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Flow injection ionization-tandem mass spectrometry-based estimation of a panel of lysophosphatidylcholines in dried blood spots for screening of X-linked adrenoleukodystrophy.

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Title: Flow injection ionization-tandem mass spectrometry-based estimation of a panel of lysophosphatidylcholines in dried blood spots for screening of X-linked adrenoleukodystrophy.
Authors: Natarajan, Archana1 (AUTHOR), Christopher, Rita1 (AUTHOR) rita@nimhans.ac.in, Netravathi, Manjunath1 (AUTHOR), Bhat, Maya D.1 (AUTHOR), Chandra, Sadanandavalli Retnaswami1 (AUTHOR)
Source: Clinica Chimica Acta. Aug2019, Vol. 495, p167-173. 7p.
Subjects: Federation Internationale de l'Automobile (Organization), Adrenoleukodystrophy, Electrospray ionization mass spectrometry, Mass spectrometry, Mass spectrometers, Blood
Abstract: Elevated blood C26:0 lysophosphatidylcholine (LPC) is a diagnostic marker for X-linked adrenoleukodystrophy (X-ALD). Our aim was to develop a flow injection ionization-tandem mass spectrometry (FIA-MS/MS) method for estimating a panel of LPCs (C20:0-C26:0-LPCs) in dried blood spots (DBS) and to determine the sensitivity and specificity of this method for high-throughput screening for X-ALD. LPCs (C20:0-C26:0) were extracted from 3.2 mm DBS in a 96-well plate, spiked with isotopically-labelled internal standard (C26:0-d4-LPC) and measured by FIA-MS/MS in electrospray ionization (ESI)-positive, multiple reaction monitoring (MRM) mode using a triple quadrupole, tandem mass spectrometer. The sensitivity and specificity of the FIA-MS/MS method for screening of X-ALD was determined. The FIA-MS/MS method was compared with the LC-MS/MS method for estimating LPC concentrations. Elevated C26:0 and C24:0-LPCs were 100% sensitive for identification of X-ALD. However, specificity was only 78.33% for C26:0 and 98.33% for C24:0-LPCs. Sensitivity for C22:0 and C20:0 LPCs were 89.29%, 78.33% and specificity, 67.86% and 73.33%, respectively. The FIA-MS/MS method showed good concordance with the LC-MS/MS method. The FIA-MS/MS method for estimating C26:0 and C24:0-LPCs in DBS is suitable for first-tier screening of newborns for X-ALD. Second-tier confirmatory testing is required to screen positive cases. • Lysophosphatidylcholines (LPCs) were measured from blood spots by FIA-LC-MS/MS method. • Elevated C26:0 and C24:0-LPCs were 100% sensitive for identification of X-ALD. • FIA-MS/MS showed good concordance with LC-MS/MS method. • FIA-MS/MS method can be used for high-throughput, first tier screening for X-ALD. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
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Abstract:Elevated blood C26:0 lysophosphatidylcholine (LPC) is a diagnostic marker for X-linked adrenoleukodystrophy (X-ALD). Our aim was to develop a flow injection ionization-tandem mass spectrometry (FIA-MS/MS) method for estimating a panel of LPCs (C20:0-C26:0-LPCs) in dried blood spots (DBS) and to determine the sensitivity and specificity of this method for high-throughput screening for X-ALD. LPCs (C20:0-C26:0) were extracted from 3.2 mm DBS in a 96-well plate, spiked with isotopically-labelled internal standard (C26:0-d4-LPC) and measured by FIA-MS/MS in electrospray ionization (ESI)-positive, multiple reaction monitoring (MRM) mode using a triple quadrupole, tandem mass spectrometer. The sensitivity and specificity of the FIA-MS/MS method for screening of X-ALD was determined. The FIA-MS/MS method was compared with the LC-MS/MS method for estimating LPC concentrations. Elevated C26:0 and C24:0-LPCs were 100% sensitive for identification of X-ALD. However, specificity was only 78.33% for C26:0 and 98.33% for C24:0-LPCs. Sensitivity for C22:0 and C20:0 LPCs were 89.29%, 78.33% and specificity, 67.86% and 73.33%, respectively. The FIA-MS/MS method showed good concordance with the LC-MS/MS method. The FIA-MS/MS method for estimating C26:0 and C24:0-LPCs in DBS is suitable for first-tier screening of newborns for X-ALD. Second-tier confirmatory testing is required to screen positive cases. • Lysophosphatidylcholines (LPCs) were measured from blood spots by FIA-LC-MS/MS method. • Elevated C26:0 and C24:0-LPCs were 100% sensitive for identification of X-ALD. • FIA-MS/MS showed good concordance with LC-MS/MS method. • FIA-MS/MS method can be used for high-throughput, first tier screening for X-ALD. [ABSTRACT FROM AUTHOR]
ISSN:00098981
DOI:10.1016/j.cca.2019.04.059