Concentration-driven reverse membrane bioreactor for the fermentation of highly inhibitory lignocellulosic hydrolysate.
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| Title: | Concentration-driven reverse membrane bioreactor for the fermentation of highly inhibitory lignocellulosic hydrolysate. |
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| Authors: | Mahboubi, Amir1,2 (AUTHOR) amir.mahboubi_soufiani@hb.se, Elyasi, Shilan3 (AUTHOR), Doyen, Wim4 (AUTHOR), De Wever, Heleen2 (AUTHOR), Taherzadeh, Mohammad J.1 (AUTHOR) |
| Source: | Process Biochemistry. May2020, Vol. 92, p409-416. 8p. |
| Subjects: | Fermentation, Furfural, Wheat straw, Lignocellulose, Sugar, Ethanol as fuel |
| Abstract: | • Reverse membrane bioreactor was used for fermentation of wheat straw hydrolysate. • Diffusion cell was used to measure the diffusion rate of medium compounds through the membrane. • Membrane-confined yeast cells could detoxify a medium containing up to 16 g/l furfural. • High sugar co-utilization and ethanol yield was achieved in rMBR up to 9 g/l of initial furfural. • The inhibitor tolerance threshold was reached in rMBR with high furfural to cell ratio of 9.5:1. Optimal production of lignocellulosic bioethanol is hindered due to commonly faced issues with the presence of inhibitory compounds and sequentially consumed sugars in the lignocellulosic hydrolysate. Therefore, in order to find a robust fermentation approach, this study aimed at enhancing simultaneous co-assimilation of sugars, and inhibitor tolerance and detoxification. Therefore, fermentation of toxic wheat straw hydrolysate containing up to 20 g/l furfural, using the concentration-driven diffusion-based technique of reverse membrane bioreactor (rMBR) was studied. The rMBR fermentation of the hydrolysate led to complete furfural detoxification and the conversion of 87 % of sugars into ethanol at a yield of 0.48 g/g. Moreover, when the toxicity level of the hydrolysate was increased to 9 g/l of initial furfural, the system responded exceptionally by reducing 89 % of the inhibitor while only experiencing about 25 % drop in the ethanol yield. In addition, using this diffusion-based set-up in extremely inhibitory conditions (16 g/l furfural), cells could detoxify 40 % of the furfural at a high initial furfural to cell ratio of 9.5:1. The rMBR set-up applied proved that by properly synchronizing the medium condition, membrane area, and inhibitor to cell ratio, some of the shortcomings with conventional lignocellulosic fermentation can be tackled, guaranteeing a robust fermentation. [ABSTRACT FROM AUTHOR] |
| Copyright of Process Biochemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 142536364 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Concentration-driven reverse membrane bioreactor for the fermentation of highly inhibitory lignocellulosic hydrolysate. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Mahboubi%2C+Amir%22">Mahboubi, Amir</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> amir.mahboubi_soufiani@hb.se</i><br /><searchLink fieldCode="AR" term="%22Elyasi%2C+Shilan%22">Elyasi, Shilan</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Doyen%2C+Wim%22">Doyen, Wim</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22De+Wever%2C+Heleen%22">De Wever, Heleen</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Taherzadeh%2C+Mohammad+J%2E%22">Taherzadeh, Mohammad J.</searchLink><relatesTo>1</relatesTo> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Process+Biochemistry%22">Process Biochemistry</searchLink>. May2020, Vol. 92, p409-416. 8p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Fermentation%22">Fermentation</searchLink><br /><searchLink fieldCode="DE" term="%22Furfural%22">Furfural</searchLink><br /><searchLink fieldCode="DE" term="%22Wheat+straw%22">Wheat straw</searchLink><br /><searchLink fieldCode="DE" term="%22Lignocellulose%22">Lignocellulose</searchLink><br /><searchLink fieldCode="DE" term="%22Sugar%22">Sugar</searchLink><br /><searchLink fieldCode="DE" term="%22Ethanol+as+fuel%22">Ethanol as fuel</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: • Reverse membrane bioreactor was used for fermentation of wheat straw hydrolysate. • Diffusion cell was used to measure the diffusion rate of medium compounds through the membrane. • Membrane-confined yeast cells could detoxify a medium containing up to 16 g/l furfural. • High sugar co-utilization and ethanol yield was achieved in rMBR up to 9 g/l of initial furfural. • The inhibitor tolerance threshold was reached in rMBR with high furfural to cell ratio of 9.5:1. Optimal production of lignocellulosic bioethanol is hindered due to commonly faced issues with the presence of inhibitory compounds and sequentially consumed sugars in the lignocellulosic hydrolysate. Therefore, in order to find a robust fermentation approach, this study aimed at enhancing simultaneous co-assimilation of sugars, and inhibitor tolerance and detoxification. Therefore, fermentation of toxic wheat straw hydrolysate containing up to 20 g/l furfural, using the concentration-driven diffusion-based technique of reverse membrane bioreactor (rMBR) was studied. The rMBR fermentation of the hydrolysate led to complete furfural detoxification and the conversion of 87 % of sugars into ethanol at a yield of 0.48 g/g. Moreover, when the toxicity level of the hydrolysate was increased to 9 g/l of initial furfural, the system responded exceptionally by reducing 89 % of the inhibitor while only experiencing about 25 % drop in the ethanol yield. In addition, using this diffusion-based set-up in extremely inhibitory conditions (16 g/l furfural), cells could detoxify 40 % of the furfural at a high initial furfural to cell ratio of 9.5:1. The rMBR set-up applied proved that by properly synchronizing the medium condition, membrane area, and inhibitor to cell ratio, some of the shortcomings with conventional lignocellulosic fermentation can be tackled, guaranteeing a robust fermentation. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Process Biochemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.procbio.2020.01.031 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 8 StartPage: 409 Subjects: – SubjectFull: Fermentation Type: general – SubjectFull: Furfural Type: general – SubjectFull: Wheat straw Type: general – SubjectFull: Lignocellulose Type: general – SubjectFull: Sugar Type: general – SubjectFull: Ethanol as fuel Type: general Titles: – TitleFull: Concentration-driven reverse membrane bioreactor for the fermentation of highly inhibitory lignocellulosic hydrolysate. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Mahboubi, Amir – PersonEntity: Name: NameFull: Elyasi, Shilan – PersonEntity: Name: NameFull: Doyen, Wim – PersonEntity: Name: NameFull: De Wever, Heleen – PersonEntity: Name: NameFull: Taherzadeh, Mohammad J. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 05 Text: May2020 Type: published Y: 2020 Identifiers: – Type: issn-print Value: 13595113 Numbering: – Type: volume Value: 92 Titles: – TitleFull: Process Biochemistry Type: main |
| ResultId | 1 |
