View in EDS

Effects of hypoxia on gene and protein expression in the blue crab, Callinectes sapidus

Saved in:
Bibliographic Details
Title: Effects of hypoxia on gene and protein expression in the blue crab, Callinectes sapidus
Authors: Brouwer, Marius1 Marius.brouwer@usm.edu, Larkin, Patrick2,3, Brown-Peterson, Nancy1, King, Christina1, Manning, Steve1, Denslow, Nancy2
Source: Marine Environmental Research. Aug2004, Vol. 58 Issue 2-5, p787-792. 6p.
Subjects: Crabs, Hypoxia (Water), Biomarkers
Abstract: Increases in hypoxic conditions are one of the major factors responsible for declines in estuarine habitat quality, yet to date there are no indicators for recognizing populations of estuarine organisms that are suffering from chronic hypoxic stress. Here we test the hypothesis that alterations in gene and protein expression of antioxidant enzymes and other stress-specific proteins can be used as molecular indicators of hypoxic stress. Blue crabs, Callinectes sapidus, were exposed to 2–3 ppm DO for 5 days. Gene expression was measured using macroarrays constructed from cDNA of 10 partial gene transcripts cloned from blue crab hepatopancreas. Significant (p⩽0.05) down-regulation of gene expression was found for MnSOD, hemocyanin, ribosomal S15 and L23. Subtractive hybridization using RNA from control and hypoxic hepatopancreas tissues also indicated down-regulation of hemocyanin transcription. In contrast, Western blotting showed a significant (p⩽0.05) increase of hemocyanin protein in the hepatopancreas and cross-linking of MnSOD proteins in hypoxia-exposed crabs. Thus, hypoxia-responsive cDNA arrays and Westerns may be useful diagnostic tools for monitoring effects of hypoxia in estuarine crustacea. [Copyright &y& Elsevier]
Copyright of Marine Environmental Research is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 13333476
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Effects of hypoxia on gene and protein expression in the blue crab, Callinectes sapidus
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Brouwer%2C+Marius%22">Brouwer, Marius</searchLink><relatesTo>1</relatesTo><i> Marius.brouwer@usm.edu</i><br /><searchLink fieldCode="AR" term="%22Larkin%2C+Patrick%22">Larkin, Patrick</searchLink><relatesTo>2,3</relatesTo><br /><searchLink fieldCode="AR" term="%22Brown-Peterson%2C+Nancy%22">Brown-Peterson, Nancy</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22King%2C+Christina%22">King, Christina</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Manning%2C+Steve%22">Manning, Steve</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Denslow%2C+Nancy%22">Denslow, Nancy</searchLink><relatesTo>2</relatesTo>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Marine+Environmental+Research%22">Marine Environmental Research</searchLink>. Aug2004, Vol. 58 Issue 2-5, p787-792. 6p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Crabs%22">Crabs</searchLink><br /><searchLink fieldCode="DE" term="%22Hypoxia+%28Water%29%22">Hypoxia (Water)</searchLink><br /><searchLink fieldCode="DE" term="%22Biomarkers%22">Biomarkers</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Increases in hypoxic conditions are one of the major factors responsible for declines in estuarine habitat quality, yet to date there are no indicators for recognizing populations of estuarine organisms that are suffering from chronic hypoxic stress. Here we test the hypothesis that alterations in gene and protein expression of antioxidant enzymes and other stress-specific proteins can be used as molecular indicators of hypoxic stress. Blue crabs, Callinectes sapidus, were exposed to 2–3 ppm DO for 5 days. Gene expression was measured using macroarrays constructed from cDNA of 10 partial gene transcripts cloned from blue crab hepatopancreas. Significant (<f>p⩽0.05</f>) down-regulation of gene expression was found for MnSOD, hemocyanin, ribosomal S15 and L23. Subtractive hybridization using RNA from control and hypoxic hepatopancreas tissues also indicated down-regulation of hemocyanin transcription. In contrast, Western blotting showed a significant (<f>p⩽0.05</f>) increase of hemocyanin protein in the hepatopancreas and cross-linking of MnSOD proteins in hypoxia-exposed crabs. Thus, hypoxia-responsive cDNA arrays and Westerns may be useful diagnostic tools for monitoring effects of hypoxia in estuarine crustacea. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Marine Environmental Research is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=13333476
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.marenvres.2004.03.094
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 6
        StartPage: 787
    Subjects:
      – SubjectFull: Crabs
        Type: general
      – SubjectFull: Hypoxia (Water)
        Type: general
      – SubjectFull: Biomarkers
        Type: general
    Titles:
      – TitleFull: Effects of hypoxia on gene and protein expression in the blue crab, Callinectes sapidus
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Brouwer, Marius
      – PersonEntity:
          Name:
            NameFull: Larkin, Patrick
      – PersonEntity:
          Name:
            NameFull: Brown-Peterson, Nancy
      – PersonEntity:
          Name:
            NameFull: King, Christina
      – PersonEntity:
          Name:
            NameFull: Manning, Steve
      – PersonEntity:
          Name:
            NameFull: Denslow, Nancy
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 08
              Text: Aug2004
              Type: published
              Y: 2004
          Identifiers:
            – Type: issn-print
              Value: 01411136
          Numbering:
            – Type: volume
              Value: 58
            – Type: issue
              Value: 2-5
          Titles:
            – TitleFull: Marine Environmental Research
              Type: main
ResultId 1