Multiple combinations of RDL subunits diversify the repertoire of GABA receptors in the honey bee parasite Varroa destructor.
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| Title: | Multiple combinations of RDL subunits diversify the repertoire of GABA receptors in the honey bee parasite Varroa destructor. |
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| Authors: | Ménard, Claudine1, Folacci, Mathilde1, Brunello, Lorène1, Charreton, Mercedes2, Collet, Claude2, Mary, Rosanna1, Rousset, Matthieu1, Thibaud, Jean-Baptiste1, Vignes, Michel1, Charnet, Pierre1, Cens, Thierry1 thierry.cens@inserm.fr |
| Source: | Journal of Biological Chemistry. 12/7/2018, Vol. 293 Issue 49, p19012-19024. 13p. |
| Subjects: | Varroa destructor, Honeybees, Neurotransmitters, Cationic lipids, Ectoparasites |
| Abstract: | In insects, γ-aminobutyric acid (GABA) is the major inhibitory neurotransmitter, and GABA-gated ion channels are the target of different classes of insecticides, including fipronil. We report here the cloning of six subunits (four RDL, one LCCH3, and one GRD) that constitute the repertoire of the GABA-gated ion channel family of the Varroa mite (Varroa destructor), a honey bee ectoparasite. We also isolated a truncated GRD subunit with a premature stop codon. We found that when expressed in Xenopus laevis oocytes, three of the four RDL subunits (VdesRDL1, VdesRDL2, and VdesRDL3) formed functional, homomultimeric anionic receptors, whereas GRD and LCCH3 produced heteromultimeric cationic receptors. These receptors displayed specific sensitivities toward GABA and fipronil, and VdesRDL1 was the most resistant to the insecticide. We identified specific residues in the VdesRDL1 pore-lining region that explain its high resistance to fipronil. VdesRDL4 did not form a functional receptor when expressed alone, but it assembled with VdesRDL1 to form a heteromultimeric receptor with properties distinct from those of the VdesRDL1 homomultimeric receptor. Moreover, VdesRDL1 physically interacted with VdesRDL3, generating a heteromultimeric receptor combining properties of both subunits. On the other hand, we did not detect any functional interaction between VdesLCCH3 and the VdesRDL subunits, an observation that differed from what was previously reported for Drosophila melanogaster. In conclusion, this study provides insights relevant to improve our understanding of the precise role of GABAergic signaling in insects and new tools for the development of Varroa mite-specific insecticidal agents that do not harm honey bees. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Biological Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 133475451 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Multiple combinations of RDL subunits diversify the repertoire of GABA receptors in the honey bee parasite Varroa destructor. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Ménard%2C+Claudine%22">Ménard, Claudine</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Folacci%2C+Mathilde%22">Folacci, Mathilde</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Brunello%2C+Lorène%22">Brunello, Lorène</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Charreton%2C+Mercedes%22">Charreton, Mercedes</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Collet%2C+Claude%22">Collet, Claude</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Mary%2C+Rosanna%22">Mary, Rosanna</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Rousset%2C+Matthieu%22">Rousset, Matthieu</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Thibaud%2C+Jean-Baptiste%22">Thibaud, Jean-Baptiste</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Vignes%2C+Michel%22">Vignes, Michel</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Charnet%2C+Pierre%22">Charnet, Pierre</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Cens%2C+Thierry%22">Cens, Thierry</searchLink><relatesTo>1</relatesTo><i> thierry.cens@inserm.fr</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Biological+Chemistry%22">Journal of Biological Chemistry</searchLink>. 12/7/2018, Vol. 293 Issue 49, p19012-19024. 13p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Varroa+destructor%22">Varroa destructor</searchLink><br /><searchLink fieldCode="DE" term="%22Honeybees%22">Honeybees</searchLink><br /><searchLink fieldCode="DE" term="%22Neurotransmitters%22">Neurotransmitters</searchLink><br /><searchLink fieldCode="DE" term="%22Cationic+lipids%22">Cationic lipids</searchLink><br /><searchLink fieldCode="DE" term="%22Ectoparasites%22">Ectoparasites</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: In insects, γ-aminobutyric acid (GABA) is the major inhibitory neurotransmitter, and GABA-gated ion channels are the target of different classes of insecticides, including fipronil. We report here the cloning of six subunits (four RDL, one LCCH3, and one GRD) that constitute the repertoire of the GABA-gated ion channel family of the Varroa mite (Varroa destructor), a honey bee ectoparasite. We also isolated a truncated GRD subunit with a premature stop codon. We found that when expressed in Xenopus laevis oocytes, three of the four RDL subunits (VdesRDL1, VdesRDL2, and VdesRDL3) formed functional, homomultimeric anionic receptors, whereas GRD and LCCH3 produced heteromultimeric cationic receptors. These receptors displayed specific sensitivities toward GABA and fipronil, and VdesRDL1 was the most resistant to the insecticide. We identified specific residues in the VdesRDL1 pore-lining region that explain its high resistance to fipronil. VdesRDL4 did not form a functional receptor when expressed alone, but it assembled with VdesRDL1 to form a heteromultimeric receptor with properties distinct from those of the VdesRDL1 homomultimeric receptor. Moreover, VdesRDL1 physically interacted with VdesRDL3, generating a heteromultimeric receptor combining properties of both subunits. On the other hand, we did not detect any functional interaction between VdesLCCH3 and the VdesRDL subunits, an observation that differed from what was previously reported for Drosophila melanogaster. In conclusion, this study provides insights relevant to improve our understanding of the precise role of GABAergic signaling in insects and new tools for the development of Varroa mite-specific insecticidal agents that do not harm honey bees. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Biological Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1074/jbc.RA118.005365 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 13 StartPage: 19012 Subjects: – SubjectFull: Varroa destructor Type: general – SubjectFull: Honeybees Type: general – SubjectFull: Neurotransmitters Type: general – SubjectFull: Cationic lipids Type: general – SubjectFull: Ectoparasites Type: general Titles: – TitleFull: Multiple combinations of RDL subunits diversify the repertoire of GABA receptors in the honey bee parasite Varroa destructor. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Ménard, Claudine – PersonEntity: Name: NameFull: Folacci, Mathilde – PersonEntity: Name: NameFull: Brunello, Lorène – PersonEntity: Name: NameFull: Charreton, Mercedes – PersonEntity: Name: NameFull: Collet, Claude – PersonEntity: Name: NameFull: Mary, Rosanna – PersonEntity: Name: NameFull: Rousset, Matthieu – PersonEntity: Name: NameFull: Thibaud, Jean-Baptiste – PersonEntity: Name: NameFull: Vignes, Michel – PersonEntity: Name: NameFull: Charnet, Pierre – PersonEntity: Name: NameFull: Cens, Thierry IsPartOfRelationships: – BibEntity: Dates: – D: 07 M: 12 Text: 12/7/2018 Type: published Y: 2018 Identifiers: – Type: issn-print Value: 00219258 Numbering: – Type: volume Value: 293 – Type: issue Value: 49 Titles: – TitleFull: Journal of Biological Chemistry Type: main |
| ResultId | 1 |
