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Fabrication of dihydroxyflavone-conjugated hyaluronic acid nanogels for targeted antitumoral effect.

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Title: Fabrication of dihydroxyflavone-conjugated hyaluronic acid nanogels for targeted antitumoral effect.
Authors: Choi, Yu Ri1, Kim, Hyun-Jong1,2, Ahn, Guk Young1, Lee, Min Jeong1, Park, Ju Ri1, Jun, Dae-Ryong1, Ryu, Tae-Kyoung1, Park, Joo Woong3, Shin, Eunji3, Choi, Sung-Wook1 choisw@catholic.ac.kr
Source: Colloids & Surfaces B: Biointerfaces. Nov2018, Vol. 171, p690-697. 8p.
Subjects: Hyaluronic acid, Nanogels, Organic acids, Nuclear magnetic resonance spectroscopy, Liquid chromatography, Crosslinking (Polymerization), Polymerization
Abstract: Graphical abstract Highlights • Hyaluronic acid molecules are conjugated among themselves in nanogel form. • Dihydroxyflavones are conjugated to hyaluronic acid nanogels. • A low concentration of a conjugation agent leads to the nanogel size reduction. • The nanogel specifically reduced the viability of tumor cells to approximately 60%. Abstract We prepared hyaluronic acid (HA)-based nanogels conjugated with dihydroxyflavone (DHF) and evaluated their cellular uptake and antitumoral efficiency. 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) was used as a conjugation agent for esterification between DHF and HA as well as crosslinking among HA. The conjugations were confirmed by nuclear magnetic resonance spectroscopy, UV/vis spectroscopy, and high-performance liquid chromatography. The size and Zeta-potential of the DHF/HA nanogels were reduced with an increase in the concentration of DMTMM due to the involvement of more HA molecules for the conjugation reactions. The DHF/HA nanogel with a smaller size was greatly taken up by two kinds of tumor cells (HeLa and HepG2), compared to NIH3T3. The cell viabilities were reduced to approximately 60% for HeLa and HepG2 cells after 48 h post treatment with DHF/HA nanogels. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
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Abstract:Graphical abstract Highlights • Hyaluronic acid molecules are conjugated among themselves in nanogel form. • Dihydroxyflavones are conjugated to hyaluronic acid nanogels. • A low concentration of a conjugation agent leads to the nanogel size reduction. • The nanogel specifically reduced the viability of tumor cells to approximately 60%. Abstract We prepared hyaluronic acid (HA)-based nanogels conjugated with dihydroxyflavone (DHF) and evaluated their cellular uptake and antitumoral efficiency. 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) was used as a conjugation agent for esterification between DHF and HA as well as crosslinking among HA. The conjugations were confirmed by nuclear magnetic resonance spectroscopy, UV/vis spectroscopy, and high-performance liquid chromatography. The size and Zeta-potential of the DHF/HA nanogels were reduced with an increase in the concentration of DMTMM due to the involvement of more HA molecules for the conjugation reactions. The DHF/HA nanogel with a smaller size was greatly taken up by two kinds of tumor cells (HeLa and HepG2), compared to NIH3T3. The cell viabilities were reduced to approximately 60% for HeLa and HepG2 cells after 48 h post treatment with DHF/HA nanogels. [ABSTRACT FROM AUTHOR]
ISSN:09277765
DOI:10.1016/j.colsurfb.2018.08.003