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Thin layer chromatography combined with electrospray ionization mass spectrometry for characterizing herbal compounds.

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Title: Thin layer chromatography combined with electrospray ionization mass spectrometry for characterizing herbal compounds.
Authors: Cheng, Sy-Chyi1 (AUTHOR) m922020801@student.nsysu.edu.tw, Bhat, Suhail Muzaffar1 (AUTHOR), Lee, Chi-Wei2,3 (AUTHOR), Shiea, Jentaie1,4,5,6 (AUTHOR) jetea@mail.nsysu.edu.tw
Source: International Journal of Mass Spectrometry. Nov2018, Vol. 434, p264-271. 8p.
Subjects: Thin layer chromatography, Electrospray ionization mass spectrometry, Herbal medicine, Monkshoods, Chinese medicine, Phytochemicals, Ginseng
Abstract: Graphical abstract Highlights • Sawtooth TLC-ESI/MS was applied to characterize phytochemical compounds in medicinal herbs. • Highly toxic alkaloids were detected in Aconitum carmichaelii Debx. • Ginsenosides — the active ingredients in Panax ginseng — were detected. • Visualizable and non-visualizable analytes in TLC tracks were characterized. Abstract Characterizing the active ingredients and toxins in herbal products has important health implications in traditional Chinese medicine. Thin layer chromatography (TLC) is the most widely used technique for separating phytochemical compounds in plant extracts and has been coupled with mass spectrometry to provide sensitive and specific analysis. However, several analytical issues affect these methods, making them non-viable for long term and high-throughput analysis. We therefore developed a simple TLC/MS approach termed sawtooth thin layer chromatography-electrospray ionization/mass spectrometry (sawtooth TLC-ESI/MS) to directly scan and characterize herbal compounds on developed TLC plates that were modified into sawtooth pieces to facilitate rapid analysis. We directly induced electrospray ionization at each tip along the sawtooth edges to avoid detaching gel particles during analysis and to characterize both visualizable and non-visualizable analytes. Sawtooth TLC-ESI/MS was used to characterize the active ingredients and toxic compounds in Aconitum carmichaelii Debx and Panax ginseng root extracts. Sugar molecules and 16 alkaloids such as aconitine, mesaconitine, and hypaconitine were detected in the A. carmichaelii Debx extract, whereas 6 major ginsenosides were detected in the P. ginseng extract. [ABSTRACT FROM AUTHOR]
Copyright of International Journal of Mass Spectrometry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
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  Data: Thin layer chromatography combined with electrospray ionization mass spectrometry for characterizing herbal compounds.
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  Data: <searchLink fieldCode="AR" term="%22Cheng%2C+Sy-Chyi%22">Cheng, Sy-Chyi</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> m922020801@student.nsysu.edu.tw</i><br /><searchLink fieldCode="AR" term="%22Bhat%2C+Suhail+Muzaffar%22">Bhat, Suhail Muzaffar</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lee%2C+Chi-Wei%22">Lee, Chi-Wei</searchLink><relatesTo>2,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Shiea%2C+Jentaie%22">Shiea, Jentaie</searchLink><relatesTo>1,4,5,6</relatesTo> (AUTHOR)<i> jetea@mail.nsysu.edu.tw</i>
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  Data: <searchLink fieldCode="JN" term="%22International+Journal+of+Mass+Spectrometry%22">International Journal of Mass Spectrometry</searchLink>. Nov2018, Vol. 434, p264-271. 8p.
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  Data: <searchLink fieldCode="DE" term="%22Thin+layer+chromatography%22">Thin layer chromatography</searchLink><br /><searchLink fieldCode="DE" term="%22Electrospray+ionization+mass+spectrometry%22">Electrospray ionization mass spectrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Herbal+medicine%22">Herbal medicine</searchLink><br /><searchLink fieldCode="DE" term="%22Monkshoods%22">Monkshoods</searchLink><br /><searchLink fieldCode="DE" term="%22Chinese+medicine%22">Chinese medicine</searchLink><br /><searchLink fieldCode="DE" term="%22Phytochemicals%22">Phytochemicals</searchLink><br /><searchLink fieldCode="DE" term="%22Ginseng%22">Ginseng</searchLink>
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  Label: Abstract
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  Data: Graphical abstract Highlights • Sawtooth TLC-ESI/MS was applied to characterize phytochemical compounds in medicinal herbs. • Highly toxic alkaloids were detected in Aconitum carmichaelii Debx. • Ginsenosides — the active ingredients in Panax ginseng — were detected. • Visualizable and non-visualizable analytes in TLC tracks were characterized. Abstract Characterizing the active ingredients and toxins in herbal products has important health implications in traditional Chinese medicine. Thin layer chromatography (TLC) is the most widely used technique for separating phytochemical compounds in plant extracts and has been coupled with mass spectrometry to provide sensitive and specific analysis. However, several analytical issues affect these methods, making them non-viable for long term and high-throughput analysis. We therefore developed a simple TLC/MS approach termed sawtooth thin layer chromatography-electrospray ionization/mass spectrometry (sawtooth TLC-ESI/MS) to directly scan and characterize herbal compounds on developed TLC plates that were modified into sawtooth pieces to facilitate rapid analysis. We directly induced electrospray ionization at each tip along the sawtooth edges to avoid detaching gel particles during analysis and to characterize both visualizable and non-visualizable analytes. Sawtooth TLC-ESI/MS was used to characterize the active ingredients and toxic compounds in Aconitum carmichaelii Debx and Panax ginseng root extracts. Sugar molecules and 16 alkaloids such as aconitine, mesaconitine, and hypaconitine were detected in the A. carmichaelii Debx extract, whereas 6 major ginsenosides were detected in the P. ginseng extract. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
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  Data: <i>Copyright of International Journal of Mass Spectrometry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.ijms.2018.09.024
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      – Code: eng
        Text: English
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        PageCount: 8
        StartPage: 264
    Subjects:
      – SubjectFull: Thin layer chromatography
        Type: general
      – SubjectFull: Electrospray ionization mass spectrometry
        Type: general
      – SubjectFull: Herbal medicine
        Type: general
      – SubjectFull: Monkshoods
        Type: general
      – SubjectFull: Chinese medicine
        Type: general
      – SubjectFull: Phytochemicals
        Type: general
      – SubjectFull: Ginseng
        Type: general
    Titles:
      – TitleFull: Thin layer chromatography combined with electrospray ionization mass spectrometry for characterizing herbal compounds.
        Type: main
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          Name:
            NameFull: Cheng, Sy-Chyi
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            NameFull: Bhat, Suhail Muzaffar
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            NameFull: Lee, Chi-Wei
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            NameFull: Shiea, Jentaie
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            – D: 01
              M: 11
              Text: Nov2018
              Type: published
              Y: 2018
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              Value: 434
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            – TitleFull: International Journal of Mass Spectrometry
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