Flow injection ionization-tandem mass spectrometry-based estimation of a panel of lysophosphatidylcholines in dried blood spots for screening of X-linked adrenoleukodystrophy.
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| Title: | Flow injection ionization-tandem mass spectrometry-based estimation of a panel of lysophosphatidylcholines in dried blood spots for screening of X-linked adrenoleukodystrophy. |
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| Authors: | Natarajan, Archana1 (AUTHOR), Christopher, Rita1 (AUTHOR) rita@nimhans.ac.in, Netravathi, Manjunath1 (AUTHOR), Bhat, Maya D.1 (AUTHOR), Chandra, Sadanandavalli Retnaswami1 (AUTHOR) |
| Source: | Clinica Chimica Acta. Aug2019, Vol. 495, p167-173. 7p. |
| Subjects: | Federation Internationale de l'Automobile (Organization), Adrenoleukodystrophy, Electrospray ionization mass spectrometry, Mass spectrometry, Mass spectrometers, Blood |
| Abstract: | Elevated blood C26:0 lysophosphatidylcholine (LPC) is a diagnostic marker for X-linked adrenoleukodystrophy (X-ALD). Our aim was to develop a flow injection ionization-tandem mass spectrometry (FIA-MS/MS) method for estimating a panel of LPCs (C20:0-C26:0-LPCs) in dried blood spots (DBS) and to determine the sensitivity and specificity of this method for high-throughput screening for X-ALD. LPCs (C20:0-C26:0) were extracted from 3.2 mm DBS in a 96-well plate, spiked with isotopically-labelled internal standard (C26:0-d4-LPC) and measured by FIA-MS/MS in electrospray ionization (ESI)-positive, multiple reaction monitoring (MRM) mode using a triple quadrupole, tandem mass spectrometer. The sensitivity and specificity of the FIA-MS/MS method for screening of X-ALD was determined. The FIA-MS/MS method was compared with the LC-MS/MS method for estimating LPC concentrations. Elevated C26:0 and C24:0-LPCs were 100% sensitive for identification of X-ALD. However, specificity was only 78.33% for C26:0 and 98.33% for C24:0-LPCs. Sensitivity for C22:0 and C20:0 LPCs were 89.29%, 78.33% and specificity, 67.86% and 73.33%, respectively. The FIA-MS/MS method showed good concordance with the LC-MS/MS method. The FIA-MS/MS method for estimating C26:0 and C24:0-LPCs in DBS is suitable for first-tier screening of newborns for X-ALD. Second-tier confirmatory testing is required to screen positive cases. • Lysophosphatidylcholines (LPCs) were measured from blood spots by FIA-LC-MS/MS method. • Elevated C26:0 and C24:0-LPCs were 100% sensitive for identification of X-ALD. • FIA-MS/MS showed good concordance with LC-MS/MS method. • FIA-MS/MS method can be used for high-throughput, first tier screening for X-ALD. [ABSTRACT FROM AUTHOR] |
| Copyright of Clinica Chimica Acta is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 137072838 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Flow injection ionization-tandem mass spectrometry-based estimation of a panel of lysophosphatidylcholines in dried blood spots for screening of X-linked adrenoleukodystrophy. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Natarajan%2C+Archana%22">Natarajan, Archana</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Christopher%2C+Rita%22">Christopher, Rita</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> rita@nimhans.ac.in</i><br /><searchLink fieldCode="AR" term="%22Netravathi%2C+Manjunath%22">Netravathi, Manjunath</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bhat%2C+Maya+D%2E%22">Bhat, Maya D.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chandra%2C+Sadanandavalli+Retnaswami%22">Chandra, Sadanandavalli Retnaswami</searchLink><relatesTo>1</relatesTo> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Clinica+Chimica+Acta%22">Clinica Chimica Acta</searchLink>. Aug2019, Vol. 495, p167-173. 7p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Federation+Internationale+de+l'Automobile+%28Organization%29%22">Federation Internationale de l'Automobile (Organization)</searchLink><br /><searchLink fieldCode="DE" term="%22Adrenoleukodystrophy%22">Adrenoleukodystrophy</searchLink><br /><searchLink fieldCode="DE" term="%22Electrospray+ionization+mass+spectrometry%22">Electrospray ionization mass spectrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Mass+spectrometry%22">Mass spectrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Mass+spectrometers%22">Mass spectrometers</searchLink><br /><searchLink fieldCode="DE" term="%22Blood%22">Blood</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Elevated blood C26:0 lysophosphatidylcholine (LPC) is a diagnostic marker for X-linked adrenoleukodystrophy (X-ALD). Our aim was to develop a flow injection ionization-tandem mass spectrometry (FIA-MS/MS) method for estimating a panel of LPCs (C20:0-C26:0-LPCs) in dried blood spots (DBS) and to determine the sensitivity and specificity of this method for high-throughput screening for X-ALD. LPCs (C20:0-C26:0) were extracted from 3.2 mm DBS in a 96-well plate, spiked with isotopically-labelled internal standard (C26:0-d4-LPC) and measured by FIA-MS/MS in electrospray ionization (ESI)-positive, multiple reaction monitoring (MRM) mode using a triple quadrupole, tandem mass spectrometer. The sensitivity and specificity of the FIA-MS/MS method for screening of X-ALD was determined. The FIA-MS/MS method was compared with the LC-MS/MS method for estimating LPC concentrations. Elevated C26:0 and C24:0-LPCs were 100% sensitive for identification of X-ALD. However, specificity was only 78.33% for C26:0 and 98.33% for C24:0-LPCs. Sensitivity for C22:0 and C20:0 LPCs were 89.29%, 78.33% and specificity, 67.86% and 73.33%, respectively. The FIA-MS/MS method showed good concordance with the LC-MS/MS method. The FIA-MS/MS method for estimating C26:0 and C24:0-LPCs in DBS is suitable for first-tier screening of newborns for X-ALD. Second-tier confirmatory testing is required to screen positive cases. • Lysophosphatidylcholines (LPCs) were measured from blood spots by FIA-LC-MS/MS method. • Elevated C26:0 and C24:0-LPCs were 100% sensitive for identification of X-ALD. • FIA-MS/MS showed good concordance with LC-MS/MS method. • FIA-MS/MS method can be used for high-throughput, first tier screening for X-ALD. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Clinica Chimica Acta is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.cca.2019.04.059 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 7 StartPage: 167 Subjects: – SubjectFull: Federation Internationale de l'Automobile (Organization) Type: general – SubjectFull: Adrenoleukodystrophy Type: general – SubjectFull: Electrospray ionization mass spectrometry Type: general – SubjectFull: Mass spectrometry Type: general – SubjectFull: Mass spectrometers Type: general – SubjectFull: Blood Type: general Titles: – TitleFull: Flow injection ionization-tandem mass spectrometry-based estimation of a panel of lysophosphatidylcholines in dried blood spots for screening of X-linked adrenoleukodystrophy. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Natarajan, Archana – PersonEntity: Name: NameFull: Christopher, Rita – PersonEntity: Name: NameFull: Netravathi, Manjunath – PersonEntity: Name: NameFull: Bhat, Maya D. – PersonEntity: Name: NameFull: Chandra, Sadanandavalli Retnaswami IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 08 Text: Aug2019 Type: published Y: 2019 Identifiers: – Type: issn-print Value: 00098981 Numbering: – Type: volume Value: 495 Titles: – TitleFull: Clinica Chimica Acta Type: main |
| ResultId | 1 |
