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Fine mapping of the wheat powdery mildew resistance gene Pm52 using comparative genomics analysis and the Chinese Spring reference genomic sequence.

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Title: Fine mapping of the wheat powdery mildew resistance gene Pm52 using comparative genomics analysis and the Chinese Spring reference genomic sequence.
Authors: Wu, Peipei1,2 (AUTHOR), Hu, Jinghuang1 (AUTHOR), Zou, Jingwei1 (AUTHOR), Qiu, Dan1 (AUTHOR), Qu, Yunfeng3 (AUTHOR), Li, Yahui1 (AUTHOR), Li, Teng1 (AUTHOR), Zhang, Hongjun1 (AUTHOR), Yang, Li1 (AUTHOR), Liu, Hongwei1 (AUTHOR), Zhou, Yang1 (AUTHOR), Zhang, Zhongjun2 (AUTHOR), Li, Jingting4 (AUTHOR) jingting_lee@163.com, Liu, Zhiyong5 (AUTHOR) zyliu@genetics.ac.cn, Li, Hongjie1 (AUTHOR) lihongjie@caas.cn
Source: Theoretical & Applied Genetics. May2019, Vol. 132 Issue 5, p1451-1461. 11p.
Subjects: Plant gene mapping, Powdery mildew diseases, Comparative genomics, Wheat, Winter wheat, Comparative studies, Chromosomes
Abstract: Key message: A high-resolution genetic linkage map was constructed using the comparative genomics analysis approach and the wheat reference genome, which placed wheat powdery mildew resistance gene Pm52 in a 0.21-cM genetic interval on chromosome arm 2BL. The gene Pm52 confers resistance to powdery mildew and has been previously mapped on chromosome arm 2BL in winter wheat cultivar Liangxing 99. Because of its effectiveness against the disease, this study was initiated to finely map Pm52 using the comparative genomics analysis approach and the wheat reference genomic sequence. Based on the EST sequences that were located in the chromosome region flanking Pm52, four EST-SSR markers were developed, and another nine SSR markers were developed using the comparative genomics technology. These thirteen markers were integrated into a genetic linkage map using an F2:3 subpopulation of the Liangxing 99 × Zhongzuo 9504 cross. Pm52 was mapped within a 3.2-cM genetic interval in the subpopulation that corresponded to a ~40-Mb genomic interval on chromosome arm 2BL of the Chinese Spring reference genome. The Pm52-flanking markers Xicsl163 and Xicsl62 identified 344 recombinant individuals from 8820 F2 plants. Nine SSR markers generated from the Chinese Spring genomic interval were incorporated into a high-resolution genetic linkage map, which placed Pm52 in a 0.21-cM genetic interval corresponding to 5.6-Mb genomic region. The constructed high-resolution genetic linkage map will facilitate the map-based cloning of Pm52 and its marker-assisted selection. [ABSTRACT FROM AUTHOR]
Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Fine mapping of the wheat powdery mildew resistance gene Pm52 using comparative genomics analysis and the Chinese Spring reference genomic sequence.
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  Data: <searchLink fieldCode="AR" term="%22Wu%2C+Peipei%22">Wu, Peipei</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hu%2C+Jinghuang%22">Hu, Jinghuang</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zou%2C+Jingwei%22">Zou, Jingwei</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Qiu%2C+Dan%22">Qiu, Dan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Qu%2C+Yunfeng%22">Qu, Yunfeng</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Li%2C+Yahui%22">Li, Yahui</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Li%2C+Teng%22">Li, Teng</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Hongjun%22">Zhang, Hongjun</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yang%2C+Li%22">Yang, Li</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Liu%2C+Hongwei%22">Liu, Hongwei</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhou%2C+Yang%22">Zhou, Yang</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Zhongjun%22">Zhang, Zhongjun</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Li%2C+Jingting%22">Li, Jingting</searchLink><relatesTo>4</relatesTo> (AUTHOR)<i> jingting_lee@163.com</i><br /><searchLink fieldCode="AR" term="%22Liu%2C+Zhiyong%22">Liu, Zhiyong</searchLink><relatesTo>5</relatesTo> (AUTHOR)<i> zyliu@genetics.ac.cn</i><br /><searchLink fieldCode="AR" term="%22Li%2C+Hongjie%22">Li, Hongjie</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> lihongjie@caas.cn</i>
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  Data: <searchLink fieldCode="JN" term="%22Theoretical+%26+Applied+Genetics%22">Theoretical & Applied Genetics</searchLink>. May2019, Vol. 132 Issue 5, p1451-1461. 11p.
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  Data: <searchLink fieldCode="DE" term="%22Plant+gene+mapping%22">Plant gene mapping</searchLink><br /><searchLink fieldCode="DE" term="%22Powdery+mildew+diseases%22">Powdery mildew diseases</searchLink><br /><searchLink fieldCode="DE" term="%22Comparative+genomics%22">Comparative genomics</searchLink><br /><searchLink fieldCode="DE" term="%22Wheat%22">Wheat</searchLink><br /><searchLink fieldCode="DE" term="%22Winter+wheat%22">Winter wheat</searchLink><br /><searchLink fieldCode="DE" term="%22Comparative+studies%22">Comparative studies</searchLink><br /><searchLink fieldCode="DE" term="%22Chromosomes%22">Chromosomes</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Key message: A high-resolution genetic linkage map was constructed using the comparative genomics analysis approach and the wheat reference genome, which placed wheat powdery mildew resistance gene Pm52 in a 0.21-cM genetic interval on chromosome arm 2BL. The gene Pm52 confers resistance to powdery mildew and has been previously mapped on chromosome arm 2BL in winter wheat cultivar Liangxing 99. Because of its effectiveness against the disease, this study was initiated to finely map Pm52 using the comparative genomics analysis approach and the wheat reference genomic sequence. Based on the EST sequences that were located in the chromosome region flanking Pm52, four EST-SSR markers were developed, and another nine SSR markers were developed using the comparative genomics technology. These thirteen markers were integrated into a genetic linkage map using an F2:3 subpopulation of the Liangxing 99 × Zhongzuo 9504 cross. Pm52 was mapped within a 3.2-cM genetic interval in the subpopulation that corresponded to a ~40-Mb genomic interval on chromosome arm 2BL of the Chinese Spring reference genome. The Pm52-flanking markers Xicsl163 and Xicsl62 identified 344 recombinant individuals from 8820 F2 plants. Nine SSR markers generated from the Chinese Spring genomic interval were incorporated into a high-resolution genetic linkage map, which placed Pm52 in a 0.21-cM genetic interval corresponding to 5.6-Mb genomic region. The constructed high-resolution genetic linkage map will facilitate the map-based cloning of Pm52 and its marker-assisted selection. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1007/s00122-019-03291-7
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      – Code: eng
        Text: English
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        PageCount: 11
        StartPage: 1451
    Subjects:
      – SubjectFull: Plant gene mapping
        Type: general
      – SubjectFull: Powdery mildew diseases
        Type: general
      – SubjectFull: Comparative genomics
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      – SubjectFull: Wheat
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      – SubjectFull: Winter wheat
        Type: general
      – SubjectFull: Comparative studies
        Type: general
      – SubjectFull: Chromosomes
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      – TitleFull: Fine mapping of the wheat powdery mildew resistance gene Pm52 using comparative genomics analysis and the Chinese Spring reference genomic sequence.
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              Text: May2019
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