Identification of target muscle-proteins using Western blotting and high-resolution mass spectrometry as early quality indicators of nutrient supply practices in rainbow trout (Oncorhynchus mykiss).
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| Title: | Identification of target muscle-proteins using Western blotting and high-resolution mass spectrometry as early quality indicators of nutrient supply practices in rainbow trout (Oncorhynchus mykiss). |
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| Authors: | Bongiorno, Tiziana1 tiziana.bongiorno@uniud.it, Cancian, Giulia1 giulia.cancian001@spes.uniud.it, Buhler, Sofie2 sofie.buhler@yahoo.it, Tibaldi, Emilio1 emilio.tibaldi@uniud.it, Sforza, Stefano2 stefano.sforza@unipr.it, Lippe, Giovanna1 giovanna.lippe@uniud.it, Stecchini, Mara Lucia1 mara.stecchini@uniud.it |
| Source: | European Food Research & Technology. Feb2019, Vol. 245 Issue 2, p401-410. 10p. |
| Subjects: | Rainbow trout, Muscle proteins, Mass spectrometry, Reticulon proteins, Fish fillets |
| Abstract: | Using Western blotting and mass spectrometry we investigated the changes in trout muscle proteins as affected by pre-slaughter nutrient supply practices, including starvation and feed restriction, in comparison to full feeding. A number of fillet quality attributes were also analyzed. During cold storage, fillet firmness was not influenced by the feeding practices, while the initial pH was higher in the treated trout. The early appearance of an alpha-actin 26 kDa-fragment and the detection of enigma protein were both signs of impaired muscle integrity, observed only in the restricted conditions, where water holding capacity was initially lower. The detection of reticulon involved in authophagy and the early finding of apolipoprotein A-I-1 fragments in these restricted samples was consistent with their early degradation in comparison to full ration controls. Since specific protein changes reflected differences in feed management, the use of selected muscle proteins as sensitive indicators of fillet quality is proposed. [ABSTRACT FROM AUTHOR] |
| Copyright of European Food Research & Technology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 134079054 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Identification of target muscle-proteins using Western blotting and high-resolution mass spectrometry as early quality indicators of nutrient supply practices in rainbow trout (Oncorhynchus mykiss). – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Bongiorno%2C+Tiziana%22">Bongiorno, Tiziana</searchLink><relatesTo>1</relatesTo><i> tiziana.bongiorno@uniud.it</i><br /><searchLink fieldCode="AR" term="%22Cancian%2C+Giulia%22">Cancian, Giulia</searchLink><relatesTo>1</relatesTo><i> giulia.cancian001@spes.uniud.it</i><br /><searchLink fieldCode="AR" term="%22Buhler%2C+Sofie%22">Buhler, Sofie</searchLink><relatesTo>2</relatesTo><i> sofie.buhler@yahoo.it</i><br /><searchLink fieldCode="AR" term="%22Tibaldi%2C+Emilio%22">Tibaldi, Emilio</searchLink><relatesTo>1</relatesTo><i> emilio.tibaldi@uniud.it</i><br /><searchLink fieldCode="AR" term="%22Sforza%2C+Stefano%22">Sforza, Stefano</searchLink><relatesTo>2</relatesTo><i> stefano.sforza@unipr.it</i><br /><searchLink fieldCode="AR" term="%22Lippe%2C+Giovanna%22">Lippe, Giovanna</searchLink><relatesTo>1</relatesTo><i> giovanna.lippe@uniud.it</i><br /><searchLink fieldCode="AR" term="%22Stecchini%2C+Mara+Lucia%22">Stecchini, Mara Lucia</searchLink><relatesTo>1</relatesTo><i> mara.stecchini@uniud.it</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22European+Food+Research+%26+Technology%22">European Food Research & Technology</searchLink>. Feb2019, Vol. 245 Issue 2, p401-410. 10p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Rainbow+trout%22">Rainbow trout</searchLink><br /><searchLink fieldCode="DE" term="%22Muscle+proteins%22">Muscle proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Mass+spectrometry%22">Mass spectrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Reticulon+proteins%22">Reticulon proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Fish+fillets%22">Fish fillets</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Using Western blotting and mass spectrometry we investigated the changes in trout muscle proteins as affected by pre-slaughter nutrient supply practices, including starvation and feed restriction, in comparison to full feeding. A number of fillet quality attributes were also analyzed. During cold storage, fillet firmness was not influenced by the feeding practices, while the initial pH was higher in the treated trout. The early appearance of an alpha-actin 26 kDa-fragment and the detection of enigma protein were both signs of impaired muscle integrity, observed only in the restricted conditions, where water holding capacity was initially lower. The detection of reticulon involved in authophagy and the early finding of apolipoprotein A-I-1 fragments in these restricted samples was consistent with their early degradation in comparison to full ration controls. Since specific protein changes reflected differences in feed management, the use of selected muscle proteins as sensitive indicators of fillet quality is proposed. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of European Food Research & Technology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00217-018-3172-7 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 10 StartPage: 401 Subjects: – SubjectFull: Rainbow trout Type: general – SubjectFull: Muscle proteins Type: general – SubjectFull: Mass spectrometry Type: general – SubjectFull: Reticulon proteins Type: general – SubjectFull: Fish fillets Type: general Titles: – TitleFull: Identification of target muscle-proteins using Western blotting and high-resolution mass spectrometry as early quality indicators of nutrient supply practices in rainbow trout (Oncorhynchus mykiss). Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Bongiorno, Tiziana – PersonEntity: Name: NameFull: Cancian, Giulia – PersonEntity: Name: NameFull: Buhler, Sofie – PersonEntity: Name: NameFull: Tibaldi, Emilio – PersonEntity: Name: NameFull: Sforza, Stefano – PersonEntity: Name: NameFull: Lippe, Giovanna – PersonEntity: Name: NameFull: Stecchini, Mara Lucia IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 02 Text: Feb2019 Type: published Y: 2019 Identifiers: – Type: issn-print Value: 14382377 Numbering: – Type: volume Value: 245 – Type: issue Value: 2 Titles: – TitleFull: European Food Research & Technology Type: main |
| ResultId | 1 |
