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Influence of brain gangliosides on the formation and properties of supported lipid bilayers.

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Title: Influence of brain gangliosides on the formation and properties of supported lipid bilayers.
Authors: Jordan, Luke R.1 (AUTHOR), Blauch, Megan E.1 (AUTHOR), Baxter, Ashley M.1 (AUTHOR), Cawley, Jennie L.1 (AUTHOR), Wittenberg, Nathan J.1 (AUTHOR) njw@lehigh.edu
Source: Colloids & Surfaces B: Biointerfaces. Nov2019, Vol. 183, pN.PAG-N.PAG. 1p.
Subjects: Gangliosides, Glycolipids, Bilayer lipid membranes, Quartz crystal microbalances, Sialic acids, Membrane lipids, Cell membranes
Abstract: • Gangliosides significantly slow the supported lipid bilayer (SLB) formation process. • Terminal sialic acids slow process more than internal sialic acids. • Calcium speeds up sluggish SLB formation and helps form complete bilayers. • Lipid diffusion in membranes slows with increased ganglioside content. • Binding kinetics of antibodies to gangliosides in SLBs are measured with QCM-D. Gangliosides are glycolipids that are enriched on the outer surface of cell membranes. Gangliosides are receptors for a number of signaling molecules and toxins, and therefore are often incorporated into biosensors. Many of these biosensors incorporate gangliosides into supported lipid bilayers which are formed by the spontaneous rupture of unilamellar vesicles on glass or SiO 2 substrates. In this work, we used quartz crystal microbalance with dissipation monitoring (QCM-D) to investigate how the presence of the four major brain gangliosides (GM1, GD1a, GD1b, and GT1b) influences the process of supported lipid bilayer formation on SiO 2 surfaces. We show that the rate of supported bilayer formation is dependent on both the charge and position of sialic acid moieties on ganglioside molecules. Additionally, Ca2+ can accelerate ganglioside-rich supported bilayer formation, but the degree of acceleration differs for vesicles containing different gangliosides. Fluorescence recovery after photobleaching measurements show that the presence of all gangliosides reduces lipid diffusion coefficients in a concentration-dependent manner, and that Ca2+ slows lipid diffusion in membranes with and without gangliosides. Finally, we use ganglioside-rich supported bilayers to measure binding constants for a GD1a-binding antibody that has similar properties to antibodies present in a variant of Guillain-Barré syndrome. [ABSTRACT FROM AUTHOR]
Copyright of Colloids & Surfaces B: Biointerfaces is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 139250083
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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Items – Name: Title
  Label: Title
  Group: Ti
  Data: Influence of brain gangliosides on the formation and properties of supported lipid bilayers.
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  Data: <searchLink fieldCode="AR" term="%22Jordan%2C+Luke+R%2E%22">Jordan, Luke R.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Blauch%2C+Megan+E%2E%22">Blauch, Megan E.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Baxter%2C+Ashley+M%2E%22">Baxter, Ashley M.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Cawley%2C+Jennie+L%2E%22">Cawley, Jennie L.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wittenberg%2C+Nathan+J%2E%22">Wittenberg, Nathan J.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> njw@lehigh.edu</i>
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  Data: <searchLink fieldCode="JN" term="%22Colloids+%26+Surfaces+B%3A+Biointerfaces%22">Colloids & Surfaces B: Biointerfaces</searchLink>. Nov2019, Vol. 183, pN.PAG-N.PAG. 1p.
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  Data: <searchLink fieldCode="DE" term="%22Gangliosides%22">Gangliosides</searchLink><br /><searchLink fieldCode="DE" term="%22Glycolipids%22">Glycolipids</searchLink><br /><searchLink fieldCode="DE" term="%22Bilayer+lipid+membranes%22">Bilayer lipid membranes</searchLink><br /><searchLink fieldCode="DE" term="%22Quartz+crystal+microbalances%22">Quartz crystal microbalances</searchLink><br /><searchLink fieldCode="DE" term="%22Sialic+acids%22">Sialic acids</searchLink><br /><searchLink fieldCode="DE" term="%22Membrane+lipids%22">Membrane lipids</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+membranes%22">Cell membranes</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: • Gangliosides significantly slow the supported lipid bilayer (SLB) formation process. • Terminal sialic acids slow process more than internal sialic acids. • Calcium speeds up sluggish SLB formation and helps form complete bilayers. • Lipid diffusion in membranes slows with increased ganglioside content. • Binding kinetics of antibodies to gangliosides in SLBs are measured with QCM-D. Gangliosides are glycolipids that are enriched on the outer surface of cell membranes. Gangliosides are receptors for a number of signaling molecules and toxins, and therefore are often incorporated into biosensors. Many of these biosensors incorporate gangliosides into supported lipid bilayers which are formed by the spontaneous rupture of unilamellar vesicles on glass or SiO 2 substrates. In this work, we used quartz crystal microbalance with dissipation monitoring (QCM-D) to investigate how the presence of the four major brain gangliosides (GM1, GD1a, GD1b, and GT1b) influences the process of supported lipid bilayer formation on SiO 2 surfaces. We show that the rate of supported bilayer formation is dependent on both the charge and position of sialic acid moieties on ganglioside molecules. Additionally, Ca2+ can accelerate ganglioside-rich supported bilayer formation, but the degree of acceleration differs for vesicles containing different gangliosides. Fluorescence recovery after photobleaching measurements show that the presence of all gangliosides reduces lipid diffusion coefficients in a concentration-dependent manner, and that Ca2+ slows lipid diffusion in membranes with and without gangliosides. Finally, we use ganglioside-rich supported bilayers to measure binding constants for a GD1a-binding antibody that has similar properties to antibodies present in a variant of Guillain-Barré syndrome. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Colloids & Surfaces B: Biointerfaces is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.colsurfb.2019.110442
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 1
        StartPage: N.PAG
    Subjects:
      – SubjectFull: Gangliosides
        Type: general
      – SubjectFull: Glycolipids
        Type: general
      – SubjectFull: Bilayer lipid membranes
        Type: general
      – SubjectFull: Quartz crystal microbalances
        Type: general
      – SubjectFull: Sialic acids
        Type: general
      – SubjectFull: Membrane lipids
        Type: general
      – SubjectFull: Cell membranes
        Type: general
    Titles:
      – TitleFull: Influence of brain gangliosides on the formation and properties of supported lipid bilayers.
        Type: main
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      – PersonEntity:
          Name:
            NameFull: Jordan, Luke R.
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            NameFull: Blauch, Megan E.
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            NameFull: Baxter, Ashley M.
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            NameFull: Cawley, Jennie L.
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            NameFull: Wittenberg, Nathan J.
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          Dates:
            – D: 01
              M: 11
              Text: Nov2019
              Type: published
              Y: 2019
          Identifiers:
            – Type: issn-print
              Value: 09277765
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            – Type: volume
              Value: 183
          Titles:
            – TitleFull: Colloids & Surfaces B: Biointerfaces
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