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Synthesis of polyhydroxyalkanoates through the biodegradation of poly(cis-1,4-isoprene) rubber.

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Title: Synthesis of polyhydroxyalkanoates through the biodegradation of poly(cis-1,4-isoprene) rubber.
Authors: Andler, Rodrigo1 randler@ucm.cl, Vivod, Robin2, Steinbüchel, Alexander2
Source: Journal of Bioscience & Bioengineering. Mar2019, Vol. 127 Issue 3, p360-365. 6p.
Subjects: Polyhydroxyalkanoates, Waste products, Biodegradable plastics, Monomers, Plasmids
Abstract: The search of alternative substrates for the synthesis of polyhydroxyalkanoates (PHA) has become an important factor in order to decrease the production costs. Therefore, the use of industrial by-products or waste materials as carbon and energy sources for different PHA-producing microorganisms has been evaluated during the last decades. Recombinant strains of Gordonia polyisoprenivorans VH2 harboring plasmid pAK68, which contains phaCAB from Ralstonia eutropha and plasmid pAK71 comprising phaC1 from Pseudomonas aeruginosa were evaluated for PHA production. Cultivations were performed in shake flasks, using different carbon sources under an N-starvation condition. Having in consideration the rubber degrading capability of the actinomycete, poly(cis -1,4-isoprene) was utilized as sole carbon source. After twenty days of cultivation the PHA content was analyzed using GC-MS. In cultures of G. polyisoprenivorans harboring pAK68, the detection of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) monomer units indicated the accumulation of the copolyester poly(3HB- co -3HV). This study proposes a recycling method for rubber waste through its biotransformation into bioplastic. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Bioscience & Bioengineering is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 134532427
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  Label: Title
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  Data: Synthesis of polyhydroxyalkanoates through the biodegradation of poly(cis-1,4-isoprene) rubber.
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  Data: <searchLink fieldCode="AR" term="%22Andler%2C+Rodrigo%22">Andler, Rodrigo</searchLink><relatesTo>1</relatesTo><i> randler@ucm.cl</i><br /><searchLink fieldCode="AR" term="%22Vivod%2C+Robin%22">Vivod, Robin</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Steinbüchel%2C+Alexander%22">Steinbüchel, Alexander</searchLink><relatesTo>2</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Bioscience+%26+Bioengineering%22">Journal of Bioscience & Bioengineering</searchLink>. Mar2019, Vol. 127 Issue 3, p360-365. 6p.
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  Data: <searchLink fieldCode="DE" term="%22Polyhydroxyalkanoates%22">Polyhydroxyalkanoates</searchLink><br /><searchLink fieldCode="DE" term="%22Waste+products%22">Waste products</searchLink><br /><searchLink fieldCode="DE" term="%22Biodegradable+plastics%22">Biodegradable plastics</searchLink><br /><searchLink fieldCode="DE" term="%22Monomers%22">Monomers</searchLink><br /><searchLink fieldCode="DE" term="%22Plasmids%22">Plasmids</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The search of alternative substrates for the synthesis of polyhydroxyalkanoates (PHA) has become an important factor in order to decrease the production costs. Therefore, the use of industrial by-products or waste materials as carbon and energy sources for different PHA-producing microorganisms has been evaluated during the last decades. Recombinant strains of Gordonia polyisoprenivorans VH2 harboring plasmid pAK68, which contains phaCAB from Ralstonia eutropha and plasmid pAK71 comprising phaC1 from Pseudomonas aeruginosa were evaluated for PHA production. Cultivations were performed in shake flasks, using different carbon sources under an N-starvation condition. Having in consideration the rubber degrading capability of the actinomycete, poly(cis -1,4-isoprene) was utilized as sole carbon source. After twenty days of cultivation the PHA content was analyzed using GC-MS. In cultures of G. polyisoprenivorans harboring pAK68, the detection of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) monomer units indicated the accumulation of the copolyester poly(3HB- co -3HV). This study proposes a recycling method for rubber waste through its biotransformation into bioplastic. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Bioscience & Bioengineering is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.jbiosc.2018.08.015
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      – Code: eng
        Text: English
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        PageCount: 6
        StartPage: 360
    Subjects:
      – SubjectFull: Polyhydroxyalkanoates
        Type: general
      – SubjectFull: Waste products
        Type: general
      – SubjectFull: Biodegradable plastics
        Type: general
      – SubjectFull: Monomers
        Type: general
      – SubjectFull: Plasmids
        Type: general
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      – TitleFull: Synthesis of polyhydroxyalkanoates through the biodegradation of poly(cis-1,4-isoprene) rubber.
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            NameFull: Andler, Rodrigo
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            NameFull: Vivod, Robin
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            NameFull: Steinbüchel, Alexander
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            – D: 01
              M: 03
              Text: Mar2019
              Type: published
              Y: 2019
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              Value: 127
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            – TitleFull: Journal of Bioscience & Bioengineering
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