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Effects of photobiomodulation therapy in the integration of skin graft in rats.

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Title: Effects of photobiomodulation therapy in the integration of skin graft in rats.
Authors: Castro, Tatiane Neiva Santos1, Martignago, Cintia Cristina Santi2, Assis, Lívia1, de Alexandria, Francisco Eugênio Deusdará1, Rocha, José Carlos Tatmatsu3, Parizotto, Nivaldo Antonio1, Tim, Carla Roberta1 carlinha_tim@hotmail.com
Source: Lasers in Medical Science. Jun2020, Vol. 35 Issue 4, p939-947. 9p.
Subjects: Skin grafting, Animal models in research, Histopathology, Clinical trials, Immunohistochemistry, Interleukins, Collagen, Skin, Cytometry, Animal experimentation, Rats, Mast cells, Animals, Metabolism
Abstract: Skin graft is one of the most common techniques used in plastic surgery and repair. However, there are some complications that can lead to loss of the skin graft. Thus, several features have been studied with the aim of promoting the integration of skin grafts. Among these resources, the use of laser photobiomodulation (laser PBM) has been highlighted. The present study aimed to investigate the effects of laser PBM on the viability and integration of skin grafts in rats. Twenty male Wistar rats (± 250 g) were randomly assigned into two experimental groups with 10 animals each: control group, animals submitted to skin graft and simulation of laser PBM; laser PBM group, submitted to the skin graft and submitted to laser PBM at 660 nm, 40 mW, 60 s, 2.4 J. The animals were submitted to laser photobiomodulation immediately after the surgical procedure and each 24 h. Animal euthanasia occurred on the 7th day after surgery, 24 h after the last treatment session. The histopathological analysis revealed that the laser PBM showed better adhesion of the graft when compared to the control group. Likewise, the morphometric analysis of mast cells, blood vessels, and collagen showed a statistically significant increase in the animals irradiated with the laser PBM when compared to the control group. In addition, immunohistochemical analysis demonstrated that the laser PBM showed statistically higher immunoexpression of FGF when compared to the CG. However, IL-4 immunoexpression did not show statistical difference between the experimental groups. From the results obtained in the present study, it can be suggested that laser photobiomodulation was effective in promoting the integration and viability of total skin grafts in rats. [ABSTRACT FROM AUTHOR]
Copyright of Lasers in Medical Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Effects of photobiomodulation therapy in the integration of skin graft in rats.
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  Data: <searchLink fieldCode="AR" term="%22Castro%2C+Tatiane+Neiva+Santos%22">Castro, Tatiane Neiva Santos</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Martignago%2C+Cintia+Cristina+Santi%22">Martignago, Cintia Cristina Santi</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Assis%2C+Lívia%22">Assis, Lívia</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22de+Alexandria%2C+Francisco+Eugênio+Deusdará%22">de Alexandria, Francisco Eugênio Deusdará</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Rocha%2C+José+Carlos+Tatmatsu%22">Rocha, José Carlos Tatmatsu</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Parizotto%2C+Nivaldo+Antonio%22">Parizotto, Nivaldo Antonio</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Tim%2C+Carla+Roberta%22">Tim, Carla Roberta</searchLink><relatesTo>1</relatesTo><i> carlinha_tim@hotmail.com</i>
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  Data: <searchLink fieldCode="JN" term="%22Lasers+in+Medical+Science%22">Lasers in Medical Science</searchLink>. Jun2020, Vol. 35 Issue 4, p939-947. 9p.
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  Data: <searchLink fieldCode="DE" term="%22Skin+grafting%22">Skin grafting</searchLink><br /><searchLink fieldCode="DE" term="%22Animal+models+in+research%22">Animal models in research</searchLink><br /><searchLink fieldCode="DE" term="%22Histopathology%22">Histopathology</searchLink><br /><searchLink fieldCode="DE" term="%22Clinical+trials%22">Clinical trials</searchLink><br /><searchLink fieldCode="DE" term="%22Immunohistochemistry%22">Immunohistochemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Interleukins%22">Interleukins</searchLink><br /><searchLink fieldCode="DE" term="%22Collagen%22">Collagen</searchLink><br /><searchLink fieldCode="DE" term="%22Skin%22">Skin</searchLink><br /><searchLink fieldCode="DE" term="%22Cytometry%22">Cytometry</searchLink><br /><searchLink fieldCode="DE" term="%22Animal+experimentation%22">Animal experimentation</searchLink><br /><searchLink fieldCode="DE" term="%22Rats%22">Rats</searchLink><br /><searchLink fieldCode="DE" term="%22Mast+cells%22">Mast cells</searchLink><br /><searchLink fieldCode="DE" term="%22Animals%22">Animals</searchLink><br /><searchLink fieldCode="DE" term="%22Metabolism%22">Metabolism</searchLink>
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  Data: Skin graft is one of the most common techniques used in plastic surgery and repair. However, there are some complications that can lead to loss of the skin graft. Thus, several features have been studied with the aim of promoting the integration of skin grafts. Among these resources, the use of laser photobiomodulation (laser PBM) has been highlighted. The present study aimed to investigate the effects of laser PBM on the viability and integration of skin grafts in rats. Twenty male Wistar rats (± 250 g) were randomly assigned into two experimental groups with 10 animals each: control group, animals submitted to skin graft and simulation of laser PBM; laser PBM group, submitted to the skin graft and submitted to laser PBM at 660 nm, 40 mW, 60 s, 2.4 J. The animals were submitted to laser photobiomodulation immediately after the surgical procedure and each 24 h. Animal euthanasia occurred on the 7th day after surgery, 24 h after the last treatment session. The histopathological analysis revealed that the laser PBM showed better adhesion of the graft when compared to the control group. Likewise, the morphometric analysis of mast cells, blood vessels, and collagen showed a statistically significant increase in the animals irradiated with the laser PBM when compared to the control group. In addition, immunohistochemical analysis demonstrated that the laser PBM showed statistically higher immunoexpression of FGF when compared to the CG. However, IL-4 immunoexpression did not show statistical difference between the experimental groups. From the results obtained in the present study, it can be suggested that laser photobiomodulation was effective in promoting the integration and viability of total skin grafts in rats. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of Lasers in Medical Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1007/s10103-019-02909-y
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      – Code: eng
        Text: English
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        PageCount: 9
        StartPage: 939
    Subjects:
      – SubjectFull: Skin grafting
        Type: general
      – SubjectFull: Animal models in research
        Type: general
      – SubjectFull: Histopathology
        Type: general
      – SubjectFull: Clinical trials
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      – SubjectFull: Immunohistochemistry
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      – SubjectFull: Interleukins
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      – SubjectFull: Collagen
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      – SubjectFull: Skin
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      – SubjectFull: Cytometry
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      – SubjectFull: Animal experimentation
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              Text: Jun2020
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