Diffusion-based reverse membrane bioreactor for simultaneous bioconversion of high-inhibitor xylose-glucose media.
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| Title: | Diffusion-based reverse membrane bioreactor for simultaneous bioconversion of high-inhibitor xylose-glucose media. |
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| Authors: | Mahboubi, Amir1,2 amir.mahboubi_soufiani@hb.se, Lundin, Magnus1, Doyen, Wim2, De Wever, Heleen2, Taherzadeh, Mohammad J.1 |
| Source: | Process Biochemistry. Sep2018, Vol. 72, p23-30. 8p. |
| Subjects: | Lignocellulose, Detoxification (Alternative medicine), Xylose, Bioconversion, Bioreactors |
| Abstract: | Two of the main hurdles in industrial production of second generation bioethanol are the high content of inhibitory compounds and presence of sequentially fermented hexose and pentose saccharides in the feedstock. In order to tackle these issues, the novel cell confinement approach in a reverse membrane bioreactor (rMBR), used in this study, proved to be promising for robust fermentation of high-inhibitory xylose-glucose media simulating a lignocellulosic hydrolysate. The high local cell concentration and concentration-driven diffusion-based mass transfer conditions in rMBR enhanced simultaneous utilization of sugars and boosted cell furfural tolerance/detoxification capacity. The diffusion rates of all compounds through the membrane were measured in a diffusion cell and in an rMBR. In the rMBR, yeast cells could readily convert high content of furfural (10 g/l) that is toxic to freely-suspended cells. Moreover, in the presence of 2.5 g/l of furfural, cells had the same performance as in medium with no inhibitor and could simultaneously convert glucose, xylose, and furfural with the latter two at the same rate with no lag phase. The performance of rMBR in remediating issues revolving around lignocellulosic bioethanol production covers the shortcomings of the conventional encapsulation technique and opens new areas of application for diffusion-based bioconversion systems. [ABSTRACT FROM AUTHOR] |
| Copyright of Process Biochemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 131091610 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Diffusion-based reverse membrane bioreactor for simultaneous bioconversion of high-inhibitor xylose-glucose media. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Mahboubi%2C+Amir%22">Mahboubi, Amir</searchLink><relatesTo>1,2</relatesTo><i> amir.mahboubi_soufiani@hb.se</i><br /><searchLink fieldCode="AR" term="%22Lundin%2C+Magnus%22">Lundin, Magnus</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Doyen%2C+Wim%22">Doyen, Wim</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22De+Wever%2C+Heleen%22">De Wever, Heleen</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Taherzadeh%2C+Mohammad+J%2E%22">Taherzadeh, Mohammad J.</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Process+Biochemistry%22">Process Biochemistry</searchLink>. Sep2018, Vol. 72, p23-30. 8p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Lignocellulose%22">Lignocellulose</searchLink><br /><searchLink fieldCode="DE" term="%22Detoxification+%28Alternative+medicine%29%22">Detoxification (Alternative medicine)</searchLink><br /><searchLink fieldCode="DE" term="%22Xylose%22">Xylose</searchLink><br /><searchLink fieldCode="DE" term="%22Bioconversion%22">Bioconversion</searchLink><br /><searchLink fieldCode="DE" term="%22Bioreactors%22">Bioreactors</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Two of the main hurdles in industrial production of second generation bioethanol are the high content of inhibitory compounds and presence of sequentially fermented hexose and pentose saccharides in the feedstock. In order to tackle these issues, the novel cell confinement approach in a reverse membrane bioreactor (rMBR), used in this study, proved to be promising for robust fermentation of high-inhibitory xylose-glucose media simulating a lignocellulosic hydrolysate. The high local cell concentration and concentration-driven diffusion-based mass transfer conditions in rMBR enhanced simultaneous utilization of sugars and boosted cell furfural tolerance/detoxification capacity. The diffusion rates of all compounds through the membrane were measured in a diffusion cell and in an rMBR. In the rMBR, yeast cells could readily convert high content of furfural (10 g/l) that is toxic to freely-suspended cells. Moreover, in the presence of 2.5 g/l of furfural, cells had the same performance as in medium with no inhibitor and could simultaneously convert glucose, xylose, and furfural with the latter two at the same rate with no lag phase. The performance of rMBR in remediating issues revolving around lignocellulosic bioethanol production covers the shortcomings of the conventional encapsulation technique and opens new areas of application for diffusion-based bioconversion systems. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Process Biochemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.procbio.2018.06.007 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 8 StartPage: 23 Subjects: – SubjectFull: Lignocellulose Type: general – SubjectFull: Detoxification (Alternative medicine) Type: general – SubjectFull: Xylose Type: general – SubjectFull: Bioconversion Type: general – SubjectFull: Bioreactors Type: general Titles: – TitleFull: Diffusion-based reverse membrane bioreactor for simultaneous bioconversion of high-inhibitor xylose-glucose media. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Mahboubi, Amir – PersonEntity: Name: NameFull: Lundin, Magnus – PersonEntity: Name: NameFull: Doyen, Wim – PersonEntity: Name: NameFull: De Wever, Heleen – PersonEntity: Name: NameFull: Taherzadeh, Mohammad J. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 09 Text: Sep2018 Type: published Y: 2018 Identifiers: – Type: issn-print Value: 13595113 Numbering: – Type: volume Value: 72 Titles: – TitleFull: Process Biochemistry Type: main |
| ResultId | 1 |
