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An integrated approach to the recovery of travertine biodegradation by combining phyto-cleaning with genomic characterization.

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Title: An integrated approach to the recovery of travertine biodegradation by combining phyto-cleaning with genomic characterization.
Authors: Genova, Chiara1,2 (AUTHOR) chiara.genova@uniroma1.it, Grottoli, Alessandro3 (AUTHOR) grottoliphd@gmail.com, Zoppis, Emma3 (AUTHOR) zoppis.1533968@studenti.uniroma1.it, Cencetti, Claudia2,4 (AUTHOR) c.cencetti@qitech.it, Matricardi, Pietro2 (AUTHOR) pietro.matricardi@uniroma1.it, Favero, Gabriele2 (AUTHOR) gabriele.favero@uniroma1.it
Source: Microchemical Journal. Jul2020, Vol. 156, pN.PAG-N.PAG. 1p.
Subjects: DNA primers, Carvacrol, Travertine, Thymol, Physiologic salines, Carbonate rocks, Essential oils, Adhesive tape
Geographic Terms: Rome
Abstract: Travertine is a sedimentary carbonate rock widely used in Roman arts and frequently subjected to biodeterioration. In this preliminary multi-analytical study, three essential oils (EOs) from T. vulgaris, O. vulgare, C. nepeta and their respective phenolic major components (thymol, carvacrol, pulegone) were selected to evaluate their biocidal potential against a multi-species biofilm grown on a travertine test wall located inside Sapienza University Campus in Rome. A preliminary characterization of the biofilm occurred through the employment of genomic methodologies. It has been confirmed the presence of microorganisms belonging to fungi, plant and bacteria kingdoms amplifying the rDNA in the characteristic regions (ITS, 18S, 16S) by performing PCR amplification with specific primers. For this purpose, two different biofilm sampling procedures, one completely non-invasive (swab in physiologic saline solution) and one micro-invasive (adhesive tape) have been employed: the adhesive tape performed better than swab in relation to DNA purity and quantity. After the biofilm characterization, the application of the phytochemicals occurred. For the realization of formulations, the EOs and their major components have been incorporated inside an innovative hydrogel matrix conceived for the cleaning of cultural heritage. The biocidal and cleaning action was evaluated with colorimetric measurements before and after the application of the products and, in the case of the surfaces treated with the essential oils, these measurements have been repeated 6 weeks after the end of the treatment. It emerged that the formulations containing the active phenolic compounds have, in most cases, a better efficacy against the multispecies biofilm. Anyway, it was verified that the biocidal action of the essential oils lasts in time, since colour variation towards the original colour of the surface have been determined 6 weeks after the end of the procedure. [ABSTRACT FROM AUTHOR]
Copyright of Microchemical Journal is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
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  Data: An integrated approach to the recovery of travertine biodegradation by combining phyto-cleaning with genomic characterization.
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  Data: <searchLink fieldCode="AR" term="%22Genova%2C+Chiara%22">Genova, Chiara</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> chiara.genova@uniroma1.it</i><br /><searchLink fieldCode="AR" term="%22Grottoli%2C+Alessandro%22">Grottoli, Alessandro</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> grottoliphd@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Zoppis%2C+Emma%22">Zoppis, Emma</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> zoppis.1533968@studenti.uniroma1.it</i><br /><searchLink fieldCode="AR" term="%22Cencetti%2C+Claudia%22">Cencetti, Claudia</searchLink><relatesTo>2,4</relatesTo> (AUTHOR)<i> c.cencetti@qitech.it</i><br /><searchLink fieldCode="AR" term="%22Matricardi%2C+Pietro%22">Matricardi, Pietro</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> pietro.matricardi@uniroma1.it</i><br /><searchLink fieldCode="AR" term="%22Favero%2C+Gabriele%22">Favero, Gabriele</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> gabriele.favero@uniroma1.it</i>
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  Data: <searchLink fieldCode="DE" term="%22DNA+primers%22">DNA primers</searchLink><br /><searchLink fieldCode="DE" term="%22Carvacrol%22">Carvacrol</searchLink><br /><searchLink fieldCode="DE" term="%22Travertine%22">Travertine</searchLink><br /><searchLink fieldCode="DE" term="%22Thymol%22">Thymol</searchLink><br /><searchLink fieldCode="DE" term="%22Physiologic+salines%22">Physiologic salines</searchLink><br /><searchLink fieldCode="DE" term="%22Carbonate+rocks%22">Carbonate rocks</searchLink><br /><searchLink fieldCode="DE" term="%22Essential+oils%22">Essential oils</searchLink><br /><searchLink fieldCode="DE" term="%22Adhesive+tape%22">Adhesive tape</searchLink>
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  Data: <searchLink fieldCode="DE" term="%22Rome%22">Rome</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Travertine is a sedimentary carbonate rock widely used in Roman arts and frequently subjected to biodeterioration. In this preliminary multi-analytical study, three essential oils (EOs) from T. vulgaris, O. vulgare, C. nepeta and their respective phenolic major components (thymol, carvacrol, pulegone) were selected to evaluate their biocidal potential against a multi-species biofilm grown on a travertine test wall located inside Sapienza University Campus in Rome. A preliminary characterization of the biofilm occurred through the employment of genomic methodologies. It has been confirmed the presence of microorganisms belonging to fungi, plant and bacteria kingdoms amplifying the rDNA in the characteristic regions (ITS, 18S, 16S) by performing PCR amplification with specific primers. For this purpose, two different biofilm sampling procedures, one completely non-invasive (swab in physiologic saline solution) and one micro-invasive (adhesive tape) have been employed: the adhesive tape performed better than swab in relation to DNA purity and quantity. After the biofilm characterization, the application of the phytochemicals occurred. For the realization of formulations, the EOs and their major components have been incorporated inside an innovative hydrogel matrix conceived for the cleaning of cultural heritage. The biocidal and cleaning action was evaluated with colorimetric measurements before and after the application of the products and, in the case of the surfaces treated with the essential oils, these measurements have been repeated 6 weeks after the end of the treatment. It emerged that the formulations containing the active phenolic compounds have, in most cases, a better efficacy against the multispecies biofilm. Anyway, it was verified that the biocidal action of the essential oils lasts in time, since colour variation towards the original colour of the surface have been determined 6 weeks after the end of the procedure. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Microchemical Journal is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.microc.2020.104918
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 1
        StartPage: N.PAG
    Subjects:
      – SubjectFull: DNA primers
        Type: general
      – SubjectFull: Carvacrol
        Type: general
      – SubjectFull: Travertine
        Type: general
      – SubjectFull: Thymol
        Type: general
      – SubjectFull: Physiologic salines
        Type: general
      – SubjectFull: Carbonate rocks
        Type: general
      – SubjectFull: Essential oils
        Type: general
      – SubjectFull: Adhesive tape
        Type: general
      – SubjectFull: Rome
        Type: general
    Titles:
      – TitleFull: An integrated approach to the recovery of travertine biodegradation by combining phyto-cleaning with genomic characterization.
        Type: main
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          Name:
            NameFull: Genova, Chiara
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            NameFull: Grottoli, Alessandro
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            NameFull: Zoppis, Emma
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            NameFull: Cencetti, Claudia
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            NameFull: Matricardi, Pietro
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            NameFull: Favero, Gabriele
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            – D: 01
              M: 07
              Text: Jul2020
              Type: published
              Y: 2020
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              Value: 156
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            – TitleFull: Microchemical Journal
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