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A new approach to the old problem: Inner filter effect type I and II in fluorescence.

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Title: A new approach to the old problem: Inner filter effect type I and II in fluorescence.
Authors: Kasparek, Adam1 adam.kasparek@uwm.edu.pl, Smyk, Bogdan1
Source: Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy. Jun2018, Vol. 198, p297-303. 7p.
Subjects: Fluorescence spectroscopy, Cuvettes (Optical instrument), Fluorescence yield, Fluorimeter, Absorption
Abstract: The fluorescence technique is very popular and has been used in many fields of research. It is simple in its assumptions but not very easy to use. One of the main problems is the inner filter effect (IF) I and II which takes place in the cuvette. IF type I is permanently present, but IF type II occurs only when absorption and fluorescence spectra overlap. To avoid IF type I, absorbencies in the cuvette should be smaller than 0.05, which is however very difficult to obtain in many experiments. In this work we propose a new method to solve these problems in the case of a Cary Eclipse fluorimeter, having horizontally-oriented slits, based on old equations developed in the middle of the last century. This method can be applied for other instruments, even these with vertically-oriented beams, because we share scripts written in MATLAB and GRAMS/AI environment. Calculations in our method enable specifying beam geometry parameters in the cuvette, which is necessary to obtain the correct shape and fluorescence intensity of emission and excitation spectra. Such a specific fluorescence intensity dependence on absorbance can, in many cases, afford possibilities to determine the quantum yield (QY) using slopes of the straight-lines, which was demonstrated with the use of Tryptophan (Trp), Tyrosine (Tyr), and Rhodamine B (RhB) solutions. For example, assuming that QY = 0.14 for Tyr, the QY determined for RhB reached QY = 0.71 ± 0.05, although the measurement for Tyr and RhB was performed at a completely different spectral range. [ABSTRACT FROM AUTHOR]
Copyright of Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
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Header DbId: egs
DbLabel: Engineering Source
An: 129207450
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
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  Group: Ti
  Data: A new approach to the old problem: Inner filter effect type I and II in fluorescence.
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  Data: <searchLink fieldCode="AR" term="%22Kasparek%2C+Adam%22">Kasparek, Adam</searchLink><relatesTo>1</relatesTo><i> adam.kasparek@uwm.edu.pl</i><br /><searchLink fieldCode="AR" term="%22Smyk%2C+Bogdan%22">Smyk, Bogdan</searchLink><relatesTo>1</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Spectrochimica+Acta+Part+A%3A+Molecular+%26+Biomolecular+Spectroscopy%22">Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy</searchLink>. Jun2018, Vol. 198, p297-303. 7p.
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  Data: <searchLink fieldCode="DE" term="%22Fluorescence+spectroscopy%22">Fluorescence spectroscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Cuvettes+%28Optical+instrument%29%22">Cuvettes (Optical instrument)</searchLink><br /><searchLink fieldCode="DE" term="%22Fluorescence+yield%22">Fluorescence yield</searchLink><br /><searchLink fieldCode="DE" term="%22Fluorimeter%22">Fluorimeter</searchLink><br /><searchLink fieldCode="DE" term="%22Absorption%22">Absorption</searchLink>
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  Label: Abstract
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  Data: The fluorescence technique is very popular and has been used in many fields of research. It is simple in its assumptions but not very easy to use. One of the main problems is the inner filter effect (IF) I and II which takes place in the cuvette. IF type I is permanently present, but IF type II occurs only when absorption and fluorescence spectra overlap. To avoid IF type I, absorbencies in the cuvette should be smaller than 0.05, which is however very difficult to obtain in many experiments. In this work we propose a new method to solve these problems in the case of a Cary Eclipse fluorimeter, having horizontally-oriented slits, based on old equations developed in the middle of the last century. This method can be applied for other instruments, even these with vertically-oriented beams, because we share scripts written in MATLAB and GRAMS/AI environment. Calculations in our method enable specifying beam geometry parameters in the cuvette, which is necessary to obtain the correct shape and fluorescence intensity of emission and excitation spectra. Such a specific fluorescence intensity dependence on absorbance can, in many cases, afford possibilities to determine the quantum yield (QY) using slopes of the straight-lines, which was demonstrated with the use of Tryptophan (Trp), Tyrosine (Tyr), and Rhodamine B (RhB) solutions. For example, assuming that QY = 0.14 for Tyr, the QY determined for RhB reached QY = 0.71 ± 0.05, although the measurement for Tyr and RhB was performed at a completely different spectral range. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=129207450
RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.saa.2018.03.027
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      – Code: eng
        Text: English
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        PageCount: 7
        StartPage: 297
    Subjects:
      – SubjectFull: Fluorescence spectroscopy
        Type: general
      – SubjectFull: Cuvettes (Optical instrument)
        Type: general
      – SubjectFull: Fluorescence yield
        Type: general
      – SubjectFull: Fluorimeter
        Type: general
      – SubjectFull: Absorption
        Type: general
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      – TitleFull: A new approach to the old problem: Inner filter effect type I and II in fluorescence.
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            NameFull: Kasparek, Adam
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            NameFull: Smyk, Bogdan
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            – D: 05
              M: 06
              Text: Jun2018
              Type: published
              Y: 2018
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              Value: 198
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            – TitleFull: Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy
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