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Directed strain evolution restructures metabolism for 1-butanol production in minimal media.

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Title: Directed strain evolution restructures metabolism for 1-butanol production in minimal media.
Authors: Pontrelli, Sammy1, Fricke, Riley C.b.1, Sakurai, Sana Subhan Memon2, Putri, Sastia Prama2, Fitz-Gibbon, Sorel3, Chung, Matthew1, Wu, Hsin-Yi4, Chen, Yu-Ju5, Pellegrini, Matteo3,6, Fukusaki, Eiichiro2, Liao, James C.1,7 liaoj@gate.sinica.edu.tw
Source: Metabolic Engineering. Sep2018, Vol. 49, p153-163. 11p.
Subjects: Cell metabolism, Butanol, Integration host factor, Cell physiology, Pyruvate dehydrogenase complex
Abstract: Abstract Engineering a microbial strain for production sometimes entails metabolic modifications that impair essential physiological processes for growth or production. Restoring these functions may require amending a variety of non-obvious physiological networks, and thus, rational design strategies may not be practical. Here we demonstrate that growth and production may be restored by evolution that repairs impaired metabolic function. Furthermore, we use genomics, metabolomics and proteomics to identify several underlying mutations and metabolic perturbations that allow metabolism to repair. Previously, high titers of butanol production were achieved by Escherichia coli using a growth-coupled, modified Clostridial CoA-dependent pathway after all native fermentative pathways were deleted. However, production was only observed in rich media. Native metabolic function of the host was unable to support growth and production in minimal media. We use directed cell evolution to repair this phenotype and observed improved growth, titers and butanol yields. We found a mutation in pcnB which resulted in decreased plasmid copy numbers and pathway enzymes to balance resource utilization. Increased protein abundance was measured for biosynthetic pathways, glycolytic enzymes have increased activity, and adenosyl energy charge was increased. We also found mutations in the ArcAB two-component system and integration host factor (IHF) that tune redox metabolism to alter byproduct formation. These results demonstrate that directed strain evolution can enable systematic adaptations to repair metabolic function and enhance microbial production. Furthermore, these results demonstrate the versatile repair capabilities of cell metabolism and highlight important aspects of cell physiology that are required for production in minimal media. Highlights • Directed strain evolution restored metabolic function to enhance 1-butanol titers and yields in minimal media. • Mutation on pcnB decreased plasmid copy numbers and activity of pathway enzymes. • Glycolytic activity is increased, biosynthetic pathways are upregulated and metabolite degradation pathways are downregulated. • Mutations on arcB and ihfB are required in combination to increase expression of pyruvate dehydrogenase. [ABSTRACT FROM AUTHOR]
Copyright of Metabolic Engineering is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Directed strain evolution restructures metabolism for 1-butanol production in minimal media.
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  Data: <searchLink fieldCode="AR" term="%22Pontrelli%2C+Sammy%22">Pontrelli, Sammy</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Fricke%2C+Riley+C%2Eb%2E%22">Fricke, Riley C.b.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Sakurai%2C+Sana+Subhan+Memon%22">Sakurai, Sana Subhan Memon</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Putri%2C+Sastia+Prama%22">Putri, Sastia Prama</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Fitz-Gibbon%2C+Sorel%22">Fitz-Gibbon, Sorel</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Chung%2C+Matthew%22">Chung, Matthew</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Wu%2C+Hsin-Yi%22">Wu, Hsin-Yi</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Chen%2C+Yu-Ju%22">Chen, Yu-Ju</searchLink><relatesTo>5</relatesTo><br /><searchLink fieldCode="AR" term="%22Pellegrini%2C+Matteo%22">Pellegrini, Matteo</searchLink><relatesTo>3,6</relatesTo><br /><searchLink fieldCode="AR" term="%22Fukusaki%2C+Eiichiro%22">Fukusaki, Eiichiro</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Liao%2C+James+C%2E%22">Liao, James C.</searchLink><relatesTo>1,7</relatesTo><i> liaoj@gate.sinica.edu.tw</i>
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  Data: <searchLink fieldCode="DE" term="%22Cell+metabolism%22">Cell metabolism</searchLink><br /><searchLink fieldCode="DE" term="%22Butanol%22">Butanol</searchLink><br /><searchLink fieldCode="DE" term="%22Integration+host+factor%22">Integration host factor</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+physiology%22">Cell physiology</searchLink><br /><searchLink fieldCode="DE" term="%22Pyruvate+dehydrogenase+complex%22">Pyruvate dehydrogenase complex</searchLink>
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  Data: Abstract Engineering a microbial strain for production sometimes entails metabolic modifications that impair essential physiological processes for growth or production. Restoring these functions may require amending a variety of non-obvious physiological networks, and thus, rational design strategies may not be practical. Here we demonstrate that growth and production may be restored by evolution that repairs impaired metabolic function. Furthermore, we use genomics, metabolomics and proteomics to identify several underlying mutations and metabolic perturbations that allow metabolism to repair. Previously, high titers of butanol production were achieved by Escherichia coli using a growth-coupled, modified Clostridial CoA-dependent pathway after all native fermentative pathways were deleted. However, production was only observed in rich media. Native metabolic function of the host was unable to support growth and production in minimal media. We use directed cell evolution to repair this phenotype and observed improved growth, titers and butanol yields. We found a mutation in pcnB which resulted in decreased plasmid copy numbers and pathway enzymes to balance resource utilization. Increased protein abundance was measured for biosynthetic pathways, glycolytic enzymes have increased activity, and adenosyl energy charge was increased. We also found mutations in the ArcAB two-component system and integration host factor (IHF) that tune redox metabolism to alter byproduct formation. These results demonstrate that directed strain evolution can enable systematic adaptations to repair metabolic function and enhance microbial production. Furthermore, these results demonstrate the versatile repair capabilities of cell metabolism and highlight important aspects of cell physiology that are required for production in minimal media. Highlights • Directed strain evolution restored metabolic function to enhance 1-butanol titers and yields in minimal media. • Mutation on pcnB decreased plasmid copy numbers and activity of pathway enzymes. • Glycolytic activity is increased, biosynthetic pathways are upregulated and metabolite degradation pathways are downregulated. • Mutations on arcB and ihfB are required in combination to increase expression of pyruvate dehydrogenase. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Metabolic Engineering is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.ymben.2018.08.004
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        Text: English
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      – SubjectFull: Cell metabolism
        Type: general
      – SubjectFull: Butanol
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      – SubjectFull: Integration host factor
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      – SubjectFull: Cell physiology
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