The scale-up cultivation of Candida utilis in waste potato juice water with glycerol affects biomass and β(1,3)/(1,6)-glucan characteristic and yield.
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| Title: | The scale-up cultivation of Candida utilis in waste potato juice water with glycerol affects biomass and β(1,3)/(1,6)-glucan characteristic and yield. |
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| Authors: | Bzducha-Wróbel, Anna1 anna_bzducha_wrobel@sggw.pl, Pobiega, Katarzyna1, Błażejak, Stanisław1, Kieliszek, Marek1 |
| Source: | Applied Microbiology & Biotechnology. Nov2018, Vol. 102 Issue 21, p9131-9145. 15p. |
| Subjects: | Candida utilis, Glycerin, Glucans, Biomass, Osmotic pressure, Bacterial cell walls |
| Abstract: | New ideas on production of yeast origin β-glucan preparations for industrial application are attracting interest considering market development of that high-value functional polysaccharide. Sellecting an efficient yeast producer and designing culture conditions are a prerequisite for obtaining high yield of β-glucan. The aim of this study was to describe at the first time the influence of the mode of cultivation (shake-flasks and batch fermentation) and time of culture on characteristic and yield of biomass and β(1,3)/(1,6)-glucan preparations of Candida utilis ATCC 9950 after cultivation in medium based on waste potato juice water supplemented with 10% of glycerol. After shake-flask culture, the biomass was characterized by higher protein content (app. 26.5%) compared to 19% after batch fermentation while the cultivation on a biofermentor scale promoted polysaccharides biosynthesis. The highest output of purified β(1,3)/(1,6)-glucan preparation (5.3 gd.w./L), containing app. 85% of that polysaccharide, was found after 48 h cultivation in biofermentor. Batch fermentation promoted biosynthesis of alkali-insoluble β(1,3)/(1,6)-glucan fraction, decreasing the content of β(1,6)-glucan. The yield of β(1,3)/(1,6)-glucan synthesis was 0.063 (g/g glycerol), while the productivity of that polysaccharide reached 0.094 (g/L/h). Longer batch fermentation (72 h) resulted in reduction of production efficiency of β-glucan preparation under studied conditions. The results of the study provide a new efficient biotechnological solution to produce high-value β-glucan preparations of C. utilis origin based on valorization of agro-waste potato juice water with glycerol. [ABSTRACT FROM AUTHOR] |
| Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 132349041 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: The scale-up cultivation of Candida utilis in waste potato juice water with glycerol affects biomass and β(1,3)/(1,6)-glucan characteristic and yield. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Bzducha-Wróbel%2C+Anna%22">Bzducha-Wróbel, Anna</searchLink><relatesTo>1</relatesTo><i> anna_bzducha_wrobel@sggw.pl</i><br /><searchLink fieldCode="AR" term="%22Pobiega%2C+Katarzyna%22">Pobiega, Katarzyna</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Błażejak%2C+Stanisław%22">Błażejak, Stanisław</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Kieliszek%2C+Marek%22">Kieliszek, Marek</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Applied+Microbiology+%26+Biotechnology%22">Applied Microbiology & Biotechnology</searchLink>. Nov2018, Vol. 102 Issue 21, p9131-9145. 15p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Candida+utilis%22">Candida utilis</searchLink><br /><searchLink fieldCode="DE" term="%22Glycerin%22">Glycerin</searchLink><br /><searchLink fieldCode="DE" term="%22Glucans%22">Glucans</searchLink><br /><searchLink fieldCode="DE" term="%22Biomass%22">Biomass</searchLink><br /><searchLink fieldCode="DE" term="%22Osmotic+pressure%22">Osmotic pressure</searchLink><br /><searchLink fieldCode="DE" term="%22Bacterial+cell+walls%22">Bacterial cell walls</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: New ideas on production of yeast origin β-glucan preparations for industrial application are attracting interest considering market development of that high-value functional polysaccharide. Sellecting an efficient yeast producer and designing culture conditions are a prerequisite for obtaining high yield of β-glucan. The aim of this study was to describe at the first time the influence of the mode of cultivation (shake-flasks and batch fermentation) and time of culture on characteristic and yield of biomass and β(1,3)/(1,6)-glucan preparations of Candida utilis ATCC 9950 after cultivation in medium based on waste potato juice water supplemented with 10% of glycerol. After shake-flask culture, the biomass was characterized by higher protein content (app. 26.5%) compared to 19% after batch fermentation while the cultivation on a biofermentor scale promoted polysaccharides biosynthesis. The highest output of purified β(1,3)/(1,6)-glucan preparation (5.3 gd.w./L), containing app. 85% of that polysaccharide, was found after 48 h cultivation in biofermentor. Batch fermentation promoted biosynthesis of alkali-insoluble β(1,3)/(1,6)-glucan fraction, decreasing the content of β(1,6)-glucan. The yield of β(1,3)/(1,6)-glucan synthesis was 0.063 (g/g glycerol), while the productivity of that polysaccharide reached 0.094 (g/L/h). Longer batch fermentation (72 h) resulted in reduction of production efficiency of β-glucan preparation under studied conditions. The results of the study provide a new efficient biotechnological solution to produce high-value β-glucan preparations of C. utilis origin based on valorization of agro-waste potato juice water with glycerol. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00253-018-9357-y Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 15 StartPage: 9131 Subjects: – SubjectFull: Candida utilis Type: general – SubjectFull: Glycerin Type: general – SubjectFull: Glucans Type: general – SubjectFull: Biomass Type: general – SubjectFull: Osmotic pressure Type: general – SubjectFull: Bacterial cell walls Type: general Titles: – TitleFull: The scale-up cultivation of Candida utilis in waste potato juice water with glycerol affects biomass and β(1,3)/(1,6)-glucan characteristic and yield. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Bzducha-Wróbel, Anna – PersonEntity: Name: NameFull: Pobiega, Katarzyna – PersonEntity: Name: NameFull: Błażejak, Stanisław – PersonEntity: Name: NameFull: Kieliszek, Marek IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 11 Text: Nov2018 Type: published Y: 2018 Identifiers: – Type: issn-print Value: 01757598 Numbering: – Type: volume Value: 102 – Type: issue Value: 21 Titles: – TitleFull: Applied Microbiology & Biotechnology Type: main |
| ResultId | 1 |
