Systematic screening and characterization of Qi-Li-Qiang-Xin capsule-related xenobiotics in rats by ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry.
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| Title: | Systematic screening and characterization of Qi-Li-Qiang-Xin capsule-related xenobiotics in rats by ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry. |
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| Authors: | Yun, Wei-jing1, Yao, Zhi-hong2, Fan, Cai-lian1, Qin, Zi-fei2, Tang, Xi-yang2, Gao, Meng-xue2, Dai, Yi2 daiyi1004@163.com, Yao, Xin-sheng1,2 tyaoxs@jnu.edu.cn |
| Source: | Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences. Jul2018, Vol. 1090, p56-64. 9p. |
| Subjects: | Systematic Screening for Behavior Disorders, Xenobiotics, Liquid chromatography, Quadrupoles, Tandem mass spectrometry |
| Abstract: | Qi-Li-Qiang-Xin capsule (QLQX), a well-known traditional Chinese medicine prescription (TCMP), is consisted of eleven commonly used herbal medicines, has been widely used for the treatment of chronic heart failure (CHF). However, the absorbed components and related metabolites after oral administration of QLQX are still remaining unknown. In the present work, a reliable and effective method using ultra performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC/Q-TOF-MS) was established to identify QLQX-related xenobiotics in rats. Based on a representative structure based homologous xenobiotics identification (RSBHXI) strategy, a total of eleven compounds (salvianolic acid B, formononetin, benzoylmesaconine, alisol A, sinapine thiocyanate, naringin, tanshinone IIA, ginsenoside Rg1, ginsenoside Rb1, astragaloside IV and periplocin), bearing different chemical core structures, were selected and investigated for their metabolism in vivo . And then, comprehensive metabolic profiles of the holistic multi-ingredients in QLQX were achieved. As a result, a total of 121 QLQX-related xenobiotics (47 prototypes and 74 metabolites) were identified or tentatively characterized, among them eight prototypes (mesaconine, hypaconine, songorine, fuziline, neoline, talatizamine formononetin, neocryptotanshinone) and two metabolites (calycosin-gluA, formononetin-guA) were relatively the main existing xenobiotics exposed in blood. All absorbed prototype constituents were mainly from six composed herbal medicines (Aconiti lateralis radix, Astragali radix, Ginseng radix, Alismatis rhizoma, Salvia miltiorrhiza radix, Periploca cortex). The main metabolic reactions were methylation, hydrogenation, hydroxylation, oxidization, sulfation and glucuronidation. This is the first study on in vivo metabolism of QLQX. These results enabled us to focus on several high exposure ingredients in the discovery of effective substances of QLQX, however further pharmacokinetic study on these QLQX-related xenobiotics are needed to be carried out. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 129809197 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Systematic screening and characterization of Qi-Li-Qiang-Xin capsule-related xenobiotics in rats by ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Yun%2C+Wei-jing%22">Yun, Wei-jing</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Yao%2C+Zhi-hong%22">Yao, Zhi-hong</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Fan%2C+Cai-lian%22">Fan, Cai-lian</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Qin%2C+Zi-fei%22">Qin, Zi-fei</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Tang%2C+Xi-yang%22">Tang, Xi-yang</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Gao%2C+Meng-xue%22">Gao, Meng-xue</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Dai%2C+Yi%22">Dai, Yi</searchLink><relatesTo>2</relatesTo><i> daiyi1004@163.com</i><br /><searchLink fieldCode="AR" term="%22Yao%2C+Xin-sheng%22">Yao, Xin-sheng</searchLink><relatesTo>1,2</relatesTo><i> tyaoxs@jnu.edu.cn</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Chromatography+B%3A+Analytical+Technologies+in+the+Biomedical+%26+Life+Sciences%22">Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences</searchLink>. Jul2018, Vol. 1090, p56-64. 9p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Systematic+Screening+for+Behavior+Disorders%22">Systematic Screening for Behavior Disorders</searchLink><br /><searchLink fieldCode="DE" term="%22Xenobiotics%22">Xenobiotics</searchLink><br /><searchLink fieldCode="DE" term="%22Liquid+chromatography%22">Liquid chromatography</searchLink><br /><searchLink fieldCode="DE" term="%22Quadrupoles%22">Quadrupoles</searchLink><br /><searchLink fieldCode="DE" term="%22Tandem+mass+spectrometry%22">Tandem mass spectrometry</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Qi-Li-Qiang-Xin capsule (QLQX), a well-known traditional Chinese medicine prescription (TCMP), is consisted of eleven commonly used herbal medicines, has been widely used for the treatment of chronic heart failure (CHF). However, the absorbed components and related metabolites after oral administration of QLQX are still remaining unknown. In the present work, a reliable and effective method using ultra performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC/Q-TOF-MS) was established to identify QLQX-related xenobiotics in rats. Based on a representative structure based homologous xenobiotics identification (RSBHXI) strategy, a total of eleven compounds (salvianolic acid B, formononetin, benzoylmesaconine, alisol A, sinapine thiocyanate, naringin, tanshinone IIA, ginsenoside Rg1, ginsenoside Rb1, astragaloside IV and periplocin), bearing different chemical core structures, were selected and investigated for their metabolism in vivo . And then, comprehensive metabolic profiles of the holistic multi-ingredients in QLQX were achieved. As a result, a total of 121 QLQX-related xenobiotics (47 prototypes and 74 metabolites) were identified or tentatively characterized, among them eight prototypes (mesaconine, hypaconine, songorine, fuziline, neoline, talatizamine formononetin, neocryptotanshinone) and two metabolites (calycosin-gluA, formononetin-guA) were relatively the main existing xenobiotics exposed in blood. All absorbed prototype constituents were mainly from six composed herbal medicines (Aconiti lateralis radix, Astragali radix, Ginseng radix, Alismatis rhizoma, Salvia miltiorrhiza radix, Periploca cortex). The main metabolic reactions were methylation, hydrogenation, hydroxylation, oxidization, sulfation and glucuronidation. This is the first study on in vivo metabolism of QLQX. These results enabled us to focus on several high exposure ingredients in the discovery of effective substances of QLQX, however further pharmacokinetic study on these QLQX-related xenobiotics are needed to be carried out. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.jchromb.2018.05.014 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 56 Subjects: – SubjectFull: Systematic Screening for Behavior Disorders Type: general – SubjectFull: Xenobiotics Type: general – SubjectFull: Liquid chromatography Type: general – SubjectFull: Quadrupoles Type: general – SubjectFull: Tandem mass spectrometry Type: general Titles: – TitleFull: Systematic screening and characterization of Qi-Li-Qiang-Xin capsule-related xenobiotics in rats by ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Yun, Wei-jing – PersonEntity: Name: NameFull: Yao, Zhi-hong – PersonEntity: Name: NameFull: Fan, Cai-lian – PersonEntity: Name: NameFull: Qin, Zi-fei – PersonEntity: Name: NameFull: Tang, Xi-yang – PersonEntity: Name: NameFull: Gao, Meng-xue – PersonEntity: Name: NameFull: Dai, Yi – PersonEntity: Name: NameFull: Yao, Xin-sheng IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 07 Text: Jul2018 Type: published Y: 2018 Identifiers: – Type: issn-print Value: 15700232 Numbering: – Type: volume Value: 1090 Titles: – TitleFull: Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences Type: main |
| ResultId | 1 |
