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Polyelectrolyte-coated ultra-small nanoparticles with Tb(III)-centered luminescence as cell labels with unusual charge effect on their cell internalization.

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Title: Polyelectrolyte-coated ultra-small nanoparticles with Tb(III)-centered luminescence as cell labels with unusual charge effect on their cell internalization.
Authors: Zairov, Rustem R.1 rustem@iopc.ru, Solovieva, Anastasiya O.1, Shamsutdinova, Nataliya A.1, Podyachev, Sergey N.1, Shestopalov, Michael A.1, Pozmogova, Tatiana N.1, Miroshnichenko, Svetlana M.1, Mustafina, Asiya R.1, Karasik, Andrey A.1
Source: Materials Science & Engineering: C. Feb2019, Vol. 95, p166-173. 8p.
Subjects: Polyelectrolytes, Luminescence, Nanoparticles, Terbium, Metal complexes, Energy transfer
Abstract: Abstract The present work reports ultra-small polyelectrolyte-coated water insoluble Tb(III) complex species with bright Tb(III)-centered luminescence resulted from efficient ligand-to-metal energy transfer as efficient labels for Hep-2 cells. The flow cytometry data revealed the enhanced cellular uptake of negatively charged nanoparticles coated by the polystyrenesulfonate (PSS)-monolayer versus the positively charged nanoparticles. The latter are obtained by layer-by-layer deposition of polyethyleneimine (PEI) onto PSS-coated ones. Confocal and TEM images of Hep-2 cells exposed by the colloids confirm favorable cell internalization of the PSS- compared to PEI-PSS-coated colloids illustrating unusual charge-effect. Dynamic light scattering data indicate significant effect of the biological background exemplified by serum bovine albumin and phosphatidylcholine-based bilayers on the exterior charge and aggregation behavior of the colloids. The obtained results reveal the PSS-coated nanoparticles based on water insoluble Tb(III) complex as promising cell labels. Highlights • Polyelectrolyte-coated nanoparticles with green Tb-luminescence for cell imaging • Layer-by-layer technique for variation of the exterior polyelectrolyte coating • Recharging and de-aggregation of the nanoparticles in biological background • High viability of Hep-2 cells incubated by the nanoparticles • Impact of the exterior polyelectrolyte layer on the cell internalization [ABSTRACT FROM AUTHOR]
Copyright of Materials Science & Engineering: C is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
An: 133599512
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  Data: Polyelectrolyte-coated ultra-small nanoparticles with Tb(III)-centered luminescence as cell labels with unusual charge effect on their cell internalization.
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  Data: <searchLink fieldCode="JN" term="%22Materials+Science+%26+Engineering%3A+C%22">Materials Science & Engineering: C</searchLink>. Feb2019, Vol. 95, p166-173. 8p.
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  Data: <searchLink fieldCode="DE" term="%22Polyelectrolytes%22">Polyelectrolytes</searchLink><br /><searchLink fieldCode="DE" term="%22Luminescence%22">Luminescence</searchLink><br /><searchLink fieldCode="DE" term="%22Nanoparticles%22">Nanoparticles</searchLink><br /><searchLink fieldCode="DE" term="%22Terbium%22">Terbium</searchLink><br /><searchLink fieldCode="DE" term="%22Metal+complexes%22">Metal complexes</searchLink><br /><searchLink fieldCode="DE" term="%22Energy+transfer%22">Energy transfer</searchLink>
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  Data: Abstract The present work reports ultra-small polyelectrolyte-coated water insoluble Tb(III) complex species with bright Tb(III)-centered luminescence resulted from efficient ligand-to-metal energy transfer as efficient labels for Hep-2 cells. The flow cytometry data revealed the enhanced cellular uptake of negatively charged nanoparticles coated by the polystyrenesulfonate (PSS)-monolayer versus the positively charged nanoparticles. The latter are obtained by layer-by-layer deposition of polyethyleneimine (PEI) onto PSS-coated ones. Confocal and TEM images of Hep-2 cells exposed by the colloids confirm favorable cell internalization of the PSS- compared to PEI-PSS-coated colloids illustrating unusual charge-effect. Dynamic light scattering data indicate significant effect of the biological background exemplified by serum bovine albumin and phosphatidylcholine-based bilayers on the exterior charge and aggregation behavior of the colloids. The obtained results reveal the PSS-coated nanoparticles based on water insoluble Tb(III) complex as promising cell labels. Highlights • Polyelectrolyte-coated nanoparticles with green Tb-luminescence for cell imaging • Layer-by-layer technique for variation of the exterior polyelectrolyte coating • Recharging and de-aggregation of the nanoparticles in biological background • High viability of Hep-2 cells incubated by the nanoparticles • Impact of the exterior polyelectrolyte layer on the cell internalization [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Materials Science & Engineering: C is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.msec.2018.10.084
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      – SubjectFull: Luminescence
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              Text: Feb2019
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