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Microbial biosynthesis and in vivo depolymerization of intracellular medium‐chain‐length poly‐3‐hydroxyalkanoates as potential route to platform chemicals.

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Title: Microbial biosynthesis and in vivo depolymerization of intracellular medium‐chain‐length poly‐3‐hydroxyalkanoates as potential route to platform chemicals.
Authors: Anis, Siti Nor Syairah1, Mohd Annuar, Mohd Suffian1, Simarani, Khanom1
Source: Biotechnology & Applied Biochemistry. Nov/Dec2018, Vol. 65 Issue 6, p784-796. 13p.
Subjects: Poly-beta-hydroxyalkanoates, Biosynthesis, Depolymerization, Pseudomonas putida, Lauric acid
Abstract: Biosynthesis and in vivo depolymerization of intracellular medium‐chain‐length poly‐3‐hydroxyalkanoates (mcl‐PHA) in Pseudomonas putida Bet001 grown on lauric acid were studied. Highest mcl‐PHA fraction (>50 % of total biomass) and cell concentration (8 g L−1) were obtained at carbon‐to‐nitrogen (C/N) ratio 20, starting cell concentration 1 g L−1, and 48 H fermentation. The mcl‐PHA comprised of 3‐hydroxyhexanoate (C6), 3‐hydroxyoctanote (C8), 3‐hydroxydecanoate (C10), and 3‐hydroxydodecanoate (C12) monomers. In vivo action was studied in a mineral liquid medium without carbon source, and in different buffer solutions with varied pH, molarity, ionic strength, and temperature. The monomer liberation rate reflected the mol percentage distribution of the initial polymer subunit composition. Rate and percentage of in vivo depolymerization were highest in 0.2 M Tris–HCl buffer (pH 9, strength = 0.2 M, 30 °C) at 0.21 g L−1 H−1 and 98.6 ± 1.3 wt%, respectively. There is a congruity vis‐à‐vis to specific buffer type, molarity, pH, ionic strength, and temperature values for superior in vivo depolymerization activities. Direct products from in vivo depolymerization matched the individual monomeric composition of native mcl‐PHA. It points to exo‐type reaction for the in vivo process, and potential biological route to chiral molecules. [ABSTRACT FROM AUTHOR]
Copyright of Biotechnology & Applied Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Microbial biosynthesis and in vivo depolymerization of intracellular medium‐chain‐length poly‐3‐hydroxyalkanoates as potential route to platform chemicals.
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  Data: <searchLink fieldCode="AR" term="%22Anis%2C+Siti+Nor+Syairah%22">Anis, Siti Nor Syairah</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Mohd+Annuar%2C+Mohd+Suffian%22">Mohd Annuar, Mohd Suffian</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Simarani%2C+Khanom%22">Simarani, Khanom</searchLink><relatesTo>1</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Biotechnology+%26+Applied+Biochemistry%22">Biotechnology & Applied Biochemistry</searchLink>. Nov/Dec2018, Vol. 65 Issue 6, p784-796. 13p.
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  Data: <searchLink fieldCode="DE" term="%22Poly-beta-hydroxyalkanoates%22">Poly-beta-hydroxyalkanoates</searchLink><br /><searchLink fieldCode="DE" term="%22Biosynthesis%22">Biosynthesis</searchLink><br /><searchLink fieldCode="DE" term="%22Depolymerization%22">Depolymerization</searchLink><br /><searchLink fieldCode="DE" term="%22Pseudomonas+putida%22">Pseudomonas putida</searchLink><br /><searchLink fieldCode="DE" term="%22Lauric+acid%22">Lauric acid</searchLink>
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  Label: Abstract
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  Data: Biosynthesis and in vivo depolymerization of intracellular medium‐chain‐length poly‐3‐hydroxyalkanoates (mcl‐PHA) in Pseudomonas putida Bet001 grown on lauric acid were studied. Highest mcl‐PHA fraction (>50 % of total biomass) and cell concentration (8 g L−1) were obtained at carbon‐to‐nitrogen (C/N) ratio 20, starting cell concentration 1 g L−1, and 48 H fermentation. The mcl‐PHA comprised of 3‐hydroxyhexanoate (C6), 3‐hydroxyoctanote (C8), 3‐hydroxydecanoate (C10), and 3‐hydroxydodecanoate (C12) monomers. In vivo action was studied in a mineral liquid medium without carbon source, and in different buffer solutions with varied pH, molarity, ionic strength, and temperature. The monomer liberation rate reflected the mol percentage distribution of the initial polymer subunit composition. Rate and percentage of in vivo depolymerization were highest in 0.2 M Tris–HCl buffer (pH 9, strength = 0.2 M, 30 °C) at 0.21 g L−1 H−1 and 98.6 ± 1.3 wt%, respectively. There is a congruity vis‐à‐vis to specific buffer type, molarity, pH, ionic strength, and temperature values for superior in vivo depolymerization activities. Direct products from in vivo depolymerization matched the individual monomeric composition of native mcl‐PHA. It points to exo‐type reaction for the in vivo process, and potential biological route to chiral molecules. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Biotechnology & Applied Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1002/bab.1666
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        Text: English
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      – SubjectFull: Biosynthesis
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      – SubjectFull: Depolymerization
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      – SubjectFull: Pseudomonas putida
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      – TitleFull: Microbial biosynthesis and in vivo depolymerization of intracellular medium‐chain‐length poly‐3‐hydroxyalkanoates as potential route to platform chemicals.
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            – D: 01
              M: 11
              Text: Nov/Dec2018
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              Y: 2018
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