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Crimean-Congo hemorrhagic fever virus nucleocapsid protein harbors distinct RNA-binding sites in the stalk and head domains.

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Title: Crimean-Congo hemorrhagic fever virus nucleocapsid protein harbors distinct RNA-binding sites in the stalk and head domains.
Authors: Jeeva, Subbiah1, Mir, Sheema2, Velasquez, Adrain3, Ragan, Jacquelyn1, Leka, Aljona3, Sharon Wu3, Sevarany, Ariga Tahmasian3, Royster, Austin D.3, Almeida, Nicholas A.3, Fion Chan3, O'Brien, Lea3, Mir, Mohammad Ayoub1 mmir@westernu.edu
Source: Journal of Biological Chemistry. 3/29/2019, Vol. 294 Issue 13, p5023-5037. 15p.
Subjects: Hemorrhagic fever, Viral proteins, Virus diseases, Protein domains, Stalking, Base pairs
Abstract: Crimean-Congo hemorrhagic fever virus (CCHFV) is a tickborne Nairovirus that causes severe hemorrhagic fever with a mortality rate of up to 30% in certain outbreaks worldwide. The virus has wide endemic distribution. There is no effective antiviral therapeutic orFDAapproved vaccine for this zoonotic viral illness. The multifunctional CCHFV nucleocapsid protein (N protein) plays a crucial role in the establishment of viral infection and is an important structural component of the virion. Here we show thatCCHFVNprotein has a distant RNA-binding site in the stalk domain that specifically recognizes the vRNA panhandle, formed by the base pairing of complementary nucleotides at the 5' and 3' termini of the vRNA genome. Using multiple approaches, including filter-bonding analysis, GFP reporter assay, and biolayer interferometry we observed an N protein-panhandle interaction both in vitro and in vivo. The purified WT CCHFV N protein and the stalk domain also recognize the vRNA panhandle of hazara virus, another Nairovirus in the family Bunyaviridae, demonstrating the genus-specific nature ofNprotein-panhandle interaction. Another RNA-binding site was identified at the head domain of CCHFV N protein that nonspecifically recognizes the single strand RNA (ssRNA) of viral or nonviral origin. Expression of CCHFVNprotein stalk domain active in panhandle binding, dramatically inhibited the hazara virus replication in cell culture, illustrating the role of N protein-panhandle interaction in Nairovirus replication. Our findings reveal the stalk domain of N protein as a potential target in therapeutic interventions to manage CCHFV disease. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Biological Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
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  Data: Crimean-Congo hemorrhagic fever virus nucleocapsid protein harbors distinct RNA-binding sites in the stalk and head domains.
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  Data: <searchLink fieldCode="AR" term="%22Jeeva%2C+Subbiah%22">Jeeva, Subbiah</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Mir%2C+Sheema%22">Mir, Sheema</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Velasquez%2C+Adrain%22">Velasquez, Adrain</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Ragan%2C+Jacquelyn%22">Ragan, Jacquelyn</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Leka%2C+Aljona%22">Leka, Aljona</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Sharon+Wu%22">Sharon Wu</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Sevarany%2C+Ariga+Tahmasian%22">Sevarany, Ariga Tahmasian</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Royster%2C+Austin+D%2E%22">Royster, Austin D.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Almeida%2C+Nicholas+A%2E%22">Almeida, Nicholas A.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Fion+Chan%22">Fion Chan</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22O'Brien%2C+Lea%22">O'Brien, Lea</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Mir%2C+Mohammad+Ayoub%22">Mir, Mohammad Ayoub</searchLink><relatesTo>1</relatesTo><i> mmir@westernu.edu</i>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Biological+Chemistry%22">Journal of Biological Chemistry</searchLink>. 3/29/2019, Vol. 294 Issue 13, p5023-5037. 15p.
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  Data: <searchLink fieldCode="DE" term="%22Hemorrhagic+fever%22">Hemorrhagic fever</searchLink><br /><searchLink fieldCode="DE" term="%22Viral+proteins%22">Viral proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Virus+diseases%22">Virus diseases</searchLink><br /><searchLink fieldCode="DE" term="%22Protein+domains%22">Protein domains</searchLink><br /><searchLink fieldCode="DE" term="%22Stalking%22">Stalking</searchLink><br /><searchLink fieldCode="DE" term="%22Base+pairs%22">Base pairs</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: Crimean-Congo hemorrhagic fever virus (CCHFV) is a tickborne Nairovirus that causes severe hemorrhagic fever with a mortality rate of up to 30% in certain outbreaks worldwide. The virus has wide endemic distribution. There is no effective antiviral therapeutic orFDAapproved vaccine for this zoonotic viral illness. The multifunctional CCHFV nucleocapsid protein (N protein) plays a crucial role in the establishment of viral infection and is an important structural component of the virion. Here we show thatCCHFVNprotein has a distant RNA-binding site in the stalk domain that specifically recognizes the vRNA panhandle, formed by the base pairing of complementary nucleotides at the 5' and 3' termini of the vRNA genome. Using multiple approaches, including filter-bonding analysis, GFP reporter assay, and biolayer interferometry we observed an N protein-panhandle interaction both in vitro and in vivo. The purified WT CCHFV N protein and the stalk domain also recognize the vRNA panhandle of hazara virus, another Nairovirus in the family Bunyaviridae, demonstrating the genus-specific nature ofNprotein-panhandle interaction. Another RNA-binding site was identified at the head domain of CCHFV N protein that nonspecifically recognizes the single strand RNA (ssRNA) of viral or nonviral origin. Expression of CCHFVNprotein stalk domain active in panhandle binding, dramatically inhibited the hazara virus replication in cell culture, illustrating the role of N protein-panhandle interaction in Nairovirus replication. Our findings reveal the stalk domain of N protein as a potential target in therapeutic interventions to manage CCHFV disease. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Biological Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1074/jbc.RA118.004976
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      – Code: eng
        Text: English
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        PageCount: 15
        StartPage: 5023
    Subjects:
      – SubjectFull: Hemorrhagic fever
        Type: general
      – SubjectFull: Viral proteins
        Type: general
      – SubjectFull: Virus diseases
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      – SubjectFull: Protein domains
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      – SubjectFull: Stalking
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      – SubjectFull: Base pairs
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      – TitleFull: Crimean-Congo hemorrhagic fever virus nucleocapsid protein harbors distinct RNA-binding sites in the stalk and head domains.
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              Text: 3/29/2019
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