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The impact of putative methyltransferase overexpression on the Trichoderma harzianum cellulolytic system for biomass conversion.

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Title: The impact of putative methyltransferase overexpression on the Trichoderma harzianum cellulolytic system for biomass conversion.
Authors: Delabona, Priscila da Silva1,2,3 (AUTHOR) pridelabona@yahoo.com.br, Codima, Carla Aloia1 (AUTHOR), Ramoni, Jonas1,2 (AUTHOR), Zubieta, Mariane Paludetti1 (AUTHOR), de Araújo, Bruna Martins3 (AUTHOR), Farinas, Cristiane Sanchez4 (AUTHOR), Pradella, José Geraldo da Cruz1 (AUTHOR), Seiboth, Bernhard2 (AUTHOR)
Source: Bioresource Technology. Oct2020, Vol. 313, pN.PAG-N.PAG. 1p.
Subjects: Trichoderma harzianum, Glycosidases, Multienzyme complexes, Biomass conversion, Bagasse, Cellulase, Sugar, Glucosidases
Abstract: • The role of lae1 in the cellulases production by Trichoderma harzianum. • Genes encoding enzymes involved in biomass degradation by lae1 clone overexpression. • Improvement of biomass hydrolysis using T. harzianuḿs lae1 overexpression cocktail. Trichoderma harzianum has attracting attention for its potential alternative use in biofuel production, due to a recognized competence for high diversity glycoside hydrolases (GH) enzyme complex, including higher β-glucosidases and auxiliary proteins, using low-cost carbon sources. This strain constitutively overexpressed the global regulator putative methyltransferase - LAE1, in order to improve the GHs production. The recombinant strain achieved 79-fold increase in lae1 expression and high GHs productivity. The evaluation of the LAE1 impact to induce the GHs used soluble and lignocellulose inexpensive carbon sources in a stirred-tank bioreactor. Using sugarcane bagasse with sucrose, the overexpression of lae1 resulted in significantly increment of gh61b (31x), cel7a (25x), bgl1 (20x) and xyn3 (20x) genes expression. Reducing sugar released from pretreated sugarcane bagasse, which hydrolyzed by recombinant crude enzyme cocktail, achieved 41% improvement. Therefore, lae1 overexpression effectively is a promising improving GHs target for biomass degradation by T. harzianum. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
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Abstract:• The role of lae1 in the cellulases production by Trichoderma harzianum. • Genes encoding enzymes involved in biomass degradation by lae1 clone overexpression. • Improvement of biomass hydrolysis using T. harzianuḿs lae1 overexpression cocktail. Trichoderma harzianum has attracting attention for its potential alternative use in biofuel production, due to a recognized competence for high diversity glycoside hydrolases (GH) enzyme complex, including higher β-glucosidases and auxiliary proteins, using low-cost carbon sources. This strain constitutively overexpressed the global regulator putative methyltransferase - LAE1, in order to improve the GHs production. The recombinant strain achieved 79-fold increase in lae1 expression and high GHs productivity. The evaluation of the LAE1 impact to induce the GHs used soluble and lignocellulose inexpensive carbon sources in a stirred-tank bioreactor. Using sugarcane bagasse with sucrose, the overexpression of lae1 resulted in significantly increment of gh61b (31x), cel7a (25x), bgl1 (20x) and xyn3 (20x) genes expression. Reducing sugar released from pretreated sugarcane bagasse, which hydrolyzed by recombinant crude enzyme cocktail, achieved 41% improvement. Therefore, lae1 overexpression effectively is a promising improving GHs target for biomass degradation by T. harzianum. [ABSTRACT FROM AUTHOR]
ISSN:09608524
DOI:10.1016/j.biortech.2020.123616