Xanthophyllomyces dendrorhous physiological stages determination using combined measurements from dielectric and Raman spectroscopies, a cell counter system and fluorescence flow cytometry.
Saved in:
| Title: | Xanthophyllomyces dendrorhous physiological stages determination using combined measurements from dielectric and Raman spectroscopies, a cell counter system and fluorescence flow cytometry. |
|---|---|
| Authors: | Farías-Álvarez, L.J.1, Gschaedler-Mathis, A.1 agschaedler@ciatej.mx, Sánchez-Ortiz, A.F.1, Femat, R.2, Cervantes-Martínez, J.1, Arellano-Plaza, M.1, Zamora-Pedraza, C.1, Amillastre, E.1, Ghommidh, C.3, Herrera-López, E.J.1 eherrera@ciatej.mx |
| Source: | Biochemical Engineering Journal. Aug2018, Vol. 136, p1-8. 8p. |
| Subjects: | Xanthophyllomyces dendrorhous, Raman spectroscopy, Flow cytometry, Bioreactors, Fluorescence spectroscopy, Carotenoids |
| Abstract: | Dielectric spectroscopy, Raman spectroscopy, a cell counter system and fluorescence flow cytometry were used to monitor the yeast Xanthophyllomyces dendrorhous in bioreactor batch cultures. From the dielectric spectroscopy signal, it was possible to determine the maximum biomass growth, the viability of the cell and the time when the substrate was consumed. Raman spectroscopy allowed to measure intracellular carotenoids production. Cell vacuole metabolic activity was monitored from fluorescence flow cytometry and the cell counter allowed detect cell volume variability along the fermentation. The information obtained from the sensors measurements allowed us to suggest and characterize different physiological states of the yeast X. dendrorhous . [ABSTRACT FROM AUTHOR] |
| Copyright of Biochemical Engineering Journal is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
Be the first to leave a comment!
