Label-free detection of residual breast cancer after neoadjuvant chemotherapy using biomedical multiphoton microscopy.
Saved in:
| Title: | Label-free detection of residual breast cancer after neoadjuvant chemotherapy using biomedical multiphoton microscopy. |
|---|---|
| Authors: | Han, Zhonghua1, Li, Lianhuang2 lhli@fjnu.edu.cn, Kang, Deyong3, Zhan, Zhenlin2, Tu, Haohua4, Wang, Chuan1, Chen, Jianxin2 |
| Source: | Lasers in Medical Science. Oct2019, Vol. 34 Issue 8, p1595-1601. 7p. |
| Subjects: | Breast cancer treatment, Cancer chemotherapy, Multiphoton excitation microscopy, Cell nuclei, Surgeons, Breast tumor diagnosis, Research funding, Breast tumors, Combined modality therapy, Microscopy, Carcinogenesis, Stains & staining (Microscopy), Tumor classification, Breast |
| Abstract: | Neoadjuvant chemotherapy has become a standard treatment for breast cancer as it has been shown to increase the rate of breast preservation and to improve outcome in patients. However, how to accurately detect residual tumors is still a challenge. In this work, we tried to use multiphoton imaging to look for residual breast tumors after preoperative therapy. Imaging results demonstrate that multiphoton microscopy can identify remaining tumor tissues and can even detect rarely residual tumor cells, which would be helpful for surgeons to accurately assess the surgical margin in real time to confirm negative margins during operation. We also performed a quantification analysis of the nuclear area of tumor cells before and after treatment with neoadjuvant chemotherapy. The measurement data show that the tumor cell nuclei after chemotherapy are significantly larger than those without treatment, and there is a statistically significant difference in the nuclear areas between the pre-treatment and post-treatment mammary carcinoma. Our pilot study indicates the potential utility of multiphoton imaging for detecting residual breast carcinoma tissues in fresh, ex vivo specimens without the use of exogenous contrast agents. We foresee real-time intraoperative applications of multiphoton microscopy in evaluating therapy response, and thereby helping clinicians develop individualized treatment plans. [ABSTRACT FROM AUTHOR] |
| Copyright of Lasers in Medical Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
|
Full text is not displayed to guests.
Login for full access.
|
|
| FullText | Links: – Type: pdflink Text: Availability: 1 |
|---|---|
| Header | DbId: egs DbLabel: Engineering Source An: 138812146 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Label-free detection of residual breast cancer after neoadjuvant chemotherapy using biomedical multiphoton microscopy. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Han%2C+Zhonghua%22">Han, Zhonghua</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Li%2C+Lianhuang%22">Li, Lianhuang</searchLink><relatesTo>2</relatesTo><i> lhli@fjnu.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Kang%2C+Deyong%22">Kang, Deyong</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Zhan%2C+Zhenlin%22">Zhan, Zhenlin</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Tu%2C+Haohua%22">Tu, Haohua</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Wang%2C+Chuan%22">Wang, Chuan</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Chen%2C+Jianxin%22">Chen, Jianxin</searchLink><relatesTo>2</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Lasers+in+Medical+Science%22">Lasers in Medical Science</searchLink>. Oct2019, Vol. 34 Issue 8, p1595-1601. 7p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Breast+cancer+treatment%22">Breast cancer treatment</searchLink><br /><searchLink fieldCode="DE" term="%22Cancer+chemotherapy%22">Cancer chemotherapy</searchLink><br /><searchLink fieldCode="DE" term="%22Multiphoton+excitation+microscopy%22">Multiphoton excitation microscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+nuclei%22">Cell nuclei</searchLink><br /><searchLink fieldCode="DE" term="%22Surgeons%22">Surgeons</searchLink><br /><searchLink fieldCode="DE" term="%22Breast+tumor+diagnosis%22">Breast tumor diagnosis</searchLink><br /><searchLink fieldCode="DE" term="%22Research+funding%22">Research funding</searchLink><br /><searchLink fieldCode="DE" term="%22Breast+tumors%22">Breast tumors</searchLink><br /><searchLink fieldCode="DE" term="%22Combined+modality+therapy%22">Combined modality therapy</searchLink><br /><searchLink fieldCode="DE" term="%22Microscopy%22">Microscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Carcinogenesis%22">Carcinogenesis</searchLink><br /><searchLink fieldCode="DE" term="%22Stains+%26+staining+%28Microscopy%29%22">Stains & staining (Microscopy)</searchLink><br /><searchLink fieldCode="DE" term="%22Tumor+classification%22">Tumor classification</searchLink><br /><searchLink fieldCode="DE" term="%22Breast%22">Breast</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Neoadjuvant chemotherapy has become a standard treatment for breast cancer as it has been shown to increase the rate of breast preservation and to improve outcome in patients. However, how to accurately detect residual tumors is still a challenge. In this work, we tried to use multiphoton imaging to look for residual breast tumors after preoperative therapy. Imaging results demonstrate that multiphoton microscopy can identify remaining tumor tissues and can even detect rarely residual tumor cells, which would be helpful for surgeons to accurately assess the surgical margin in real time to confirm negative margins during operation. We also performed a quantification analysis of the nuclear area of tumor cells before and after treatment with neoadjuvant chemotherapy. The measurement data show that the tumor cell nuclei after chemotherapy are significantly larger than those without treatment, and there is a statistically significant difference in the nuclear areas between the pre-treatment and post-treatment mammary carcinoma. Our pilot study indicates the potential utility of multiphoton imaging for detecting residual breast carcinoma tissues in fresh, ex vivo specimens without the use of exogenous contrast agents. We foresee real-time intraoperative applications of multiphoton microscopy in evaluating therapy response, and thereby helping clinicians develop individualized treatment plans. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Lasers in Medical Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=138812146 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s10103-019-02754-z Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 7 StartPage: 1595 Subjects: – SubjectFull: Breast cancer treatment Type: general – SubjectFull: Cancer chemotherapy Type: general – SubjectFull: Multiphoton excitation microscopy Type: general – SubjectFull: Cell nuclei Type: general – SubjectFull: Surgeons Type: general – SubjectFull: Breast tumor diagnosis Type: general – SubjectFull: Research funding Type: general – SubjectFull: Breast tumors Type: general – SubjectFull: Combined modality therapy Type: general – SubjectFull: Microscopy Type: general – SubjectFull: Carcinogenesis Type: general – SubjectFull: Stains & staining (Microscopy) Type: general – SubjectFull: Tumor classification Type: general – SubjectFull: Breast Type: general Titles: – TitleFull: Label-free detection of residual breast cancer after neoadjuvant chemotherapy using biomedical multiphoton microscopy. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Han, Zhonghua – PersonEntity: Name: NameFull: Li, Lianhuang – PersonEntity: Name: NameFull: Kang, Deyong – PersonEntity: Name: NameFull: Zhan, Zhenlin – PersonEntity: Name: NameFull: Tu, Haohua – PersonEntity: Name: NameFull: Wang, Chuan – PersonEntity: Name: NameFull: Chen, Jianxin IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 10 Text: Oct2019 Type: published Y: 2019 Identifiers: – Type: issn-print Value: 02688921 Numbering: – Type: volume Value: 34 – Type: issue Value: 8 Titles: – TitleFull: Lasers in Medical Science Type: main |
| ResultId | 1 |
