A simple biosynthetic pathway for 2,3-butanediol production in Thermococcus onnurineus NA1.
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| Title: | A simple biosynthetic pathway for 2,3-butanediol production in Thermococcus onnurineus NA1. |
|---|---|
| Authors: | Lee, Gyu Bi1,2, Kim, Yun Jae1,2 bio1213@kiost.ac.kr, Lim, Jae Kyu1,2, Kim, Tae Wan3, Kang, Sung Gyun1,2, Lee, Hyun Sook1,2, Lee, Jung-Hyun1,2 jlee@kiost.ac.kr |
| Source: | Applied Microbiology & Biotechnology. Apr2019, Vol. 103 Issue 8, p3477-3485. 9p. |
| Subjects: | Butanediol manufacturing, Biosynthesis, Thermophilic archaebacteria, Gene expression, High temperature chemistry |
| Abstract: | The biosynthetic pathway of 2,3-butanediol (2,3-BDO) production from pyruvate under anaerobic conditions includes three enzymes: acetolactate synthase (ALS), acetolactate decarboxylase (ALDC), and acetoin reductase (AR). Recently, in anaerobic hyperthermophilic Pyrococcus furiosus, it has been reported that acetoin, a precursor of 2,3-BDO, is produced from pyruvate by ALS through a temperature-dependent metabolic switch. In this study, we first attempted to produce 2,3-BDO from Thermococcus onnurineus NA1 using a simple biosynthetic pathway by two enzymes (ALS and AR) at a high temperature. Two heterologous genes, acetolactate synthase (alsS) from Pyrococcus sp. NA2 and alcohol dehydrogenase (adh) from T. guaymacensis, were introduced and expressed in T. onnurineus NA1. The mutant strain produced approximately 3.3 mM 2,3-BDO at 80 °C. An acetyl-CoA synthetase IIIα (TON_1001) was further deleted to enhance 2,3-BDO production, and the mutant strain showed a 25% increase in the specific production of 2,3-BDO. Furthermore, when carbon monoxide (CO) gas was added as a reductant, specific production of 2,3-BDO increased by 45%. These results suggest a new biosynthetic pathway for 2,3-BDO and demonstrate the possibility of T. onnurineus NA1 as a platform strain for 2,3-BDO production at high temperatures. [ABSTRACT FROM AUTHOR] |
| Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Header | DbId: egs DbLabel: Engineering Source An: 135752406 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: A simple biosynthetic pathway for 2,3-butanediol production in Thermococcus onnurineus NA1. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Lee%2C+Gyu+Bi%22">Lee, Gyu Bi</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Kim%2C+Yun+Jae%22">Kim, Yun Jae</searchLink><relatesTo>1,2</relatesTo><i> bio1213@kiost.ac.kr</i><br /><searchLink fieldCode="AR" term="%22Lim%2C+Jae+Kyu%22">Lim, Jae Kyu</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Kim%2C+Tae+Wan%22">Kim, Tae Wan</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Kang%2C+Sung+Gyun%22">Kang, Sung Gyun</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Lee%2C+Hyun+Sook%22">Lee, Hyun Sook</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Lee%2C+Jung-Hyun%22">Lee, Jung-Hyun</searchLink><relatesTo>1,2</relatesTo><i> jlee@kiost.ac.kr</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Applied+Microbiology+%26+Biotechnology%22">Applied Microbiology & Biotechnology</searchLink>. Apr2019, Vol. 103 Issue 8, p3477-3485. 9p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Butanediol+manufacturing%22">Butanediol manufacturing</searchLink><br /><searchLink fieldCode="DE" term="%22Biosynthesis%22">Biosynthesis</searchLink><br /><searchLink fieldCode="DE" term="%22Thermophilic+archaebacteria%22">Thermophilic archaebacteria</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink><br /><searchLink fieldCode="DE" term="%22High+temperature+chemistry%22">High temperature chemistry</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The biosynthetic pathway of 2,3-butanediol (2,3-BDO) production from pyruvate under anaerobic conditions includes three enzymes: acetolactate synthase (ALS), acetolactate decarboxylase (ALDC), and acetoin reductase (AR). Recently, in anaerobic hyperthermophilic Pyrococcus furiosus, it has been reported that acetoin, a precursor of 2,3-BDO, is produced from pyruvate by ALS through a temperature-dependent metabolic switch. In this study, we first attempted to produce 2,3-BDO from Thermococcus onnurineus NA1 using a simple biosynthetic pathway by two enzymes (ALS and AR) at a high temperature. Two heterologous genes, acetolactate synthase (alsS) from Pyrococcus sp. NA2 and alcohol dehydrogenase (adh) from T. guaymacensis, were introduced and expressed in T. onnurineus NA1. The mutant strain produced approximately 3.3 mM 2,3-BDO at 80 °C. An acetyl-CoA synthetase IIIα (TON_1001) was further deleted to enhance 2,3-BDO production, and the mutant strain showed a 25% increase in the specific production of 2,3-BDO. Furthermore, when carbon monoxide (CO) gas was added as a reductant, specific production of 2,3-BDO increased by 45%. These results suggest a new biosynthetic pathway for 2,3-BDO and demonstrate the possibility of T. onnurineus NA1 as a platform strain for 2,3-BDO production at high temperatures. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00253-019-09724-z Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 3477 Subjects: – SubjectFull: Butanediol manufacturing Type: general – SubjectFull: Biosynthesis Type: general – SubjectFull: Thermophilic archaebacteria Type: general – SubjectFull: Gene expression Type: general – SubjectFull: High temperature chemistry Type: general Titles: – TitleFull: A simple biosynthetic pathway for 2,3-butanediol production in Thermococcus onnurineus NA1. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Lee, Gyu Bi – PersonEntity: Name: NameFull: Kim, Yun Jae – PersonEntity: Name: NameFull: Lim, Jae Kyu – PersonEntity: Name: NameFull: Kim, Tae Wan – PersonEntity: Name: NameFull: Kang, Sung Gyun – PersonEntity: Name: NameFull: Lee, Hyun Sook – PersonEntity: Name: NameFull: Lee, Jung-Hyun IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 04 Text: Apr2019 Type: published Y: 2019 Identifiers: – Type: issn-print Value: 01757598 Numbering: – Type: volume Value: 103 – Type: issue Value: 8 Titles: – TitleFull: Applied Microbiology & Biotechnology Type: main |
| ResultId | 1 |
