View in EDS

Selective monitoring of vascular cell senescence via β-Galactosidase detection with a fluorescent chemosensor.

Saved in:
Bibliographic Details
Title: Selective monitoring of vascular cell senescence via β-Galactosidase detection with a fluorescent chemosensor.
Authors: Kim, Eun-Joong1, Podder, Arup1,2, Maiti, Mrinmoy2, Lee, Jong Min3, Chung, Bong Geun1,3 bchung@sogang.ac.kr, Bhuniya, Sankarprasad1,2,4 b_sankarprasad@cb.amrita.edu
Source: Sensors & Actuators B: Chemical. Nov2018, Vol. 274, p194-200. 7p.
Subjects: Vascular endothelial cells, Cellular aging, Beta-galactosidase, Fluorescent probes, Lysosomes
Abstract: Graphical abstract Highlights • The probe SRP is nontoxic and biocompatible. • The probe SRP is highly selective toward β-galactosidase. • The probe SRP selectively is localized in lysosomes. • The probe SRP enables to detect vascular cell senescence. Abstract A new senescence-responsive fluorescent probe (SRP) was developed for the detection of β-galactosidase activity in senescent cells. UV-absorption of t probe SRP at 495 nm was increased in the presence of β-galactosidase. Its fluorescence at λ em 545 nm increased ∼27-fold upon incubation with β-galactosidase (0.1 U/mL). The SRP probe is non-toxic and highly chemoselective for β-galactosidase. The high cell viability and chemoselcivity of probe SRP offer it to be a suitable marker for assessing cell senescence in live cells. Using this probe, H 2 O 2 -induced cellular senescence of human umbilical vein cells (HUVECs) could be distinguished from normal cells based on the extent of fluorescent labeling of the cells. Moreover, it was preferentially localized in acidic lysosomes. Overall, SRP is a unique chemosensor that can provide preclinical information on cell senescence in vascular endothelial cells. [ABSTRACT FROM AUTHOR]
Copyright of Sensors & Actuators B: Chemical is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
Description
Abstract:Graphical abstract Highlights • The probe SRP is nontoxic and biocompatible. • The probe SRP is highly selective toward β-galactosidase. • The probe SRP selectively is localized in lysosomes. • The probe SRP enables to detect vascular cell senescence. Abstract A new senescence-responsive fluorescent probe (SRP) was developed for the detection of β-galactosidase activity in senescent cells. UV-absorption of t probe SRP at 495 nm was increased in the presence of β-galactosidase. Its fluorescence at λ em 545 nm increased ∼27-fold upon incubation with β-galactosidase (0.1 U/mL). The SRP probe is non-toxic and highly chemoselective for β-galactosidase. The high cell viability and chemoselcivity of probe SRP offer it to be a suitable marker for assessing cell senescence in live cells. Using this probe, H 2 O 2 -induced cellular senescence of human umbilical vein cells (HUVECs) could be distinguished from normal cells based on the extent of fluorescent labeling of the cells. Moreover, it was preferentially localized in acidic lysosomes. Overall, SRP is a unique chemosensor that can provide preclinical information on cell senescence in vascular endothelial cells. [ABSTRACT FROM AUTHOR]
ISSN:09254005
DOI:10.1016/j.snb.2018.07.171