indicated the activation of caspase-3. Even within the same tumor, the lifetime varied presumably due to the tumor heterogeneity and the different depth of tumor invasion. Thus, processing time-resolved fluorescence images allows detection of both the cleaved and noncleaved states of the TR23K sensor in real-time mode during the course of several weeks noninvasively. This approach can be used in drug screening, facilitating the development of new anticancer agents as well as improvement of chemotherapy efficiency and its adaptation for personal treatment. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Biomedical Optics is the property of SPIE - International Society of Optical Engineering and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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    Identifiers:
      – Type: doi
        Value: 10.1117/1.JBO.23.3.035002
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      – Code: eng
        Text: English
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        PageCount: 11
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      – SubjectFull: Caspase genetics
        Type: general
      – SubjectFull: Xenografts
        Type: general
      – SubjectFull: Paclitaxel
        Type: general
      – SubjectFull: Fluorescence resonance energy transfer
        Type: general
      – SubjectFull: Apoptosis
        Type: general
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      – TitleFull: Long-term fluorescence lifetime imaging of a genetically encoded sensor for caspase-3 activity in mouse tumor xenografts.
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            NameFull: Zherdeva, Victoria
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            NameFull: Kazachkina, Natalia I.
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            NameFull: Shcheslavskiy, Vladislav
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            NameFull: Savitsky, Alexander P.
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              M: 03
              Text: Mar2018
              Type: published
              Y: 2018
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