Development of Biosensor for 3-Hydroxypropionic Acid.
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| Title: | Development of Biosensor for 3-Hydroxypropionic Acid. |
|---|---|
| Authors: | Nguyen, Nam Hoai1,2, Kim, Jung-Rae1, Park, Sunghoon1,2 parksh@unist.ac.kr |
| Source: | Biotechnology & Bioprocess Engineering. Feb2019, Vol. 24 Issue 1, p109-118. 10p. |
| Subjects: | Biosensors, Transcription factors |
| Abstract: | In this study, whole-cell biosensors that detect and indicate the concentration of 3-hydroxypropionic acid (3-HP) by producing a green fluorescent signal were developed using a transcription factor (TF)-responsive 3-HP inducible promoter identified in the Pseudomonas denitrificans 3-HP degradation pathway. Upon forming a complex with 3-HP, the MmsR TF protein, a LysR-type transcriptional regulator (LTTR), binds to the PmmsA promoter and controls the expression of the PmmsA-regulated gfp reporter gene in response to the concentration of 3-HP. Furthermore, by enhancing the expression of MmsR and through mutagenesis of the PmmsA promoter region, three well-performing biosensors were developed that covered a wide dynamic range of 3-HP (0.01-100 mM when added externally) with ~100-fold signal change upon induction in P. denitrificans. The 3-HP biosensor machinery, composed of MmsR, the PmmsA promoter and gfp could also function well in E. coli and P. putida. The developed 3-HP biosensors should be useful for engineering 3-HP-producing strains and the enzymes associated with its production. [ABSTRACT FROM AUTHOR] |
| Copyright of Biotechnology & Bioprocess Engineering is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 135394683 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Development of Biosensor for 3-Hydroxypropionic Acid. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Nguyen%2C+Nam+Hoai%22">Nguyen, Nam Hoai</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Kim%2C+Jung-Rae%22">Kim, Jung-Rae</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Park%2C+Sunghoon%22">Park, Sunghoon</searchLink><relatesTo>1,2</relatesTo><i> parksh@unist.ac.kr</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Biotechnology+%26+Bioprocess+Engineering%22">Biotechnology & Bioprocess Engineering</searchLink>. Feb2019, Vol. 24 Issue 1, p109-118. 10p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Biosensors%22">Biosensors</searchLink><br /><searchLink fieldCode="DE" term="%22Transcription+factors%22">Transcription factors</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: In this study, whole-cell biosensors that detect and indicate the concentration of 3-hydroxypropionic acid (3-HP) by producing a green fluorescent signal were developed using a transcription factor (TF)-responsive 3-HP inducible promoter identified in the Pseudomonas denitrificans 3-HP degradation pathway. Upon forming a complex with 3-HP, the MmsR TF protein, a LysR-type transcriptional regulator (LTTR), binds to the PmmsA promoter and controls the expression of the PmmsA-regulated gfp reporter gene in response to the concentration of 3-HP. Furthermore, by enhancing the expression of MmsR and through mutagenesis of the PmmsA promoter region, three well-performing biosensors were developed that covered a wide dynamic range of 3-HP (0.01-100 mM when added externally) with ~100-fold signal change upon induction in P. denitrificans. The 3-HP biosensor machinery, composed of MmsR, the PmmsA promoter and gfp could also function well in E. coli and P. putida. The developed 3-HP biosensors should be useful for engineering 3-HP-producing strains and the enzymes associated with its production. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Biotechnology & Bioprocess Engineering is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s12257-018-0380-8 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 10 StartPage: 109 Subjects: – SubjectFull: Biosensors Type: general – SubjectFull: Transcription factors Type: general Titles: – TitleFull: Development of Biosensor for 3-Hydroxypropionic Acid. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Nguyen, Nam Hoai – PersonEntity: Name: NameFull: Kim, Jung-Rae – PersonEntity: Name: NameFull: Park, Sunghoon IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 02 Text: Feb2019 Type: published Y: 2019 Identifiers: – Type: issn-print Value: 12268372 Numbering: – Type: volume Value: 24 – Type: issue Value: 1 Titles: – TitleFull: Biotechnology & Bioprocess Engineering Type: main |
| ResultId | 1 |
