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The impact of putative methyltransferase overexpression on the Trichoderma harzianum cellulolytic system for biomass conversion.

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Title: The impact of putative methyltransferase overexpression on the Trichoderma harzianum cellulolytic system for biomass conversion.
Authors: Delabona, Priscila da Silva1,2,3 (AUTHOR) pridelabona@yahoo.com.br, Codima, Carla Aloia1 (AUTHOR), Ramoni, Jonas1,2 (AUTHOR), Zubieta, Mariane Paludetti1 (AUTHOR), de Araújo, Bruna Martins3 (AUTHOR), Farinas, Cristiane Sanchez4 (AUTHOR), Pradella, José Geraldo da Cruz1 (AUTHOR), Seiboth, Bernhard2 (AUTHOR)
Source: Bioresource Technology. Oct2020, Vol. 313, pN.PAG-N.PAG. 1p.
Subjects: Trichoderma harzianum, Glycosidases, Multienzyme complexes, Biomass conversion, Bagasse, Cellulase, Sugar, Glucosidases
Abstract: • The role of lae1 in the cellulases production by Trichoderma harzianum. • Genes encoding enzymes involved in biomass degradation by lae1 clone overexpression. • Improvement of biomass hydrolysis using T. harzianuḿs lae1 overexpression cocktail. Trichoderma harzianum has attracting attention for its potential alternative use in biofuel production, due to a recognized competence for high diversity glycoside hydrolases (GH) enzyme complex, including higher β-glucosidases and auxiliary proteins, using low-cost carbon sources. This strain constitutively overexpressed the global regulator putative methyltransferase - LAE1, in order to improve the GHs production. The recombinant strain achieved 79-fold increase in lae1 expression and high GHs productivity. The evaluation of the LAE1 impact to induce the GHs used soluble and lignocellulose inexpensive carbon sources in a stirred-tank bioreactor. Using sugarcane bagasse with sucrose, the overexpression of lae1 resulted in significantly increment of gh61b (31x), cel7a (25x), bgl1 (20x) and xyn3 (20x) genes expression. Reducing sugar released from pretreated sugarcane bagasse, which hydrolyzed by recombinant crude enzyme cocktail, achieved 41% improvement. Therefore, lae1 overexpression effectively is a promising improving GHs target for biomass degradation by T. harzianum. [ABSTRACT FROM AUTHOR]
Copyright of Bioresource Technology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 144624520
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
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  Label: Title
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  Data: The impact of putative methyltransferase overexpression on the Trichoderma harzianum cellulolytic system for biomass conversion.
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  Data: <searchLink fieldCode="AR" term="%22Delabona%2C+Priscila+da+Silva%22">Delabona, Priscila da Silva</searchLink><relatesTo>1,2,3</relatesTo> (AUTHOR)<i> pridelabona@yahoo.com.br</i><br /><searchLink fieldCode="AR" term="%22Codima%2C+Carla+Aloia%22">Codima, Carla Aloia</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ramoni%2C+Jonas%22">Ramoni, Jonas</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zubieta%2C+Mariane+Paludetti%22">Zubieta, Mariane Paludetti</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22de+Araújo%2C+Bruna+Martins%22">de Araújo, Bruna Martins</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Farinas%2C+Cristiane+Sanchez%22">Farinas, Cristiane Sanchez</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Pradella%2C+José+Geraldo+da+Cruz%22">Pradella, José Geraldo da Cruz</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Seiboth%2C+Bernhard%22">Seiboth, Bernhard</searchLink><relatesTo>2</relatesTo> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22Bioresource+Technology%22">Bioresource Technology</searchLink>. Oct2020, Vol. 313, pN.PAG-N.PAG. 1p.
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  Data: <searchLink fieldCode="DE" term="%22Trichoderma+harzianum%22">Trichoderma harzianum</searchLink><br /><searchLink fieldCode="DE" term="%22Glycosidases%22">Glycosidases</searchLink><br /><searchLink fieldCode="DE" term="%22Multienzyme+complexes%22">Multienzyme complexes</searchLink><br /><searchLink fieldCode="DE" term="%22Biomass+conversion%22">Biomass conversion</searchLink><br /><searchLink fieldCode="DE" term="%22Bagasse%22">Bagasse</searchLink><br /><searchLink fieldCode="DE" term="%22Cellulase%22">Cellulase</searchLink><br /><searchLink fieldCode="DE" term="%22Sugar%22">Sugar</searchLink><br /><searchLink fieldCode="DE" term="%22Glucosidases%22">Glucosidases</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: • The role of lae1 in the cellulases production by Trichoderma harzianum. • Genes encoding enzymes involved in biomass degradation by lae1 clone overexpression. • Improvement of biomass hydrolysis using T. harzianuḿs lae1 overexpression cocktail. Trichoderma harzianum has attracting attention for its potential alternative use in biofuel production, due to a recognized competence for high diversity glycoside hydrolases (GH) enzyme complex, including higher β-glucosidases and auxiliary proteins, using low-cost carbon sources. This strain constitutively overexpressed the global regulator putative methyltransferase - LAE1, in order to improve the GHs production. The recombinant strain achieved 79-fold increase in lae1 expression and high GHs productivity. The evaluation of the LAE1 impact to induce the GHs used soluble and lignocellulose inexpensive carbon sources in a stirred-tank bioreactor. Using sugarcane bagasse with sucrose, the overexpression of lae1 resulted in significantly increment of gh61b (31x), cel7a (25x), bgl1 (20x) and xyn3 (20x) genes expression. Reducing sugar released from pretreated sugarcane bagasse, which hydrolyzed by recombinant crude enzyme cocktail, achieved 41% improvement. Therefore, lae1 overexpression effectively is a promising improving GHs target for biomass degradation by T. harzianum. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Bioresource Technology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.biortech.2020.123616
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      – Code: eng
        Text: English
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      Pagination:
        PageCount: 1
        StartPage: N.PAG
    Subjects:
      – SubjectFull: Trichoderma harzianum
        Type: general
      – SubjectFull: Glycosidases
        Type: general
      – SubjectFull: Multienzyme complexes
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      – SubjectFull: Biomass conversion
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      – SubjectFull: Bagasse
        Type: general
      – SubjectFull: Cellulase
        Type: general
      – SubjectFull: Sugar
        Type: general
      – SubjectFull: Glucosidases
        Type: general
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      – TitleFull: The impact of putative methyltransferase overexpression on the Trichoderma harzianum cellulolytic system for biomass conversion.
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            NameFull: Delabona, Priscila da Silva
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            – D: 01
              M: 10
              Text: Oct2020
              Type: published
              Y: 2020
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