Application of Transcription Factor-based 3-Hydroxypropionic Acid Biosensor.
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| Title: | Application of Transcription Factor-based 3-Hydroxypropionic Acid Biosensor. |
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| Authors: | Nguyen, Nam Hoai1,2, Kim, Jung-Rae1, Park, Sunghoon1,2 parksh@unist.ac.kr |
| Source: | Biotechnology & Bioprocess Engineering. Sep2018, Vol. 23 Issue 5, p564-572. 9p. |
| Subjects: | Biosensors, Hydroxypropanone, Biochemical engineering, Transcription factors, Proteins |
| Abstract: | Application of the recently developed wholecell 3-HP biosensor, which detects 3-hydroxypropionic acid (3-HP) and expresses fluorescence as an output signal in response to the 3-HP concentration, was studied in three areas of enzyme and metabolic engineering. First, a sensor was applied to identify active 3-hydroxyaldehyde dehydrogenase (ALDH), a key enzyme in the 3-HP production pathway. Second, with the aid of helper enzymes that catalyze the conversion of 1,3-propanediol (1,3-PDO) to 3-HP, a 3-HP biosensor was converted into a 1,3-PDO biosensor. Third, a 3-HP biosensor, with proper modifications in expression module of the output signal and the use of pH-tolerant red fluorescent protein (RFP), was shown to monitor the rate of 3-HP production under process conditions in which one or more interfering compounds are present in the culture medium and/or the medium pH decreases. This study demonstrates that 3-HP biosensors can be widely used in enzyme and metabolic engineering applications for 3-HP production. [ABSTRACT FROM AUTHOR] |
| Copyright of Biotechnology & Bioprocess Engineering is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 132945262 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Application of Transcription Factor-based 3-Hydroxypropionic Acid Biosensor. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Nguyen%2C+Nam+Hoai%22">Nguyen, Nam Hoai</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Kim%2C+Jung-Rae%22">Kim, Jung-Rae</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Park%2C+Sunghoon%22">Park, Sunghoon</searchLink><relatesTo>1,2</relatesTo><i> parksh@unist.ac.kr</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Biotechnology+%26+Bioprocess+Engineering%22">Biotechnology & Bioprocess Engineering</searchLink>. Sep2018, Vol. 23 Issue 5, p564-572. 9p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Biosensors%22">Biosensors</searchLink><br /><searchLink fieldCode="DE" term="%22Hydroxypropanone%22">Hydroxypropanone</searchLink><br /><searchLink fieldCode="DE" term="%22Biochemical+engineering%22">Biochemical engineering</searchLink><br /><searchLink fieldCode="DE" term="%22Transcription+factors%22">Transcription factors</searchLink><br /><searchLink fieldCode="DE" term="%22Proteins%22">Proteins</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Application of the recently developed wholecell 3-HP biosensor, which detects 3-hydroxypropionic acid (3-HP) and expresses fluorescence as an output signal in response to the 3-HP concentration, was studied in three areas of enzyme and metabolic engineering. First, a sensor was applied to identify active 3-hydroxyaldehyde dehydrogenase (ALDH), a key enzyme in the 3-HP production pathway. Second, with the aid of helper enzymes that catalyze the conversion of 1,3-propanediol (1,3-PDO) to 3-HP, a 3-HP biosensor was converted into a 1,3-PDO biosensor. Third, a 3-HP biosensor, with proper modifications in expression module of the output signal and the use of pH-tolerant red fluorescent protein (RFP), was shown to monitor the rate of 3-HP production under process conditions in which one or more interfering compounds are present in the culture medium and/or the medium pH decreases. This study demonstrates that 3-HP biosensors can be widely used in enzyme and metabolic engineering applications for 3-HP production. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Biotechnology & Bioprocess Engineering is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s12257-018-0390-6 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 564 Subjects: – SubjectFull: Biosensors Type: general – SubjectFull: Hydroxypropanone Type: general – SubjectFull: Biochemical engineering Type: general – SubjectFull: Transcription factors Type: general – SubjectFull: Proteins Type: general Titles: – TitleFull: Application of Transcription Factor-based 3-Hydroxypropionic Acid Biosensor. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Nguyen, Nam Hoai – PersonEntity: Name: NameFull: Kim, Jung-Rae – PersonEntity: Name: NameFull: Park, Sunghoon IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 09 Text: Sep2018 Type: published Y: 2018 Identifiers: – Type: issn-print Value: 12268372 Numbering: – Type: volume Value: 23 – Type: issue Value: 5 Titles: – TitleFull: Biotechnology & Bioprocess Engineering Type: main |
| ResultId | 1 |
