Temperature Dependence of High Frequency Irreversible Electroporation Evaluated in a 3D Tumor Model.
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| Title: | Temperature Dependence of High Frequency Irreversible Electroporation Evaluated in a 3D Tumor Model. |
|---|---|
| Authors: | Fesmire, Christopher C.1 (AUTHOR), Petrella, Ross A.1 (AUTHOR), Fogle, Callie A.2 (AUTHOR), Gerber, David A.3 (AUTHOR), Xing, Lei4 (AUTHOR), Sano, Michael B.1 (AUTHOR) mikesano@med.unc.edu |
| Source: | Annals of Biomedical Engineering. Aug2020, Vol. 48 Issue 8, p2233-2246. 14p. 2 Diagrams, 1 Chart, 6 Graphs. |
| Subjects: | High temperatures, Electric fields, Electroporation, Low temperatures, Cell death, Pancreatic cancer |
| Abstract: | Electroporation is a bioelectric phenomenon used to deliver target molecules into cells in vitro and irreversible electroporation (IRE) is an emerging cancer therapy used to treat inoperable tumors in situ. These phenomena are generally considered to be non-thermal in nature. In this study, a 3D tumor model was used to investigate the correlation between temperature and the effectiveness of standard clinical IRE and high frequency (H-FIRE) protocols. It was found for human glioblastoma cells that in the range of 2 to 37 °C the H-FIRE lethal electric field threshold value, which describes the minimum electric field to cause cell death, is highly dependent on temperature. Increasing the initial temperature from 2 to 37 °C resulted in a significant decrease in lethal electric field threshold from 1168 to 507 V/cm and a 139% increase in ablation size for H-FIRE burst treatments. Standard clinical protocol IRE treatments resulted in a decrease in lethal threshold from 485 to 453 V/cm and a 7% increase in ablation size over the same temperature range. Similar results were found for pancreatic cancer cells which indicate that tissue temperature may be a significant factor affecting H-FIRE ablation size and treatment planning in vivo while lower temperatures may be useful in maintaining cell viability for transfection applications. [ABSTRACT FROM AUTHOR] |
| Copyright of Annals of Biomedical Engineering is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 144921600 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Temperature Dependence of High Frequency Irreversible Electroporation Evaluated in a 3D Tumor Model. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Fesmire%2C+Christopher+C%2E%22">Fesmire, Christopher C.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Petrella%2C+Ross+A%2E%22">Petrella, Ross A.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Fogle%2C+Callie+A%2E%22">Fogle, Callie A.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Gerber%2C+David+A%2E%22">Gerber, David A.</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Xing%2C+Lei%22">Xing, Lei</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Sano%2C+Michael+B%2E%22">Sano, Michael B.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> mikesano@med.unc.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Annals+of+Biomedical+Engineering%22">Annals of Biomedical Engineering</searchLink>. Aug2020, Vol. 48 Issue 8, p2233-2246. 14p. 2 Diagrams, 1 Chart, 6 Graphs. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22High+temperatures%22">High temperatures</searchLink><br /><searchLink fieldCode="DE" term="%22Electric+fields%22">Electric fields</searchLink><br /><searchLink fieldCode="DE" term="%22Electroporation%22">Electroporation</searchLink><br /><searchLink fieldCode="DE" term="%22Low+temperatures%22">Low temperatures</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+death%22">Cell death</searchLink><br /><searchLink fieldCode="DE" term="%22Pancreatic+cancer%22">Pancreatic cancer</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Electroporation is a bioelectric phenomenon used to deliver target molecules into cells in vitro and irreversible electroporation (IRE) is an emerging cancer therapy used to treat inoperable tumors in situ. These phenomena are generally considered to be non-thermal in nature. In this study, a 3D tumor model was used to investigate the correlation between temperature and the effectiveness of standard clinical IRE and high frequency (H-FIRE) protocols. It was found for human glioblastoma cells that in the range of 2 to 37 °C the H-FIRE lethal electric field threshold value, which describes the minimum electric field to cause cell death, is highly dependent on temperature. Increasing the initial temperature from 2 to 37 °C resulted in a significant decrease in lethal electric field threshold from 1168 to 507 V/cm and a 139% increase in ablation size for H-FIRE burst treatments. Standard clinical protocol IRE treatments resulted in a decrease in lethal threshold from 485 to 453 V/cm and a 7% increase in ablation size over the same temperature range. Similar results were found for pancreatic cancer cells which indicate that tissue temperature may be a significant factor affecting H-FIRE ablation size and treatment planning in vivo while lower temperatures may be useful in maintaining cell viability for transfection applications. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Annals of Biomedical Engineering is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s10439-019-02423-w Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 14 StartPage: 2233 Subjects: – SubjectFull: High temperatures Type: general – SubjectFull: Electric fields Type: general – SubjectFull: Electroporation Type: general – SubjectFull: Low temperatures Type: general – SubjectFull: Cell death Type: general – SubjectFull: Pancreatic cancer Type: general Titles: – TitleFull: Temperature Dependence of High Frequency Irreversible Electroporation Evaluated in a 3D Tumor Model. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Fesmire, Christopher C. – PersonEntity: Name: NameFull: Petrella, Ross A. – PersonEntity: Name: NameFull: Fogle, Callie A. – PersonEntity: Name: NameFull: Gerber, David A. – PersonEntity: Name: NameFull: Xing, Lei – PersonEntity: Name: NameFull: Sano, Michael B. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 08 Text: Aug2020 Type: published Y: 2020 Identifiers: – Type: issn-print Value: 00906964 Numbering: – Type: volume Value: 48 – Type: issue Value: 8 Titles: – TitleFull: Annals of Biomedical Engineering Type: main |
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