2,3-Butanediol production by the non-pathogenic bacterium Paenibacillus brasilensis.
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| Title: | 2,3-Butanediol production by the non-pathogenic bacterium Paenibacillus brasilensis. |
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| Authors: | Dias, Beatriz do Carmo1, Lima, Maria Eduarda do Nascimento Vitorino2, Vollú, Renata Estebanez1, da Mota, Fabio Faria3, da Silva, Antonio Jorge Ribeiro4, de Castro, Aline Machado5, Freire, Denise Maria Guimarães2, Seldin, Lucy1 lseldin@micro.ufrj.br |
| Source: | Applied Microbiology & Biotechnology. Oct2018, Vol. 102 Issue 20, p8773-8782. 10p. |
| Subjects: | Paenibacillus, Butanediol, Dehydrogenases, Gas chromatography, Bacteria |
| Abstract: | 2,3-Butanediol (2,3-BDO) is of considerable importance in the chemical, plastic, pharmaceutical, cosmetic, and food industries. The main bacterial species producing this compound are considered pathogenic, hindering large-scale productivity. The species Paenibacillus brasilensis is generally recognized as safe (GRAS) and is phylogenetically similar to P. polymyxa, a species widely used for 2,3-BDO production. Here, we demonstrate, for the first time, that P. brasilensis strains produce 2,3-BDO. Total 2,3-BDO concentrations for 15 P. brasilensis strains varied from 5.5 to 7.6 g/l after 8 h incubation at 32 °C in modified YEPD medium containing 20 g/l glucose. Strain PB24 produced 8.2 g/l of 2,3-BDO within a 12-h growth period, representing a yield of 0.43 g/g and a productivity of 0.68 g/l/h. An increase in 2,3-BDO production by strain PB24 was observed using higher concentrations of glucose, reaching 27 g/l of total 2,3-BDO in YEPD containing about 80 g/l glucose within a 72-h growth period. We sequenced the genome of P. brasilensis PB24 and uncovered at least six genes related to the 2,3-BDO pathway at four distinct loci. We also compared gene sequences related to the 2,3-BDO pathway in P. brasilensis PB24 with those of other spore-forming bacteria, and found strong similarity to P. polymyxa, P. terrae, and P. peoriae 2,3-BDO-related genes. Regulatory regions upstream of these genes indicated that they are probably co-regulated. Finally, we propose a production pathway from glucose to 2,3-BDO in P. brasilensis PB24. Although the gene encoding S-2,3-butanediol dehydrogenase (butA) was found in the genome of P. brasilensis PB24, only R,R-2,3- and meso-2,3-butanediol were detected by gas chromatography under the growth conditions tested here. Our findings can serve as a basis for further improvements to the metabolic capabilities of this little-studied Paenibacillus species in relation to production of the high-value chemical 2,3-butanediol. [ABSTRACT FROM AUTHOR] |
| Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Header | DbId: egs DbLabel: Engineering Source An: 131976081 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: 2,3-Butanediol production by the non-pathogenic bacterium Paenibacillus brasilensis. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Dias%2C+Beatriz+do+Carmo%22">Dias, Beatriz do Carmo</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Lima%2C+Maria+Eduarda+do+Nascimento+Vitorino%22">Lima, Maria Eduarda do Nascimento Vitorino</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Vollú%2C+Renata+Estebanez%22">Vollú, Renata Estebanez</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22da+Mota%2C+Fabio+Faria%22">da Mota, Fabio Faria</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22da+Silva%2C+Antonio+Jorge+Ribeiro%22">da Silva, Antonio Jorge Ribeiro</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22de+Castro%2C+Aline+Machado%22">de Castro, Aline Machado</searchLink><relatesTo>5</relatesTo><br /><searchLink fieldCode="AR" term="%22Freire%2C+Denise+Maria+Guimarães%22">Freire, Denise Maria Guimarães</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Seldin%2C+Lucy%22">Seldin, Lucy</searchLink><relatesTo>1</relatesTo><i> lseldin@micro.ufrj.br</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Applied+Microbiology+%26+Biotechnology%22">Applied Microbiology & Biotechnology</searchLink>. Oct2018, Vol. 102 Issue 20, p8773-8782. 10p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Paenibacillus%22">Paenibacillus</searchLink><br /><searchLink fieldCode="DE" term="%22Butanediol%22">Butanediol</searchLink><br /><searchLink fieldCode="DE" term="%22Dehydrogenases%22">Dehydrogenases</searchLink><br /><searchLink fieldCode="DE" term="%22Gas+chromatography%22">Gas chromatography</searchLink><br /><searchLink fieldCode="DE" term="%22Bacteria%22">Bacteria</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: 2,3-Butanediol (2,3-BDO) is of considerable importance in the chemical, plastic, pharmaceutical, cosmetic, and food industries. The main bacterial species producing this compound are considered pathogenic, hindering large-scale productivity. The species Paenibacillus brasilensis is generally recognized as safe (GRAS) and is phylogenetically similar to P. polymyxa, a species widely used for 2,3-BDO production. Here, we demonstrate, for the first time, that P. brasilensis strains produce 2,3-BDO. Total 2,3-BDO concentrations for 15 P. brasilensis strains varied from 5.5 to 7.6 g/l after 8 h incubation at 32 °C in modified YEPD medium containing 20 g/l glucose. Strain PB24 produced 8.2 g/l of 2,3-BDO within a 12-h growth period, representing a yield of 0.43 g/g and a productivity of 0.68 g/l/h. An increase in 2,3-BDO production by strain PB24 was observed using higher concentrations of glucose, reaching 27 g/l of total 2,3-BDO in YEPD containing about 80 g/l glucose within a 72-h growth period. We sequenced the genome of P. brasilensis PB24 and uncovered at least six genes related to the 2,3-BDO pathway at four distinct loci. We also compared gene sequences related to the 2,3-BDO pathway in P. brasilensis PB24 with those of other spore-forming bacteria, and found strong similarity to P. polymyxa, P. terrae, and P. peoriae 2,3-BDO-related genes. Regulatory regions upstream of these genes indicated that they are probably co-regulated. Finally, we propose a production pathway from glucose to 2,3-BDO in P. brasilensis PB24. Although the gene encoding S-2,3-butanediol dehydrogenase (butA) was found in the genome of P. brasilensis PB24, only R,R-2,3- and meso-2,3-butanediol were detected by gas chromatography under the growth conditions tested here. Our findings can serve as a basis for further improvements to the metabolic capabilities of this little-studied Paenibacillus species in relation to production of the high-value chemical 2,3-butanediol. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=131976081 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00253-018-9312-y Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 10 StartPage: 8773 Subjects: – SubjectFull: Paenibacillus Type: general – SubjectFull: Butanediol Type: general – SubjectFull: Dehydrogenases Type: general – SubjectFull: Gas chromatography Type: general – SubjectFull: Bacteria Type: general Titles: – TitleFull: 2,3-Butanediol production by the non-pathogenic bacterium Paenibacillus brasilensis. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Dias, Beatriz do Carmo – PersonEntity: Name: NameFull: Lima, Maria Eduarda do Nascimento Vitorino – PersonEntity: Name: NameFull: Vollú, Renata Estebanez – PersonEntity: Name: NameFull: da Mota, Fabio Faria – PersonEntity: Name: NameFull: da Silva, Antonio Jorge Ribeiro – PersonEntity: Name: NameFull: de Castro, Aline Machado – PersonEntity: Name: NameFull: Freire, Denise Maria Guimarães – PersonEntity: Name: NameFull: Seldin, Lucy IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 10 Text: Oct2018 Type: published Y: 2018 Identifiers: – Type: issn-print Value: 01757598 Numbering: – Type: volume Value: 102 – Type: issue Value: 20 Titles: – TitleFull: Applied Microbiology & Biotechnology Type: main |
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