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Simple interface for scanning chemical compounds on developed thin layer chromatography plates using electrospray ionization mass spectrometry.

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Title: Simple interface for scanning chemical compounds on developed thin layer chromatography plates using electrospray ionization mass spectrometry.
Authors: Cheng, Sy-Chyi1, Bhat, Suhail Muzaffar1, Lee, Chi-Wei2,3, Shiea, Jentaie1,4,5,6 jetea@mail.nsysu.edu.tw
Source: Analytica Chimica Acta. Feb2019, Vol. 1049, p1-9. 9p.
Subjects: Thin layer chromatography, Electrospray ionization mass spectrometry, Methanol, High voltages, Alkaloids, Scopolamine
Abstract: Abstract A simple and cheap design for interfacing thin layer chromatography (TLC) with electrospray ionization mass spectrometry (ESI/MS) was developed to scan and characterize compounds on TLC plate. The developed TLC plate was rapidly and easily modified into two sawtooth-edged pieces that were positioned on an XYZ stage so that one of the triangular tips was pointed toward the MS inlet. A drop of methanol and high DC voltage was applied at the tip to induce ESI. After the analytes in the first tip were analyzed, the TLC piece was moved so that the second triangular tip was pointed toward the MS inlet for analysis. The process was repeated until all the triangular tips on the piece were analyzed. In this manner, the analytes, no matter visible or non-visible bands, were scanned and characterized. Since a 4.8 cm long TLC track were cut to 32 triangles on two sawtooth pieces for analysis, the spatial resolution of using the sawtooth TLC-ESI/MS for analysis is 1.5 mm/band. A mixture of dye standards and Datura metel flower extract was analyzed to demonstrate the capability of sawtooth TLC-ESI/MS on scanning and characterizing chemical compounds on the TLC plates. The limits of detection of the dye standards were between 0.25 and 2.5 ng/band. TLC bands containing alkaloids such as scopolamine and norscopolamine from the Datura metel flower extract were not visualized on the developed TLC track, but were successfully detected at different triangular tips using sawtooth TLC-ESI/MS. Based on these results, the R f values of scopolamine and norscopolamine were determined. Graphical abstract Image 1 Highlights • Sawtooth TLC-ESI/MS was developed to characterize a mixture. • The developed TLC plate was modified into two sawtooth-edged pieces for inducing ESI at the triangular tips. • The entire TLC track was scanned with minimal gel particle detachment during analysis. • Visualizable and non-visualizable bands were both characterized, so that their R f information was elucidated. [ABSTRACT FROM AUTHOR]
Copyright of Analytica Chimica Acta is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
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Items – Name: Title
  Label: Title
  Group: Ti
  Data: Simple interface for scanning chemical compounds on developed thin layer chromatography plates using electrospray ionization mass spectrometry.
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  Data: <searchLink fieldCode="AR" term="%22Cheng%2C+Sy-Chyi%22">Cheng, Sy-Chyi</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Bhat%2C+Suhail+Muzaffar%22">Bhat, Suhail Muzaffar</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Lee%2C+Chi-Wei%22">Lee, Chi-Wei</searchLink><relatesTo>2,3</relatesTo><br /><searchLink fieldCode="AR" term="%22Shiea%2C+Jentaie%22">Shiea, Jentaie</searchLink><relatesTo>1,4,5,6</relatesTo><i> jetea@mail.nsysu.edu.tw</i>
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  Data: <searchLink fieldCode="JN" term="%22Analytica+Chimica+Acta%22">Analytica Chimica Acta</searchLink>. Feb2019, Vol. 1049, p1-9. 9p.
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  Data: <searchLink fieldCode="DE" term="%22Thin+layer+chromatography%22">Thin layer chromatography</searchLink><br /><searchLink fieldCode="DE" term="%22Electrospray+ionization+mass+spectrometry%22">Electrospray ionization mass spectrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Methanol%22">Methanol</searchLink><br /><searchLink fieldCode="DE" term="%22High+voltages%22">High voltages</searchLink><br /><searchLink fieldCode="DE" term="%22Alkaloids%22">Alkaloids</searchLink><br /><searchLink fieldCode="DE" term="%22Scopolamine%22">Scopolamine</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Abstract A simple and cheap design for interfacing thin layer chromatography (TLC) with electrospray ionization mass spectrometry (ESI/MS) was developed to scan and characterize compounds on TLC plate. The developed TLC plate was rapidly and easily modified into two sawtooth-edged pieces that were positioned on an XYZ stage so that one of the triangular tips was pointed toward the MS inlet. A drop of methanol and high DC voltage was applied at the tip to induce ESI. After the analytes in the first tip were analyzed, the TLC piece was moved so that the second triangular tip was pointed toward the MS inlet for analysis. The process was repeated until all the triangular tips on the piece were analyzed. In this manner, the analytes, no matter visible or non-visible bands, were scanned and characterized. Since a 4.8 cm long TLC track were cut to 32 triangles on two sawtooth pieces for analysis, the spatial resolution of using the sawtooth TLC-ESI/MS for analysis is 1.5 mm/band. A mixture of dye standards and Datura metel flower extract was analyzed to demonstrate the capability of sawtooth TLC-ESI/MS on scanning and characterizing chemical compounds on the TLC plates. The limits of detection of the dye standards were between 0.25 and 2.5 ng/band. TLC bands containing alkaloids such as scopolamine and norscopolamine from the Datura metel flower extract were not visualized on the developed TLC track, but were successfully detected at different triangular tips using sawtooth TLC-ESI/MS. Based on these results, the R f values of scopolamine and norscopolamine were determined. Graphical abstract Image 1 Highlights • Sawtooth TLC-ESI/MS was developed to characterize a mixture. • The developed TLC plate was modified into two sawtooth-edged pieces for inducing ESI at the triangular tips. • The entire TLC track was scanned with minimal gel particle detachment during analysis. • Visualizable and non-visualizable bands were both characterized, so that their R f information was elucidated. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Analytica Chimica Acta is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.aca.2018.10.042
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      – Code: eng
        Text: English
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      Pagination:
        PageCount: 9
        StartPage: 1
    Subjects:
      – SubjectFull: Thin layer chromatography
        Type: general
      – SubjectFull: Electrospray ionization mass spectrometry
        Type: general
      – SubjectFull: Methanol
        Type: general
      – SubjectFull: High voltages
        Type: general
      – SubjectFull: Alkaloids
        Type: general
      – SubjectFull: Scopolamine
        Type: general
    Titles:
      – TitleFull: Simple interface for scanning chemical compounds on developed thin layer chromatography plates using electrospray ionization mass spectrometry.
        Type: main
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            NameFull: Cheng, Sy-Chyi
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            NameFull: Bhat, Suhail Muzaffar
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            NameFull: Lee, Chi-Wei
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            NameFull: Shiea, Jentaie
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            – D: 21
              M: 02
              Text: Feb2019
              Type: published
              Y: 2019
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              Value: 1049
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            – TitleFull: Analytica Chimica Acta
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