Hybrid capture data unravel a rapid radiation of pimpliform parasitoid wasps (Hymenoptera: Ichneumonidae: Pimpliformes).
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| Title: | Hybrid capture data unravel a rapid radiation of pimpliform parasitoid wasps (Hymenoptera: Ichneumonidae: Pimpliformes). |
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| Authors: | Klopfstein, Seraina1,2 klopfstein@nmbe.ch, Langille, Barbara3, Spasojevic, Tamara1,2, Broad, Gavin R.4, Cooper, Steven J. B.3,5, Austin, Andrew D.3, Niehuis, Oliver6 |
| Source: | Systematic Entomology. Apr2019, Vol. 44 Issue 2, p361-383. 23p. |
| Subjects: | Wasp behavior, Insect phylogeny, Molecular phylogeny, Nucleotide sequence, Polymerase chain reaction |
| Abstract: | The parasitoid wasp family Ichneumonidae is among the most diverse groups of organisms, with conservative estimates suggesting that it contains more species than all vertebrates together. However, ichneumonids are also among the most severely understudied groups, and our understanding of their evolution is hampered by the lack of a robust higher‐level phylogeny of this group. Based on newly generated transcriptome sequence data, which were filtered according to several criteria of phylogenetic informativeness, we developed target DNA enrichment baits to capture 93 genes across species of Ichneumonidae. The baits were applied to DNA of 55 ichneumonids, with a focus on Pimpliformes, an informal group containing nine subfamilies. Phylogenetic trees were inferred under maximum likelihood and Bayesian approaches, at both the nucleotide and amino acid levels. We found maximum support for the monophyly of Pimpliformes but low resolution and very short branches close to its base, strongly suggesting a rapid radiation. Two genera and one genus‐group were consistently recovered in unexpected parts of the tree, prompting changes in their higher‐level classification: Pseudorhyssa Merrill, currently classified in the subfamily Poemeniinae, is transferred to the tribe Delomeristini within Pimplinae, and Hemiphanes Förster is moved from Orthocentrinae to Cryptinae. Likewise, the tribe Theroniini is resurrected for the Theronia group of genera (stat. rev.). Phylogenetic analyses, in which we gradually increased the numbers of genes, revealed that the initially steep increase in mean clade support slows down at around 40 genes, and consideration of up to 93 genes still left various nodes in the inferred phylogenetic tree poorly resolved. It remains to be shown whether more extensive gene or taxon sampling can resolve the early evolution of the pimpliform subfamilies. Based on newly generated transcriptome data for several ichneumonid taxa, we developed DNA enrichment baits to capture 93 genes across species of pimpliform parasitoid wasps.Assessments of phylogenetic informativeness of the different genes and in‐depth phylogenetic analyses demonstrate that Pimpliformes underwent a very rapid radiation – perhaps the most rapid one demonstrated to date in parasitoids.Our results have implications for subfamily classification, especially for the genera Hemiphanes and Pseudorhyssa, and for the Theronia genus‐group. [ABSTRACT FROM AUTHOR] |
| Copyright of Systematic Entomology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Header | DbId: egs DbLabel: Engineering Source An: 135035705 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Hybrid capture data unravel a rapid radiation of pimpliform parasitoid wasps (Hymenoptera: Ichneumonidae: Pimpliformes). – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Klopfstein%2C+Seraina%22">Klopfstein, Seraina</searchLink><relatesTo>1,2</relatesTo><i> klopfstein@nmbe.ch</i><br /><searchLink fieldCode="AR" term="%22Langille%2C+Barbara%22">Langille, Barbara</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Spasojevic%2C+Tamara%22">Spasojevic, Tamara</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Broad%2C+Gavin+R%2E%22">Broad, Gavin R.</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Cooper%2C+Steven+J%2E+B%2E%22">Cooper, Steven J. B.</searchLink><relatesTo>3,5</relatesTo><br /><searchLink fieldCode="AR" term="%22Austin%2C+Andrew+D%2E%22">Austin, Andrew D.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Niehuis%2C+Oliver%22">Niehuis, Oliver</searchLink><relatesTo>6</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Systematic+Entomology%22">Systematic Entomology</searchLink>. Apr2019, Vol. 44 Issue 2, p361-383. 23p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Wasp+behavior%22">Wasp behavior</searchLink><br /><searchLink fieldCode="DE" term="%22Insect+phylogeny%22">Insect phylogeny</searchLink><br /><searchLink fieldCode="DE" term="%22Molecular+phylogeny%22">Molecular phylogeny</searchLink><br /><searchLink fieldCode="DE" term="%22Nucleotide+sequence%22">Nucleotide sequence</searchLink><br /><searchLink fieldCode="DE" term="%22Polymerase+chain+reaction%22">Polymerase chain reaction</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The parasitoid wasp family Ichneumonidae is among the most diverse groups of organisms, with conservative estimates suggesting that it contains more species than all vertebrates together. However, ichneumonids are also among the most severely understudied groups, and our understanding of their evolution is hampered by the lack of a robust higher‐level phylogeny of this group. Based on newly generated transcriptome sequence data, which were filtered according to several criteria of phylogenetic informativeness, we developed target DNA enrichment baits to capture 93 genes across species of Ichneumonidae. The baits were applied to DNA of 55 ichneumonids, with a focus on Pimpliformes, an informal group containing nine subfamilies. Phylogenetic trees were inferred under maximum likelihood and Bayesian approaches, at both the nucleotide and amino acid levels. We found maximum support for the monophyly of Pimpliformes but low resolution and very short branches close to its base, strongly suggesting a rapid radiation. Two genera and one genus‐group were consistently recovered in unexpected parts of the tree, prompting changes in their higher‐level classification: Pseudorhyssa Merrill, currently classified in the subfamily Poemeniinae, is transferred to the tribe Delomeristini within Pimplinae, and Hemiphanes Förster is moved from Orthocentrinae to Cryptinae. Likewise, the tribe Theroniini is resurrected for the Theronia group of genera (stat. rev.). Phylogenetic analyses, in which we gradually increased the numbers of genes, revealed that the initially steep increase in mean clade support slows down at around 40 genes, and consideration of up to 93 genes still left various nodes in the inferred phylogenetic tree poorly resolved. It remains to be shown whether more extensive gene or taxon sampling can resolve the early evolution of the pimpliform subfamilies. Based on newly generated transcriptome data for several ichneumonid taxa, we developed DNA enrichment baits to capture 93 genes across species of pimpliform parasitoid wasps.Assessments of phylogenetic informativeness of the different genes and in‐depth phylogenetic analyses demonstrate that Pimpliformes underwent a very rapid radiation – perhaps the most rapid one demonstrated to date in parasitoids.Our results have implications for subfamily classification, especially for the genera Hemiphanes and Pseudorhyssa, and for the Theronia genus‐group. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Systematic Entomology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1111/syen.12333 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 23 StartPage: 361 Subjects: – SubjectFull: Wasp behavior Type: general – SubjectFull: Insect phylogeny Type: general – SubjectFull: Molecular phylogeny Type: general – SubjectFull: Nucleotide sequence Type: general – SubjectFull: Polymerase chain reaction Type: general Titles: – TitleFull: Hybrid capture data unravel a rapid radiation of pimpliform parasitoid wasps (Hymenoptera: Ichneumonidae: Pimpliformes). Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Klopfstein, Seraina – PersonEntity: Name: NameFull: Langille, Barbara – PersonEntity: Name: NameFull: Spasojevic, Tamara – PersonEntity: Name: NameFull: Broad, Gavin R. – PersonEntity: Name: NameFull: Cooper, Steven J. B. – PersonEntity: Name: NameFull: Austin, Andrew D. – PersonEntity: Name: NameFull: Niehuis, Oliver IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 04 Text: Apr2019 Type: published Y: 2019 Identifiers: – Type: issn-print Value: 03076970 Numbering: – Type: volume Value: 44 – Type: issue Value: 2 Titles: – TitleFull: Systematic Entomology Type: main |
| ResultId | 1 |
