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Comparison of two different laser photobiomodulation protocols on the viability of random skin flap in rats.

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Title: Comparison of two different laser photobiomodulation protocols on the viability of random skin flap in rats.
Authors: Martignago, Cintia Cristina Santi1 csantimartignago@yahoo.com.br, Tim, C. R.2,3, Assis, L.2,3, Neves, L. M. G.1, Bossini, P. S.4, Renno, A. C.2, Avo, L. R. S.1, Liebano, R. E.1, Parizotto, N. A.1,3
Source: Lasers in Medical Science. Jul2019, Vol. 34 Issue 5, p1041-1047. 7p.
Subjects: Dermatologic surgery, Laser surgery, Surgical flaps, Laboratory rats, Medical protocols, Evaluation research, Phenomenological biology, Lasers, Necrosis, Skin, Fibroblasts, Rats, Cell nuclei, Growth factors, Animal experimentation, Research methodology, Research, Collagen, Comparative studies, Metabolism
Abstract: To identify the best low level laser photobiomodulation application site at the same irradiation time to increase the viability of the skin flap in rats. Eighteen male rats (Rattus norvegicus: var. Albinus, Rodentia Mammalia) were randomly distributed into three groups (n = 6). Group I (GI) was submitted to simulated laser photobiomodulation; group II (GII) was submitted to laser photobiomodulation at three points in the flap cranial base, and group III (GIII) was submitted to laser photobiomodulation at 12 points distributed along the flap. All groups were irradiated with an Indium, Galium, Aluminum, and Phosphorus diode laser (InGaAlP), 660 nm, with 50 mW power, irradiated for a total time of 240 s in continuous emission mode. The treatment started immediately after performing the cranial base random skin flap (10 × 4 cm2 dimension) and reapplied every 24 h, with a total of five applications. The animals were euthanized after the evaluation of the percentage of necrosis area, and the material was collected for histological analysis on the seventh postoperative day. GII animals presented a statistically significant decrease for the necrosis area when compared to the other groups, and a statistically significant increase in the quantification of collagen when compared to the control. We did not observe a statistical difference between the TGFβ and FGF expression in the different groups evaluated. The application of laser photobiomodulation at three points of the flap cranial base was more effective than at 12 points regarding the reduction of necrosis area. [ABSTRACT FROM AUTHOR]
Copyright of Lasers in Medical Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Comparison of two different laser photobiomodulation protocols on the viability of random skin flap in rats.
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  Data: <searchLink fieldCode="AR" term="%22Martignago%2C+Cintia+Cristina+Santi%22">Martignago, Cintia Cristina Santi</searchLink><relatesTo>1</relatesTo><i> csantimartignago@yahoo.com.br</i><br /><searchLink fieldCode="AR" term="%22Tim%2C+C%2E+R%2E%22">Tim, C. R.</searchLink><relatesTo>2,3</relatesTo><br /><searchLink fieldCode="AR" term="%22Assis%2C+L%2E%22">Assis, L.</searchLink><relatesTo>2,3</relatesTo><br /><searchLink fieldCode="AR" term="%22Neves%2C+L%2E+M%2E+G%2E%22">Neves, L. M. G.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Bossini%2C+P%2E+S%2E%22">Bossini, P. S.</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Renno%2C+A%2E+C%2E%22">Renno, A. C.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Avo%2C+L%2E+R%2E+S%2E%22">Avo, L. R. S.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Liebano%2C+R%2E+E%2E%22">Liebano, R. E.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Parizotto%2C+N%2E+A%2E%22">Parizotto, N. A.</searchLink><relatesTo>1,3</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Lasers+in+Medical+Science%22">Lasers in Medical Science</searchLink>. Jul2019, Vol. 34 Issue 5, p1041-1047. 7p.
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  Data: <searchLink fieldCode="DE" term="%22Dermatologic+surgery%22">Dermatologic surgery</searchLink><br /><searchLink fieldCode="DE" term="%22Laser+surgery%22">Laser surgery</searchLink><br /><searchLink fieldCode="DE" term="%22Surgical+flaps%22">Surgical flaps</searchLink><br /><searchLink fieldCode="DE" term="%22Laboratory+rats%22">Laboratory rats</searchLink><br /><searchLink fieldCode="DE" term="%22Medical+protocols%22">Medical protocols</searchLink><br /><searchLink fieldCode="DE" term="%22Evaluation+research%22">Evaluation research</searchLink><br /><searchLink fieldCode="DE" term="%22Phenomenological+biology%22">Phenomenological biology</searchLink><br /><searchLink fieldCode="DE" term="%22Lasers%22">Lasers</searchLink><br /><searchLink fieldCode="DE" term="%22Necrosis%22">Necrosis</searchLink><br /><searchLink fieldCode="DE" term="%22Skin%22">Skin</searchLink><br /><searchLink fieldCode="DE" term="%22Fibroblasts%22">Fibroblasts</searchLink><br /><searchLink fieldCode="DE" term="%22Rats%22">Rats</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+nuclei%22">Cell nuclei</searchLink><br /><searchLink fieldCode="DE" term="%22Growth+factors%22">Growth factors</searchLink><br /><searchLink fieldCode="DE" term="%22Animal+experimentation%22">Animal experimentation</searchLink><br /><searchLink fieldCode="DE" term="%22Research+methodology%22">Research methodology</searchLink><br /><searchLink fieldCode="DE" term="%22Research%22">Research</searchLink><br /><searchLink fieldCode="DE" term="%22Collagen%22">Collagen</searchLink><br /><searchLink fieldCode="DE" term="%22Comparative+studies%22">Comparative studies</searchLink><br /><searchLink fieldCode="DE" term="%22Metabolism%22">Metabolism</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: To identify the best low level laser photobiomodulation application site at the same irradiation time to increase the viability of the skin flap in rats. Eighteen male rats (Rattus norvegicus: var. Albinus, Rodentia Mammalia) were randomly distributed into three groups (n = 6). Group I (GI) was submitted to simulated laser photobiomodulation; group II (GII) was submitted to laser photobiomodulation at three points in the flap cranial base, and group III (GIII) was submitted to laser photobiomodulation at 12 points distributed along the flap. All groups were irradiated with an Indium, Galium, Aluminum, and Phosphorus diode laser (InGaAlP), 660 nm, with 50 mW power, irradiated for a total time of 240 s in continuous emission mode. The treatment started immediately after performing the cranial base random skin flap (10 × 4 cm2 dimension) and reapplied every 24 h, with a total of five applications. The animals were euthanized after the evaluation of the percentage of necrosis area, and the material was collected for histological analysis on the seventh postoperative day. GII animals presented a statistically significant decrease for the necrosis area when compared to the other groups, and a statistically significant increase in the quantification of collagen when compared to the control. We did not observe a statistical difference between the TGFβ and FGF expression in the different groups evaluated. The application of laser photobiomodulation at three points of the flap cranial base was more effective than at 12 points regarding the reduction of necrosis area. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of Lasers in Medical Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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    Identifiers:
      – Type: doi
        Value: 10.1007/s10103-018-2694-3
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      – Code: eng
        Text: English
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        StartPage: 1041
    Subjects:
      – SubjectFull: Dermatologic surgery
        Type: general
      – SubjectFull: Laser surgery
        Type: general
      – SubjectFull: Surgical flaps
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      – SubjectFull: Laboratory rats
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      – SubjectFull: Medical protocols
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      – SubjectFull: Evaluation research
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      – SubjectFull: Phenomenological biology
        Type: general
      – SubjectFull: Lasers
        Type: general
      – SubjectFull: Necrosis
        Type: general
      – SubjectFull: Skin
        Type: general
      – SubjectFull: Fibroblasts
        Type: general
      – SubjectFull: Rats
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      – SubjectFull: Cell nuclei
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      – SubjectFull: Animal experimentation
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        Type: general
      – SubjectFull: Research
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      – SubjectFull: Collagen
        Type: general
      – SubjectFull: Comparative studies
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      – SubjectFull: Metabolism
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              Text: Jul2019
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