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Evaluation of sheep ovarian tissue cryopreservation with slow freezing or vitrification after chick embryo chorioallantoic membrane transplantation.

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Title: Evaluation of sheep ovarian tissue cryopreservation with slow freezing or vitrification after chick embryo chorioallantoic membrane transplantation.
Authors: Vatanparast, Mahboubeh1,2, Khalili, Mohammad Ali1 Ma.khalili@ssu.ac.ir, Yari, Nahid1, Omidi, Marjan1, Mohsenzadeh, Mehdi1
Source: Cryobiology. Apr2018, Vol. 81, p178-184. 7p.
Subjects: Ovaries, Cryopreservation of organs, tissues, etc., Freezing, Chicken embryos, Chorioallantois, Vitrification, Transplantation of organs, tissues, etc.
Abstract: The aim of our investigations was to compare the effectiveness of two methods for cryopreservation of sheep ovarian tissue, slow freezing and vitrification. The quality of cryopreserved tissues was evaluated after 5 days of thawing and chorioallantoic membrane (CAM) transplantation. Follicular structure, stromal integrity and neovascularization were assessed. The areas of fibrosis and necrosis were measured using MICROVISIBLE software, and proliferation was assessed with Ki-67 immunostaning. After 5 days of culture, the proportion of primordial follicles decreased, whereas the primary and intermediary follicles increased insignificantly (p > .05). Only necrosis in the vitrified culture group increased significantly (p < .05). It was established also that 5 days CAM culture was not suitable methodology for detection of folliculogenesis. Follicular quality decreased after culture, but was better in fresh and slow frozen tissues than after vitrification (p < .05). Cellular proliferative activity fell, but it preserved to some extent in all groups. In conclusion, follicles was preserved better in grafted tissue after slow freezing than vitrification and stroma was more susceptible to ischemia in vitrified rather than conventional freezing in this view. Vitrification may not be a suitable alternative to the slow freezing. [ABSTRACT FROM AUTHOR]
Copyright of Cryobiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
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  Data: Evaluation of sheep ovarian tissue cryopreservation with slow freezing or vitrification after chick embryo chorioallantoic membrane transplantation.
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  Data: &lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Vatanparast%2C+Mahboubeh%22&quot;&gt;Vatanparast, Mahboubeh&lt;/searchLink&gt;&lt;relatesTo&gt;1,2&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Khalili%2C+Mohammad+Ali%22&quot;&gt;Khalili, Mohammad Ali&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt;&lt;i&gt; Ma.khalili@ssu.ac.ir&lt;/i&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Yari%2C+Nahid%22&quot;&gt;Yari, Nahid&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Omidi%2C+Marjan%22&quot;&gt;Omidi, Marjan&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Mohsenzadeh%2C+Mehdi%22&quot;&gt;Mohsenzadeh, Mehdi&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt;
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  Data: &lt;searchLink fieldCode=&quot;JN&quot; term=&quot;%22Cryobiology%22&quot;&gt;Cryobiology&lt;/searchLink&gt;. Apr2018, Vol. 81, p178-184. 7p.
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– Name: Abstract
  Label: Abstract
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  Data: The aim of our investigations was to compare the effectiveness of two methods for cryopreservation of sheep ovarian tissue, slow freezing and vitrification. The quality of cryopreserved tissues was evaluated after 5 days of thawing and chorioallantoic membrane (CAM) transplantation. Follicular structure, stromal integrity and neovascularization were assessed. The areas of fibrosis and necrosis were measured using MICROVISIBLE software, and proliferation was assessed with Ki-67 immunostaning. After 5 days of culture, the proportion of primordial follicles decreased, whereas the primary and intermediary follicles increased insignificantly (p &gt; .05). Only necrosis in the vitrified culture group increased significantly (p &lt; .05). It was established also that 5 days CAM culture was not suitable methodology for detection of folliculogenesis. Follicular quality decreased after culture, but was better in fresh and slow frozen tissues than after vitrification (p &lt; .05). Cellular proliferative activity fell, but it preserved to some extent in all groups. In conclusion, follicles was preserved better in grafted tissue after slow freezing than vitrification and stroma was more susceptible to ischemia in vitrified rather than conventional freezing in this view. Vitrification may not be a suitable alternative to the slow freezing. [ABSTRACT FROM AUTHOR]
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  Data: &lt;i&gt;Copyright of Cryobiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder&#39;s express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.&lt;/i&gt; (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.cryobiol.2018.01.002
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 7
        StartPage: 178
    Subjects:
      – SubjectFull: Ovaries
        Type: general
      – SubjectFull: Cryopreservation of organs, tissues, etc.
        Type: general
      – SubjectFull: Freezing
        Type: general
      – SubjectFull: Chicken embryos
        Type: general
      – SubjectFull: Chorioallantois
        Type: general
      – SubjectFull: Vitrification
        Type: general
      – SubjectFull: Transplantation of organs, tissues, etc.
        Type: general
    Titles:
      – TitleFull: Evaluation of sheep ovarian tissue cryopreservation with slow freezing or vitrification after chick embryo chorioallantoic membrane transplantation.
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      – PersonEntity:
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            NameFull: Vatanparast, Mahboubeh
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            NameFull: Khalili, Mohammad Ali
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            NameFull: Yari, Nahid
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            NameFull: Omidi, Marjan
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            NameFull: Mohsenzadeh, Mehdi
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          Dates:
            – D: 01
              M: 04
              Text: Apr2018
              Type: published
              Y: 2018
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              Value: 00112240
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              Value: 81
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            – TitleFull: Cryobiology
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